T7 phage displaying latent membrane protein 1 of Epstein-Barr virus elicits humoral and cellular immune responses in rats

Gao, Jianming; Liu, Z; Huang, Ming‐Bao; Li, X; Z, Wang

doi:10.4149/av_2011_02_117

Cited by 6 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There has been increasing interest in bacteriophages as vectors for vaccine delivery [ 29 , 30 , 31 , 32 , 33 , 34 , 35 ]. They have high stability and are resistant to enzyme cleavage, high temperatures and wide pH changes.…”

Section: Discussionmentioning

confidence: 99%

Lambda phage-based vaccine induces antitumor immunity in hepatocellular carcinoma

Iwagami

Casulli

Nonomura

et al. 2017

Heliyon

View full text Add to dashboard Cite

Background and aimsHepatocellular carcinoma (HCC) is a difficult to treat tumor with a poor prognosis. Aspartate β-hydroxylase (ASPH) is a highly conserved enzyme overexpressed on the cell surface of both murine and human HCC cells.MethodsWe evaluated therapeutic effects of nanoparticle lambda (λ) phage vaccine constructs against ASPH expressing murine liver tumors. Mice were immunized before and after subcutaneous implantation of a syngeneic BNL HCC cell line. Antitumor actively was assessed by generation of antigen specific cellular immune responses and the identification of tumor infiltrating lymphocytes.ResultsProphylactic and therapeutic immunization significantly delayed HCC growth and progression. ASPH-antigen specific CD4+ and CD8+ lymphocytes were identified in the spleen of tumor bearing mice and cytotoxicity was directed against ASPH expressing BNL HCC cells. Furthermore, vaccination generated antigen specific Th1 and Th2 cytokine secretion by immune cells. There was widespread necrosis with infiltration of CD3+ and CD8+ T cells in HCC tumors of λ phage vaccinated mice compared to controls. Moreover, further confirmation of anti-tumor effects on ASPH expressing tumor cell growth were obtained in another murine syngeneic vaccine model with pulmonary metastases.ConclusionsThese observations suggest that ASPH may serve as a highly antigenic target for immunotherapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Lambda phage-based vaccine induces antitumor immunity in hepatocellular carcinoma

Iwagami

Casulli

Nonomura

et al. 2017

Heliyon

View full text Add to dashboard Cite

show abstract

“…Additionally, bacteriophages replicate rapidly and uniformly, which makes them inexpensive and sustainable for large-scale production. Studies have been carried out on the use of phages in vaccines against foot and mouth disease [210], hepatitis B [211], and Epstein-Barr virus [212], as well as for several additional infectious diseases [1]. As mentioned above, phages are capable of inducing antigen presentation, by mechanisms involving both MHC-I and MHC-II, through a process known as cross-presentation.…”

Section: Bacteriophage As Vaccinesmentioning

confidence: 99%

The Use of Bacteriophages in Biotechnology and Recent Insights into Proteomics

et al. 2022

View full text Add to dashboard Cite

Phages have certain features, such as their ability to form protein–protein interactions, that make them good candidates for use in a variety of beneficial applications, such as in human or animal health, industry, food science, food safety, and agriculture. It is essential to identify and characterize the proteins produced by particular phages in order to use these viruses in a variety of functional processes, such as bacterial detection, as vehicles for drug delivery, in vaccine development, and to combat multidrug resistant bacterial infections. Furthermore, phages can also play a major role in the design of a variety of cheap and stable sensors as well as in diagnostic assays that can either specifically identify specific compounds or detect bacteria. This article reviews recently developed phage-based techniques, such as the use of recombinant tempered phages, phage display and phage amplification-based detection. It also encompasses the application of phages as capture elements, biosensors and bioreceptors, with a special emphasis on novel bacteriophage-based mass spectrometry (MS) applications.

show abstract

“…Phages offer the advantage of rapid and uniform replication, allowing for inexpensive and sustainable production on a large scale. Phages have been researched for vaccination against foot and mouth disease, 53 hepatitis B, 54 Epstein-Barr virus, 55 and numerous other infectious diseases. Some examples are highlighted in detail below.…”

Section: Phages As a Vaccination Platform Against Infectious Diseasesmentioning

confidence: 99%

Phage display as a tool for vaccine and immunotherapy development

Hess

Jewell

2019

Bioengineering & Transla Med

View full text Add to dashboard Cite

Bacteriophages, or phages, are viruses that specifically infect bacteria and coopt the cellular machinery to create more phage proteins, eventually resulting in the release of new phage particles. Phages are heavily utilized in bioengineering for applications ranging from tissue engineering scaffolds to immune signal delivery. Of specific interest to vaccines and immunotherapies, phages have demonstrated an ability to activate both the innate and adaptive immune systems. The genome of these viral particles can be harnessed for DNA vaccination, or the surface proteins can be exploited for antigen display. More specifically, genes that encode an antigen of interest can be spliced into the phage genome, allowing antigenic proteins or peptides to be displayed by fusion to phage capsid proteins. Phages therefore present antigens to immune cells in a highly ordered and repetitive manner. This review discusses the use of phage with adjuvanting activity as antigen delivery vehicles for vaccination against infectious disease and cancer. K E Y W O R D S bacteriophage, biomaterials, drug delivery, immunology, nanotechnology, phage display, vaccine 1 | INTRODUCTION Bacteriophages, or phages, are prokaryotic viruses that specifically infect bacteria, and are the most abundant life form on earth. 1 Phages were first discovered in the early 20th century and noted for their antibacterial activity. The practice of administering phages (i.e., phage therapy) to patients suffering from bacterial infections began shortly after their discovery, but was controversial largely due to lack of mechanistic knowledge and variable success rates. In fact, the treatment was largely abandoned upon the discovery of antibiotics. 2 Research in this field was reenergized, however, following the development of phage display systems in 1985. 3 George Smith, a chemist at the University of Missouri, demonstrated fusion of peptides to the outer (i.e., capsid) proteins of phages enabling surface display. This work, for which Smith shared a Nobel Prize in 2018, laid the ground work for affinity selection, epitope mapping, and antibody discovery. Since this time, phages have been an important tool for the bioengineering field, exploited for a range of applications including theranostics, 4 batteries, 5,6 drug delivery, 7 and vaccine development. 1Phages are one of many nanotechnologies being investigated for these applications. This review will focus on the advantages that phages provide to the nanomedicine field, specifically vaccination and immunotherapy. Nanomedicine ranges from diagnostics to treatments and relies on the fields of biomedical and chemical engineering,

show abstract

T7 phage displaying latent membrane protein 1 of Epstein-Barr virus elicits humoral and cellular immune responses in rats

Cited by 6 publications

References 22 publications

Lambda phage-based vaccine induces antitumor immunity in hepatocellular carcinoma

Lambda phage-based vaccine induces antitumor immunity in hepatocellular carcinoma

The Use of Bacteriophages in Biotechnology and Recent Insights into Proteomics

Phage display as a tool for vaccine and immunotherapy development

Contact Info

Product

Resources

About