T‐Lymphocyte Colony Growth <i>in vitro</i>: Factors Modulating Clonal Expansion

Rozenszajn, L. A.; Gol'dman, I. L.; Kalechman, Yona; Michlin, H.; Sredni, Benjamin; Zeevi, A.; Shoham, D.

doi:10.1111/j.1600-065x.1981.tb00437.x

Cited by 18 publications

(10 citation statements)

References 37 publications

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“…4A). Likewise, neither of the polyphenols tested here, alone or in combination, affected the viability of PBMC incubated for 72 h in the presence phytohemagglutinin (PHA), the classical T-lymphocyte mitogen (25), as compared to the PHA-treated control cells (Fig. 4B, upper panel).…”

Section: Resultsmentioning

confidence: 82%

Distinct Combinatorial Effects of the Plant Polyphenols Curcumin, Carnosic Acid, and Silibinin on Proliferation and Apoptosis in Acute Myeloid Leukemia Cells

Pesakhov

Khanin

Studzinski

et al. 2010

Nutrition and Cancer

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Acute myeloid leukemia (AML) is a malignancy without effective treatment for most patients.Here we demonstrate that combinations of the dietary plant polyphenols curcumin and carnosic acid, at non-cytotoxic concentrations of each agent, produced a synergistic antiproliferative effect and a massive apoptotic cell death in HL-60 and KG-1a human AML cells. In contrast, combinations of curcumin and another plant polyphenol silibinin had a predominantly additive cytostatic effect, without pronounced cytotoxicity. Neither polyphenol combination affected viability of normal human fibroblasts or proliferating and non-proliferating blood cells. Early stage of curcumin/carnosic acid-induced apoptosis was associated with cleavage (activation) of caspases-8, -9, and -3 and the proapoptotic protein Bid, but not with oxidative stress or altered levels of other Bcl-2 family proteins (Bcl-2, Bcl-xL, Mcl-1, Bax and Bak). Inhibitors of caspase-8 and -9 markedly attenuated apoptosis, indicating the involvement of both extrinsic and intrinsic apoptotic pathways. Caspase-8 inhibition abrogated Bid cleavage and strongly reduced caspase-9 activation, suggesting that the cross-talk mechanism mediated by caspase-8-dependent Bid cleavage can contribute to the activation of the intrinsic apoptotic pathway by CUR+CA. Collectively, these results suggest a mechanistic basis for the potential use of dietary plant polyphenol combinations in the treatment and prevention of AML.

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Section: Resultsmentioning

confidence: 82%

Distinct Combinatorial Effects of the Plant Polyphenols Curcumin, Carnosic Acid, and Silibinin on Proliferation and Apoptosis in Acute Myeloid Leukemia Cells

Pesakhov

Khanin

Studzinski

et al. 2010

Nutrition and Cancer

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show abstract

“…This explanation is consistent both with proposed mechanisms of stimulation of murine lymphocytes by Con A [18] and with the various experiments described here. T cell colony formation, for example, probably requires IL 2 production in mammalian systems [26], so that the reduced colony-forming capacity of LR cells is an expected consequence of their postulated inability to respond to IL2 after stimulation by Con A. With regard to IL2 production, this model for the LR defect implies that the low values of IL2 present in supernatants of Con A -stimulated LR cells are secondary to the inability of Con A to trigger proliferation of precursors of IL2-producing cells, and that the reduced response of LR cells to a given batch of supernatant is secondary to the inability of Con A to trigger generation and proliferation of IL 2-sensitive blast cells from LR small lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

Genetic control of the response of chicken T lymphocytes to concanavalin A: cellular localization of the low responder defect

Pink¹,

Vainio²

1983

Eur J Immunol

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Genetic variation in the response of chicken lymphocytes to the T cell mitogen concanavalin A (Con A) has previously been studied by assaying tritiated thymidine [( 3H]dThd) uptake of cultured cells following mitogen stimulation. Our present results show, firstly, that low [3H]dThd uptake (e.g. in cultures of Con A-stimulated cells from low responder CB, CC or G-B1-lo birds) is correlated with low proliferative activity and with reduced capacity to form Con A-dependent T cell colonies in semi-solid agar. Secondly, in cultures containing mixtures of cells from high and low responder birds, the cells from each partner respond independently to Con A (there is neither suppression of high responder, nor activation of low responder cell proliferation). Finally, Con A-stimulated cultures of high responder cells respond better to T cell growth factors, as well as producing more growth factor activity, than cultures of low responder cells. These results suggest that the basis for the low responder phenotype is an intrinsic inability of low responder T lymphocytes to respond to Con A by differentiating into growth factor-sensitive blast cells.

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“…absence of RER), we presume that they are not directly involved in the production of antibodies. Further studies are in progress, however, to determine whether they depend upon the production of growth factors from type I col onies, as suggested by Rozenszajn et al [1981], or have an independent origin.…”

Section: Discussionmentioning

confidence: 99%

“…However, a reliable method for the induction of primary antibody responses in vitro has proved very difficult to develop. PBMC can be activated by a variety of polyclonal stimulants such as phytohemagglutinin (PHA), pokeweed mitogen (PWM), concanavalin A, bacterial lipopolysaccharides and staphylococcal protein A [Bobak and Whisler, 1980;Kuritani et al, 1980;Muraguchi et al, 1980;Sredni et al, 1981], which are thought to mimic antigens [Greaves and Janossy, 1972]. These methods have yielded inter esting information, but are fraught with the problem of extrapolating the results to antigen-dependent re sponses [Collard, 1979].…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural Aspects of the Primary in vitro Antibody Response of Mononuclear Cells in Human Blood

Biasi¹,

Villa

1985

Cells Tissues Organs

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Investigations on the morphology of cells that participate in immune responses in vitro have been limited because the recovery and identification of immunocompetent cells growing dispersely in conventional liquid cultures are technically difficult and allow only the observation of individual antibody-forming cells. Here we used a system in which focal proliferation of antisheep erythrocyte antibody-secreting cells has been induced in semisolid cultures of peripheral blood mononuclear cells. By this method, intact colonies can be observed by light microscopy at the center of each hemolytic area and then processed for electron microscopy without disrupting the connections existing among the cells. Two types of colonies develop: type I colonies which grow deeply into the agar and contain cells that undergo a complete process of differentiation from blast to mature plasma cell, and type II colonies which grow more superficially and do not seem to be directly involved in antibody production.

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T‐Lymphocyte Colony Growth in vitro: Factors Modulating Clonal Expansion

Cited by 18 publications

References 37 publications

Distinct Combinatorial Effects of the Plant Polyphenols Curcumin, Carnosic Acid, and Silibinin on Proliferation and Apoptosis in Acute Myeloid Leukemia Cells

Distinct Combinatorial Effects of the Plant Polyphenols Curcumin, Carnosic Acid, and Silibinin on Proliferation and Apoptosis in Acute Myeloid Leukemia Cells

Genetic control of the response of chicken T lymphocytes to concanavalin A: cellular localization of the low responder defect

Ultrastructural Aspects of the Primary in vitro Antibody Response of Mononuclear Cells in Human Blood

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