T lymphocyte recruitment by interleukin-8 (IL-8). IL-8-induced degranulation of neutrophils releases potent chemoattractants for human T lymphocytes both in vitro and in vivo.

Abstract

IL-8 has been shown to be a human neutrophil and T cell chemoattractant in vitro. In an effort to assess the in vivo effects of IL-8 on human leukocyte migration, we examined the ability of rhIL-8 to induce human T cell infiltration using a human/mouse model in which SCID mice were administered human peripheral blood lymphocytes intraperitoneally, followed by subcutaneous injections of rhIL-8. rhIL-8 induced predominantly murine neutrophil accumulation by 4 h after administration while recombinant human macrophage inflammatory protein-1 ␤ (rhMIP-1 ␤ ) induced both murine monocytes and human T cell infiltration during the same time period as determined by immunohistology. Interestingly, 72 h after chemokine administration, a marked human T cell infiltrate was observed in the IL-8 injection site suggesting that rhIL-8 may be acting indirectly possibly through a murine neutrophil-derived T cell chemoattractant. This hypothesis was confirmed using granulocyte-depleted SCID mice. Moreover, human neutrophils stimulated in vitro with IL-8 were found to release granulederived factor(s) that induce in vitro T cell and monocyte chemotaxis and chemokinesis. This T cell and monocyte chemotactic activity was detected in extracts of both azurophilic and specific granules. Together, these results demonstrate that neutrophils store and release, upon stimulation with IL-8 or other neutrophil activators, chemoattractants that mediate T cell and monocyte accumulation at sites of inflammation. ( J. Clin Invest. 1996Invest. 97:1931Invest. -1941

The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8

“…Monocytes, the blood circulating precursors of tissue macrophages, are also recruited to infectious sites, although at a slower rate than neutrophils. They increase the initial macrophage population, with cytokines produced by neutrophils known to attract them [36,37]. It follows, therefore, that each of the two phagocyte types has the capacity to attract the other.…”

Molecular characterization and expression profiles in response to bacterial infection of Chinese soft-shelled turtle interleukin-8 (IL-8), the first reptilian chemokine gene

“…In response to both endogenous (IL-1, TNF) and exogenous (LPS) stimuli, IL-8 can be produced by many cell types (including monocyte/macrophage, neutrophil, vascular endothelial cell, epithelial cell, fibroblast, mast cell, dendritic cell, T lymphocyte, etc.) and influences a variety of leukocyte functions [7][8][9]. It mainly attracts neutrophils and induces them to release lysozomal enzymes, undergo a change in shape, trigger the respiratory burst, and increase the expression of adhesion molecules on the cell surface (reviewed in Ref.…”

“…[10]). Other properties attributed to IL-8 include the chemoattraction of T lymphocytes [8], angiogenic activity (reviewed in Ref. [11]), stimulation of histamine release from basophils (reviewed in Ref.…”

“…When screening a subtracted cDNA library from Chinese soft-shelled turtle (Trionyx sinensis) experimentally infected with Aeromonas hydrophila by suppressive subtractive hybridization (SSH), one of the most abundant genes was found to have high similarity to Chinese goose IL-8 sequence, we named it turtle IL-8 [19]. Since reptilian IL- 8 has not yet been reported, the turtle IL-8 is of particular interest for further analysis. Herein, we report the cDNA cloning, gene structure and phylogenetic analysis of soft-shelled turtle IL-8, the first reptilian chemokine, and analyze its expression profiles during bacterial infection by realtime quantitative PCR (RQ-PCR).…”

Quantum Proteolytic Activation of Chemokine CCL15 by Neutrophil Granulocytes Modulates Mononuclear Cell Adhesiveness

“…In cases of clinical cyclic or experimental neutropenia, complementary influx of mononuclear cells into inflammatory sites is significantly decreased and delayed. Replenishment of circulating neutrophils reestablishes the normal sequence of events in the development of inflammatory response (35,36). In our experimental setting, activation of neutrophils is induced on semipermeable hemofilter membranes used for hemofiltration of patients with chronic renal failure (CRF) 3 (37).…”