T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genes

Abstract

A transforming gene isolated from T24 human bladder carcinoma cells is closely related to the BALB murine sarcoma virus (MSV) onc gene (v-bas). This transforming gene is localized to a 4.6 kilobase pair (kbp) region and is expressed as a 1.2-kbp polyadenylated transcript, which contains v-bas related sequences. Moreover, antisera known to detect the immunologically related onc gene products of BALB- and Harvey-MSVs recognized elevated levels of a related protein in T24 cells. The normal human homologue of v-bas was found to be indistinguishable from the T24 oncogene by heteroduplex and restriction enzyme analysis. These results imply that rather subtle genetic alterations have led to the activation of the normal human homologue of v-bas as a human transforming gene.

Signal transduction elements of TC21, an oncogenic member of the R-Ras subfamily of GTP-binding proteins

“…To this end, we ®rst analysed the functional consequences of TC21 expression in rodent ®broblasts and PC12, two wellknown cellular systems that respond to Ras-derived signals by undergoing morphological transformation and neuronal-like di erentiation, respectively (Bar-Sagi and Feramisco, 1985;Santos et al, 1982). The results obtained with both systems demonstrated that TC21 induces similar functional responses at the cellular level as H-Ras.…”

Myb binding sites within the N-ras promoter repress transcription

“…N-ras, Hras, and K-ras are three members of this ras family and encode similar yet non-identical proteins that have been implicated in the normal cellular processes of proliferation (Feramisco et al, 1984;Mulcahy et al, 1985) and di erentiation (Bar-Sagi and Feramisco, 1985;Noda et al, 1985;Guerrero et al, 1986), as well as the process of transformation (Der et al, 1982;Santos et al, 1982). These 21 kD proteins (p21), serve as signal transducing molecules by conformationally switching from an inactive state (GDP-bound) to an active state (GTP-bound) upon receiving a signal.…”

Transformation of mouse mammary epithelial cells with the Ha‐ras but not with the neu oncogene results in a gene dosage‐dependent increase in transforming growth factor‐α production

“…brane (ICN Biomedicals, Costa Mesa, CA) [18] and hybridized sequentially as previously described [8] to the following 32p_ labeled, nick-translated eDNA probes: a 6.6-kb BamHI fragment of a human c-Ha-ras eDNA probe [19]; a 406-bp EcoRIApaI restriction fragment derived from a human TGFa eDNA clone, pTGF-CI [20]; a 6.0-kb EcoRI/HindlII fragment of the human c-myc eDNA probe [21]; and a 770-bp human ~-actin eDNA probe (Oncor, Gaithersburg, MD).…”