T7 lytic phage-displayed peptide libraries exhibit less sequence bias than M13 filamentous phage-displayed peptide libraries

Krumpe, Lauren R H; Atkinson, Andrew; Smythers, Gary W.; Kandel, Andrea; Schumacher, Kathryn M.; McMahon, James B.; Makowski, Lee; Mori, Toshiyuki

doi:10.1002/pmic.200500606

Cited by 97 publications

(92 citation statements)

References 23 publications

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“…Another advantage is that T7 phage replicate faster than filamentous phage, which shortens experimental time. A recent study analyzed and compared the sequence diversity of the pre-made M13 phage linear 12-mer and cyclic 7-mer peptide libraries from NEB to the same type of peptide libraries constructed for display on T7 phage using the kit sold by Novagen (Krumpe et al 2006b). Through a bioinformatics-assisted computational approach, the T7 phage libraries were found to have fewer amino acid biases, increased peptide diversity, and more normal distributions of peptide net charge and hydropathy in comparison to the M13 libraries.…”

Section: Lytic Phage Displaymentioning

confidence: 99%

Phage display and molecular imaging: expanding fields of vision in living subjects

Cochran

2010

Biotechnology and Genetic Engineering Reviews

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In vivo molecular imaging enables non-invasive visualization of biological processes within living subjects, and holds great promise for diagnosis and monitoring of disease. The ability to create new agents that bind to molecular targets and deliver imaging probes to desired locations in the body is critically important to further advance this field. To address this need, phage display, an established technology for the discovery and development of novel binding agents, is increasingly becoming a key component of many molecular imaging research programs. This review discusses the expanding role played by phage display in the field of molecular imaging with a focus on in vivo applications. Furthermore, new methodological advances in phage display that can be directly applied to the discovery and development of molecular imaging agents are described. Various phage library selection strategies are summarized and compared, including selections against purified target, intact cells, and ex vivo tissue, plus in vivo homing strategies. An outline of the process for converting polypeptides obtained from phage display library selections into successful in vivo imaging agents is provided, including strategies to optimize in vivo performance. Additionally, the use To whom correspondence may be addressed (cochran1@stanford.edu) Abbreviations: scFv, single chain variable fragment; Fab, fragment antigen binding; ELISA, Enzyme-Linked Immunosorbent Assay; NEB, New England Biolabs; PET, positron emission tomography; SPECT, single photon emission computed tomography; NIR, near infrared; PEG, polyethylene glycol; SPARC, secreted protein acidic and rich in cysteine; VCAM-1, vascular cell adhesion molecule; MRI, magnetic resonance imaging; DOTA, 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid; IC 50 , half maximal inhibitory concentration; CT, computed tomography; K D , equilibrium dissociation constant; EGFR, epidermal growth factor receptor; EGFR-ECD, EGFR extracellular domain; Aβ 42 , amyloid-beta; MMP, matrix metalloprotease; uPAR, urokinase-type plasminogen activator receptor; VEGF, vascular endothelial growth factor; IL-11, Interleukin-11; IL-11αR, Interleukin-11 α-receptor. 58F.V. CoChran and J.r. CoChran of phage particles as imaging agents is also described. In the latter part of the review, a survey of phage-derived in vivo imaging agents is presented, and important recent examples are highlighted. Other imaging applications are also discussed, such as the development of peptide tags for site-specific protein labeling and the use of phage as delivery agents for reporter genes. The review concludes with a discussion of how phage display technology will continue to impact both basic science and clinical applications in the field of molecular imaging.

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Section: Lytic Phage Displaymentioning

confidence: 99%

Phage display and molecular imaging: expanding fields of vision in living subjects

Cochran

2010

Biotechnology and Genetic Engineering Reviews

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“…Moreover, Delano et al reported that the Fc-III peptide with an intramolecular disulfide bond can bind to the groove between the CH2 and CH3 domains with high affinity, potentially replacing Protein A binding (7). Krumpe et al reported a T7 phage-displayed peptide library system that showed less bias for amino acids in the displayed peptides and an increased peptide diversity compared to the M13 filamentous phage display (18). Using the T7 phage display system, Sakamoto et al identified a novel IgG-binding peptide ligand which interestingly, recognized acid conformers but not the normal conformation of human IgG (28).…”

