T6 Is Superior to Ia (HLA-DR) As a Marker for Langerhans Cells and Indeterminate Cells in Normal Epidermis: A Monoclonal Antibody Study

Harrist, Terence J.; Muhlbauer, Jan E.; Murphy, Gëorge F.; Mihm, Martín C.; Bhan, Atul K.

doi:10.1111/1523-1747.ep12531695

Cited by 147 publications

(38 citation statements)

References 16 publications

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“…Using immunoelectron microscopy, a population of histiocytelike, OKT6-positive cells without Birbeck granules, were observed near dermal lymphatic vessels in normal human dermis and appear to be indeterminate cells (11). With immunohistochemical techniques, only a few dermal dendritic cells were observed in normal human skin (9). Our data indicates increased numbers of OKT6-positive dermal cells in the patch test specimens with the most severe inflammatory changes.…”

Section: Discussusionmentioning

confidence: 59%

“…However, we were unable to demonstrate LC in a normal skin specimen from 1 subject, even in a repeated attempt with higher concentration of the antibody; yet LC were clearly demonstrated in all other specimens from the same patient. Monoclonal T6 antibodies consistently identify more LC than similar antibodies against la-like antigens (9,10), and both methods provide better tissue morphology than ATpase histochemistry. In normal human epidermis, subsets of LC have been identified as la + T6+ and la-/T6+, suggesting that expression of la-like antigens on LC may be analogous to la inducibility on macrophages (9).…”

Section: Discussusionmentioning

confidence: 90%

“…Monoclonal T6 antibodies consistently identify more LC than similar antibodies against la-like antigens (9,10), and both methods provide better tissue morphology than ATpase histochemistry. In normal human epidermis, subsets of LC have been identified as la + T6+ and la-/T6+, suggesting that expression of la-like antigens on LC may be analogous to la inducibility on macrophages (9). Our data and one recent study (10) indicate that the expression of T6 antigen on LC is increased in allergic contact dermatitis test sites compared to adjacent normal skin.…”

Section: Discussusionmentioning

confidence: 90%

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Expression of OKT6 antigen by Langerhans cells in patch test reactions

Christensen¹,

Daniels²,

Maibach³

1986

Contact Dermatitis

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Previous studies in humans and guinea pigs indicate that Langerhans cells (LC) participate in elicitation of allergic contact dermatitis. The number and morphology of LC was determined in acute and healed allergic patch test sites, control (petrolatum) sites and normal skin in nickel-sensitive individuals using OKT6 monoclonal antibody and avidin-biotin/peroxidase labeling. Compared to normal skin, the staining intensity and number of epidermal LC was significantly increased in allergic (p < 0.02) and petrolatum control (p < 0.02) sites, and in 6-to 8-week old allergic patch test sites (p < 0.006). The in situ changes in LC induced by petrolatum alone may be a result of the occlusive patch test, or may suggest that petrolatum is not as neutral as previously believed. The nickel-induced increase in LC may indicate changes in the LC present in the epithelium at the time of testing, or migration of additional LC into the epithelium, which can then remain in situ for weeks after the antigenic challenge. The specificity of the LC increase in allergic contact dermatitis is questioned on the basis of the increase noted with petrolatum. Key words: Langerhans cells -patch test reactions.Acceptedfor publication July 15, 1985 Langerhans cells (LC) have been implicated in the induction and elicitation of allergic contact dermatitis (1). Electron microscopic studies reveal apposition of lymphocytes and LC in the epidermis and dermis of allergic but not irritant patch test reactions. Signs of LC activation and LC cell damage (appearance of glycogen granules, rarefaction of cytoplasm and dilatation of perinuclear space) occur during elicitation of contact allergic dermatitis. Utilizing the L-dopa and catecholamine method to visualize LC, Sjoborg et al. (2) showed increased numbers of LC in guinea pig and human epidermis with allergic contact dermatitis. With the same method, Uno & Hanifin (3) found a high number of LC in chronic lesions of atopic dermatitis.The monoclonal anti-T6 antibody provides * Place where study was performed.a specific (4-6) and sensitive (7) probe for LC in the skin, and the avidin-biotin/peroxidase complex (ABC) labeling method has been shown to be more sensitive than the peroxidase-antiperoxidase method in demonstrating polypeptides (8). Consequently, we applied the T6 antibody and ABC labeling to study the occurrence and appearance of LC in human patch test reactions, control patch tests without antigen, and normal skin. We also studied LC in patch test sites up to 8 weeks after antigenic challenge. Material and MethodsSubjects 7 volunteers (5 female and 2 male, 29 to 71 years old) hypersensitive to nickel (proved by earlier patch testing) enrolled in this study after giving informed consent. 4 subjects, studied for short-term changes in allergic contact test

