T Lymphocyte Subpopulations and Pokeweed Mitogen-Induced Immunoglobulin Synthesis in vitro by Mononuclear Cells from Psoriasis Patients

Abstract

T lymphocyte subpopulations and pokeweed mitogen(PWM)-induced immunoglobulin (Ig) synthesis of mononuclear cells in vitro were studied in patients with psoriasis as well as in age- and sex-matched normal blood donors. The frequences of T cells with receptors for IgG (T_g) and T cells with receptors for IgM (T_m) were examined. The IgG and IgM synthesis in mononuclear cell suspensions in the presence of different amounts of PWM was determined. The percentage of total T cells (rosetting with neuroaminidase-treated sheep red blood cells), including high and low affinity T lymphocytes showed no difference between the patient group and controls. The patients with psoriasis had a significantly higher mean proportion of T_g cells than the normal donors whereas there was no significant difference in the proportions of T_m cells between these two groups. The PWM-induced Ig synthesis in the mononuclear cell suspension seemed lower in patients with psoriasis than in controls, the difference being statistically significant when the results were expressed as ratios of Ig amounts present in the supernatants of PWM-stimulated and non-stimulated cultures (index of stimulation).

Chloroquine Stimulates the Mitogen-Driven Lymphocyte Proliferation in Patients with Psoriasis

“…These anomalies may be explained by a shift toward T suppressor cells of the T helper/T suppres sor cell ratio in the peripheral blood in psoriasis as reported 115]. There are, however, also reports suggesting a normal lymphocyte proliferation in patients with psoriasis [16][17][18]. Moreover, it has recently been found that soluble intcrlcukin-2 receptors, indicating lymphocyte activation, are elevated in psoriatic serum even after clinical remission 119-21 ].…”

Akti̇f Ve İnakti̇f Psori̇azi̇sli̇ Olgularda T Lenfosi̇t Subgruplarinin Ve Doğal Öldürücü Hücreleri̇n Değerlendi̇ri̇lmesi̇

“…Ayrıca CD4 + T hücreleri etkileyen bir baş-ka ilaç (FK 506, Takrolimus)m da etkili olması, psoriazis tedavisinde anti CD4 monoklonal antikorların kullanılması da T hücrelerinin psoriazisteki rolünü göstermektedir. Bu açıdan bakıldığında psoriazis T hücrelerinin ortaya çıkardığı anormal keratinosit proliferasyonudur (1,7,8,10,14,17).…”

“…Ancak bu bulguların deri lezyonlan düzeldiğinde normale dönmesi, saptanan bozuklukların olayın nedeni olmaktan çok hastalığın sonucu olarak ortaya çıktığını düşündürmektedir (1,2,3,5,7,8,10,12,17).…”

Distribution of circulating mononuclear cells in short-term PUVA-treated psoriatic patients and healthy subjects

“…Contradictory data have also been obtained by investigators, who used differential expression of Fe receptor binding to define T cell subpopulations. Using this method, Gu et al (18) were able to demonstrate elevated numbers of Ty cells and increased suppressor activity as defined by lowered pokeweed mitogen Ig synthesis in mononuclear cell suspension. While Acta Dem, Venereol (Stockh) 66…”