Section: Discussionmentioning

confidence: 99%

The identification of affinity peptide ligands specific to the variable region ofhuman antibodies

et al. 2014

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Of all potential biological therapeutics, monoclonal antibody (mAb)-based therapies are becoming the dominant focus of clinical research. In particular, smaller recombinant antibody fragments such as single-chain variable fragments (scFv) have become the subject of intense focus. However, an efficient affinity ligand for antibody fragment purification has not been developed. In the present study, we designed a consensus sequence for the human antibody heavy or light chain-variable regions (Fv) based on the antibody sequences available in the ImMunoGeneTics information system (IMGT), and synthesized these consensus sequences as template Fv antibodies. We then screened peptide ligands that specifically bind to the repertoire-derived human Fv consensus antibody using a 12-mer-peptide library expressed-phage display method. Subsequently, 1 peptide for the VH template and 8 peptides for the VK template were selected as the candidate ligands after 4 rounds of panning the phage display. Using peptide-bead-based immunoprecipitation, the code-4 and code-13 peptides showed recovery rates of the VH and VK templates that were 20-30% and 40-50%, respectively. Both peptides exhibited better recovery rates for trastuzumab scFv (approximately 40%). If it were possible to identify the best combination of VH and VK-binding peptides among the ligand peptides suitable for the human mAb Fv sequence, the result could be a promising purification tool that might greatly improve the cost efficiencies of the purification process.In recent years, advances in protein research have resulted in several monoclonal antibody (mAb) therapies for cancer, infections and immunological disorders, which have increasingly become the preferred choice out of the many available biological therapeutics. In the United States which is the largest market for mAb therapeutics worldwide, more than 20 mAbs have been approved for clinical use and over 200 mAb candidates are in the clinical pipeline (21,25). Monoclonal antibody therapeutics are playing an important role in the clinical field and recombinant antibodies are predicted to account for more than 30% of all revenues in the biopharmaceutical market (1,4,22

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“…The prevalence of filamentous phage-displayed peptide library usage may be, in part, due to the initial development of the technology with filamentous bacteriophage, the commercial availability of filamentous phage-displayed peptide libraries or a combination of both or other factors. Recent research in the authors' laboratory indicated that libraries produced with lytic phage could surpass the diversity of filamentous phage-displayed peptide libraries [36]. Peptides displayed on lytic phage do not have to be compatible with host cell synthesis and secretion complexes because lytic phage assembly occurs within the Escherichia coli cytoplasm and mature phage virions are released by cell lysis.…”

Section: Phage-displayed Peptide Library Technologymentioning

confidence: 99%

“…Filamentous phage (M13, f1, and fd) has been the most common vector used in the construction of peptide phage-displayed libraries [31,33,34], although previous studies demonstrated specific and positional amino acid biases in populations of peptides randomly selected from M13 libraries because of the processes of filamentous phage morphogenesis [35,36]. Alternative phage display systems have been developed using lytic phage such as T7, T4, λ and P4 [37][38][39].…”

Section: Phage-displayed Peptide Library Technologymentioning

confidence: 99%

Potential of phage-displayed peptide library technology to identify functional targeting peptides

Krumpe

Mori²

2007

Expert Opinion on Drug Discovery

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Combinatorial peptide library technology is a valuable resource for drug discovery and development. Several peptide drugs developed through phage-displayed peptide library technology are presently in clinical trials and the authors envision that phage-displayed peptide library technology will assist in the discovery and development of many more. This review attempts to compile and summarize recent literature on targeting peptides developed through peptide library technology, with special emphasis on novel peptides with targeting capacity evaluated in vivo.

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T7 lytic phage-displayed peptide libraries exhibit less sequence bias than M13 filamentous phage-displayed peptide libraries

Cited by 97 publications

References 23 publications

Phage display and molecular imaging: expanding fields of vision in living subjects

Phage display and molecular imaging: expanding fields of vision in living subjects

The identification of affinity peptide ligands specific to the variable region ofhuman antibodies

Potential of phage-displayed peptide library technology to identify functional targeting peptides

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