T2 Magnetic Resonance Assay-Based Direct Detection of Three Lyme Disease-Related Borrelia Species in Whole-Blood Samples

Snyder, Jessica L.; Giese, Heidi; Bandoski-Gralinski, Cheryl; Townsend, Jessica; Jacobson, Beck E.; Shivers, Robert P.; Schotthoefer, Anna M.; Fritsche, Thomas R.; Green, Clayton; Callister, Steven M.; Branda, John A.; Lowery, Thomas J.

doi:10.1128/jcm.00510-17

Cited by 11 publications

(5 citation statements)

References 31 publications

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“…Recently, T2 magnetic resonance assay was evaluated to detect Candida spp. and some bacteria in whole blood (Mylonakis et al, 2015 ; Snyder et al, 2017 ; Peker et al, 2018 ). In the present study, we evaluated the Magicplex TM Sepsis test (MP), comparing it with conventional BC.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Magicplex™ Sepsis Real-Time Test for the Rapid Diagnosis of Bloodstream Infections in Adults

Zboromyrska¹,

Cillóniz

Cobos-Trigueros

et al. 2019

Front. Cell. Infect. Microbiol.

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Sepsis is a serious health condition worldwide, affecting more than 30 million people globally each year. Blood culture (BC) is generally used to diagnose sepsis because of the low quantity of microbes occurring in the blood during such infections. However, ~50% of bloodstream infections (BSI) give negative BC, this figure being higher for sepsis, which delays the start of appropriate antimicrobial therapy. This prospective study evaluated a multiplex real-time polymerase chain reaction, the MagicplexTM Sepsis test (MP), for the detection of pathogens from whole blood, comparing it to routine BC. We analyzed 809 blood samples from 636 adult patients, with 132/809 (16.3%) of the samples positive for one or more relevant microorganism according to BC and/or MP. The sensitivity and specificity of MP were 29 and 95%, respectively, while the level of agreement between BC and MP was 87%. The rate of contaminated samples was higher for BC (10%) than MP (4.8%) (P < 0.001). Patients with only MP-positive samples were more likely to be on antimicrobial treatment (47%) than those with only BC-positive samples (18%) (P = 0.002). In summary, the MP test could be useful in some clinical setting, such as among patients on antibiotic therapy. Nevertheless, a low sensitivity demonstrated impairs its use as a part of a routine diagnostic algorithm.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Magicplex™ Sepsis Real-Time Test for the Rapid Diagnosis of Bloodstream Infections in Adults

Zboromyrska¹,

Cillóniz

Cobos-Trigueros

et al. 2019

Front. Cell. Infect. Microbiol.

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“…While standard PCR methods have limited sensitivity for LD, due to low numbers of B. burgdorferi in tissue and body fluids, various strategies can improve sensitivity without compromising specificity 32,33,37,38 . These include starting with larger specimen volumes, using target enrichment methods, and employing unconventional signal detection methods, including targeting multicopy Borrelia phage genes [39][40][41] .…”

Section: Advancements In Lyme Disease Diagnostics: Overcoming Limitat...mentioning

confidence: 99%

Overcoming the Challenges of Lyme Disease Diagnosis: The Role of Phage-based Testing

Jia,

Mijatovic,

Teulières

et al. 2023

MRAJ

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Tick-borne diseases are a growing concern worldwide, affecting both human and animal populations. Ticks are known to harbour a wide range of pathogens and are considered one of the most important vectors of diseases. Lyme disease, caused by Borrelia burgdorferi sensu lato, is the most common tick-borne disease in the US and Europe. However, accurate diagnosis of Lyme disease can be challenging due to the complex immune evasion strategies employed by Borrelia species and the limitations of existing diagnostic tests. To address this issue, researchers are exploring novel approaches, including the use of bacteriophages as diagnostic tools. Bacteriophages are highly specific and offer advantages over traditional methods for detecting bacteria, including Borrelia. In particular, the use of multicopy bacteriophages as molecular markers for Borrelia detection is a promising approach that may provide greater sensitivity than targeting single-copy bacterial genes. Nonetheless, the task of identifying trace amounts of bacteriophages in blood samples necessitates attention, and scientists are devising innovative techniques to surmount this hurdle. In summary, employing bacteriophages as a diagnostic tool for Lyme disease, by specifically targeting free circulating bacteriophages in blood samples, offers significant potential for enhancing patient outcomes and public health. However, additional rigorous scientific validation is required to definitively ascertain the efficacy and accuracy of using a phage-based methodology for detecting Borrelia in blood samples.

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“…While standard PCR methods have limited sensitivity for LD, due to low numbers of B. burgdorferi in tissue and body fluids, various strategies can improve sensitivity without compromising specificity [33,34,38,39]. These include starting with larger specimen volumes, using target enrichment methods, and employing unconventional signal detection methods, including targeting multicopy Borrelia phage genes [40][41][42]. This direct detection is particularly essential for recognizing various Borrelia strains that might not be captured through standard serology testing.…”

Section: Advancements In Lyme Disease Diagnostics: Overcoming Limitat...mentioning

confidence: 99%

Overcoming the Challenges of Lyme Disease Diagnosis: The Role of Phage-based Testing

Shan¹,

Jia²,

Mijatovic³

et al. 2023

Preprint

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Tick-borne diseases are a growing concern worldwide, affecting both human and animal populations. Ticks are known to harbor a wide range of pathogens and are considered one of the most important vectors of disease. Lyme disease, caused by Borrelia burgdorferi sensu lato, is the most common tick-borne disease in the US and Europe. However, accurate diagnosis of Lyme disease can be challenging due to the complex immune evasion strategies employed by Borrelia species and the limitations of existing diagnostic tests. To address this issue, researchers are exploring novel approaches, including the use of bacteriophages as diagnostic tools. Bacteriophages are highly specific and offer advantages over traditional methods for detecting bacteria, including Borrelia. In particular, the use of multicopy bacteriophages as molecular markers for Borrelia detection is a promising approach that may provide greater sensitivity than targeting single-copy bacterial genes. Nonetheless, the task of identifying trace amounts of bacteriophages in blood samples necessitates attention, and scientists are devising innovative techniques to surmount this hurdle. In summary, employing bacteriophages as a diagnostic tool for tick-borne diseases, such as Lyme disease, by specifically targeting free circulating bacteriophages in blood samples, offers significant potential for enhancing patient outcomes and public health.

show abstract

T2 Magnetic Resonance Assay-Based Direct Detection of Three Lyme Disease-Related Borrelia Species in Whole-Blood Samples

Cited by 11 publications

References 31 publications

Evaluation of the Magicplex™ Sepsis Real-Time Test for the Rapid Diagnosis of Bloodstream Infections in Adults

Evaluation of the Magicplex™ Sepsis Real-Time Test for the Rapid Diagnosis of Bloodstream Infections in Adults

Overcoming the Challenges of Lyme Disease Diagnosis: The Role of Phage-based Testing

Overcoming the Challenges of Lyme Disease Diagnosis: The Role of Phage-based Testing

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