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Section: Discussusionmentioning

confidence: 59%

Section: Discussusionmentioning

confidence: 90%

Section: Discussusionmentioning

confidence: 90%

See 1 more Smart Citation

Expression of OKT6 antigen by Langerhans cells in patch test reactions

Christensen¹,

Daniels²,

Maibach³

1986

Contact Dermatitis

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“…+ dendritic cells have been demonstrated in human [5,8,9,14], canine [10] and feline skin [10,13]. We clarified that EqT6 + dendritic cells were scattered throughout the skin lesions of "Kasen".…”

mentioning

confidence: 65%

The Kinetics of Langerhans Cells in Equine Insect Hypersensitivity "Kasen".

Kurotaki¹,

Narayama²,

Oyamada

et al. 2000

The Journal of Veterinary Medical Science

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ABSTRACT. An immunohistochemical study was carried out on the kinetics of Langerhans cells (LCs) at various pathological stages of "Kasen". Skin lesions of "Kasen" that were collected by biopsy from May to October were classified histopathologically into three stages: initial (Group I, 31 cases), developing (Group II, 50 cases) and regressing (Group III, 13 cases). LCs showed a positive reaction with antiequine thymocytes (EqT6) monoclonal antibody (MoAb) and anti-major histocompatibility complex (MHC) class II MoAb by immunohistochemical staining. The anti-EqT6 MoAb was intensely positive along the cytoplasmic process. The number of LCs per unit area increased markedly with the passage of time from the initial to the developing stage of the disease, particularly in the epidermo-dermal junction (EDJ). However, the number of LCs tended to decrease in the epidermal layer. In conclusion, the LCs moving into the epidermal layer moved into the EDJ and dermis during the time course of lesion development, and the changes occurring in LCs possibly influenced the progression of the skin lesions of "Kasen".-KEY WORDS: , , , , .

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“…Ultrathin cryosections of normal human skin were immunolabeled using either Ab1 or Ab2, and 15 nm protein A-coated gold particles as previously described (19).…”

Section: Immunoelectron Microscopymentioning

confidence: 99%

Cutting Edge: Lack of Evidence for Connexin-43 Expression in Human Epidermal Langerhans Cells

Zimmerli

Masson

Cancela

et al. 2007

The Journal of Immunology

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A provocative study has shown that viral peptides may be transferred in vitro from epithelial cells to APC through connexin-43 gap junction channels. In support of this cross-presentation pathway, the study also reported that human dendritic cells, including Langerhans cells of skin, express connexin-43. In this report we show that if this was the case, the levels of connexin-43 are below those detectable by immunofluorescence, flow cytometry, quantitative PCR of purified CD1a+ cells, and electron microscopy, raising questions about the relevance of the connexin-43-dependent mechanism for Langerhans cells of noninflamed human skin.

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T6 Is Superior to Ia (HLA-DR) As a Marker for Langerhans Cells and Indeterminate Cells in Normal Epidermis: A Monoclonal Antibody Study

Cited by 147 publications

References 16 publications

Expression of OKT6 antigen by Langerhans cells in patch test reactions

Expression of OKT6 antigen by Langerhans cells in patch test reactions

The Kinetics of Langerhans Cells in Equine Insect Hypersensitivity "Kasen".

Cutting Edge: Lack of Evidence for Connexin-43 Expression in Human Epidermal Langerhans Cells

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