T‐Lymphocyte Recognition of Alloantigen In Vitro

Hertel-Wulff, B; Rubin, Bent

doi:10.1111/j.1365-3083.1977.tb00414.x

Scand J Immunol

1977

DOI: 10.1111/j.1365-3083.1977.tb00414.x

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T‐Lymphocyte Recognition of Alloantigen In Vitro

B Hertel-Wulff

Bent Rubin

Abstract: Lymphocyte proliferation in vitro induced by soluble protein antigens I I. Cellular requirements*Immune guinea pig lymph node cells were fractionated on Ig anti-Ig o r HSA anti-HSA affinity columns o r on plastic surface in medium containing carbonyl iron. These techniques selectively removed B lymphocytes, K lymphocytes or adherent cells. The residual cells (Fc receptor-negative T lymphocytes) responded to soluble antigen in vitro in the same way or even better compared with nonfractionated cells. In addition… Show more

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1978

1988

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Cited by 8 publications

References 30 publications

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Expression of Fc_γ receptors on splenic T cells of mice infected with Plasmodium chabaudi adami

Langhorne¹,

Titus

1988

Eur J Immunol

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In previous studies, it was shown that mice infected with Plasmodium chabaudi adami have a deficiency in their production of IgG1 immunoglobulin, suggesting isotype-specific immunoregulation. In order to examine this phenomenon in further detail the expression of Fc gamma receptors (Fc gamma R) on T cells obtained from mice infected with P. chabaudi was studied by flow cytometry. There was an increase in the number of splenic T cells which expressed Fc gamma R during infection. At the peak of the acute stage of infection (10-15 days) up to 40% of T cells were positive for Fc gamma R expression. These Fc gamma R were present on about 40% of both Lyt-2+ and L3T4+T cells. The isotype preference of these receptors on control Thy-1+ T cells is IgG1 greater than IgG2b greater than IgG2a as determined by an inhibition assay and fluorescence-activated cell sorter (FACS) analysis. However, 2 to 3 weeks after infection this pattern was altered such that IgG2b and IgG2a represented the major isotypes binding to the Fc gamma R of the L3T4+ T cell. At this stage of infection Fc gamma R on L3T4+ cells fail to bind IgG1. In the Lyt-2% T cells IgG1 and IgG2b remained the best inhibitors. These data suggest that there may be changes in Fc gamma R expression on T cells during infection reflected particularly in a decreased ability of IgG1 to bind to the Fc gamma R of L3T4+ cells.

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Expression of Fc_γ receptors on splenic T cells of mice infected with Plasmodium chabaudi adami

Langhorne¹,

Titus

1988

Eur J Immunol

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Antigenic and genetic parameters in the stimulation and in the lytic phases of anti‐hapten + self cytotoxic T cells and their derived clones: Role of the T helper cell

Schmitt‐Verhulst

Albert

Guimezanes

et al. 1981

J. Supramol. Struct. Cell. Biochem.

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Requirements for stimulation of cytotoxic T cells (CTL) and for their lytic recognition have been compared in T cell lines repeatedly stimulated with trinitrobenzene sulfonate-treated syngeneic murine spleen cells. Differences were observed between the requirements for cells to stimulate or to be lysed by the CTL, which included: (a) the expression of major histocompatibility complex (MHC = H-2) encoded allelic products, and (b) the hapten density. Propagation of the CTL within the line required I-A intra-H-2 homology between hapten-treated stimulation cells and the line cells, whereas the lytic interaction required H-2K region homology between hapten-treated target cells and CTL. The hapten density requirement was analyzed for a responder (H-2k) and a non-responder (H-2b) strain to low hapten density modified syngeneic cells. This property was found to be a characteristic of the lytic phase rather than of the stimulation of CTL. CTL clones could be derived by growing the line cells under conditions of limiting dilution in the presence ot T cell growth factors. Such CTL clones were unable to be stimulated by their target antigens and were dependent on T cell growth factors for their propagation. These results are discussed in terms of the dependence of the development and growth of CTL on T helper cells.

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Lipoprotein changes induced by bacterial lipopolysaccharides in ‘non‐responder’ C3H/HeJ mice

1979

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T‐Lymphocyte Recognition of Alloantigen In Vitro

Cited by 8 publications

References 30 publications

Expression of Fc_γ receptors on splenic T cells of mice infected with Plasmodium chabaudi adami

Expression of Fc_γ receptors on splenic T cells of mice infected with Plasmodium chabaudi adami

Antigenic and genetic parameters in the stimulation and in the lytic phases of anti‐hapten + self cytotoxic T cells and their derived clones: Role of the T helper cell

Lipoprotein changes induced by bacterial lipopolysaccharides in ‘non‐responder’ C3H/HeJ mice

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T‐Lymphocyte Recognition of Alloantigen In Vitro

Cited by 8 publications

References 30 publications

Expression of Fcγ receptors on splenic T cells of mice infected with Plasmodium chabaudi adami

Expression of Fcγ receptors on splenic T cells of mice infected with Plasmodium chabaudi adami

Antigenic and genetic parameters in the stimulation and in the lytic phases of anti‐hapten + self cytotoxic T cells and their derived clones: Role of the T helper cell

Lipoprotein changes induced by bacterial lipopolysaccharides in ‘non‐responder’ C3H/HeJ mice

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Product

Resources

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Expression of Fc_γ receptors on splenic T cells of mice infected with Plasmodium chabaudi adami

Expression of Fc_γ receptors on splenic T cells of mice infected with Plasmodium chabaudi adami