T929I and K1774N mutation pair and M918L single mutation identified in the voltage-gated sodium channel gene of pyrethroid-resistant Thrips tabaci (Thysanoptera: Thripidae) in Japan

Jouraku, Akiya; Kuwazaki, Seigo; Iida, Hiroyuki; Ohta, I.; Kusano, Hisao; Takagi, Motonori; Yokoyama, Tomoya; Kubota, Naoya; Shibao, Manabu; Shirotsuka, Kanako; Iwasaki, Arihiro; Takezawa, Yuji; Takeda, Mitsuyoshi

doi:10.1016/j.pestbp.2019.04.012

Cited by 12 publications

(11 citation statements)

References 44 publications

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“…K1774N was first identified in T. tabaci, paired with T929I. 18,45 We did not find the mutations (1014 and 918) in M. usitatus, which exist in many insect resistance populations. Leucine was be replaced by different amino acids (phenylalanine, cysteine, histidine, serine, and tryptophan) at position 1014, and methionine was replaced by different amino acids (threonine, isoleucine, leucine, and valine) at position 918.…”

Section: Discussioncontrasting

confidence: 60%

“…The pyrethroid-resistant strains of T. tabaci had the classic mutation T929I and a new mutation, K1774N, which are located in the domain IIS5 transmembrane region and IVS5−IVS6 linker region. 18 T929I related to resistance of pyrethroids has been verified in the sodium channel of diamondback moth, Plutella xylostella (L.), by a two-electrode voltage clamp. 19 A pyrethroidsusceptible sodium channel variant from HK-S, MuNa v 1-1, was modified to two single mutations (T929I and K1774N) and one double mutation (T929I/K1774N).…”

Section: Introductionmentioning

confidence: 95%

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Functional Characterization of Double Mutations T929I/K1774N in the Voltage-Gated Sodium Channel of Megalurothrips usitatus (Bagnall) Related to Pyrethroid Resistance

Gao,

Ma,

Geng

et al. 2024

J. Agric. Food Chem.

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Megalurothrips usitatus (Bagnall), the main pest on legume vegetables, is controlled by pyrethroids in the field. Field strains of M. usitatus resistant to pyrethroids were collected from three areas in Hainan Province (Haikou, Ledong, and Sanya City), and two mutations, T929I and K1774N, were detected in the voltage-gated sodium channel. In this study, the sodium channel in M. usitatus was first subcloned and successfully expressed in Xenopus oocytes. The single mutation (T929I or K1774N) and double mutation (T929I/K1774N) shifted the voltage dependence of activation in the hyperpolarization direction. The three mutants all reduced the amplitude of tail currents induced by type I (permethrin and bifenthrin) and type II (deltamethrin and λ-cyhalothrin) pyrethroids. Homology modeling analysis of these two mutations shows that they may change the local hydrophobicity and positive charge of the sodium channel. Our data can be used to reveal the causes of the resistance of M. usitatus to pyrethroids and provide guidance for the comprehensive control of M. usitatus in the future.

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Section: Discussioncontrasting

confidence: 60%

Section: Introductionmentioning

confidence: 95%

Functional Characterization of Double Mutations T929I/K1774N in the Voltage-Gated Sodium Channel of Megalurothrips usitatus (Bagnall) Related to Pyrethroid Resistance

Gao,

Ma,

Geng

et al. 2024

J. Agric. Food Chem.

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“…Pyrethroid resistance has been reported in thrips, such as T. tabaci, F. occidentalis, and T. palmi [20,35,42]. The M918T, L1014F, and T929I had been reported in several pyrethroidresistant T. tabaci strains in Japan [21].…”

Section: Discussionmentioning

confidence: 99%

“…The qualified DNA-Seq reads of the 30 biological replicates of each M. usitatus population were mapped to the cloned partial Nav channel gene sequence of the Haikou, Ledong, and Sanya field population using Bowtie2.2.2.6. Single nucleotide polymorphism/insertion-deletion (SNP/INDEL) calling was performed with SAM tools 0.1.19 using the mapping results and then mutations and their genotype were identified in each replicate by using in-house Perl scripts [35].…”

Section: Dna Extraction and Sodium Channel Cloning Of M Usitatusmentioning

confidence: 99%

Detection of Putative Mutation I873S in the Sodium Channel of Megalurothrips usitatus (Bagnall) Which May Be Associated with Pyrethroid Resistance

Gao

Qiu

et al. 2023

Insects

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Pyrethroid resistance of thrips has been reported in many countries, and knockdown resistance (kdr) has been identified as a main mechanism against pyrethroids in many insects. To characterize pyrethroid resistance in Megalurothrips usitatus from the Hainan Province of China, we conducted a biological assay and sequenced the voltage-gated sodium channel gene domain II from M. usitatus field populations. It showed high resistance to the pyrethroids for 2019 and 2020, in which LC50 to lambda-cyhalothrin of M. usitatus was 1683.521 mg/L from Sanya in 2020. The LC50 value of deltamethrin was lower in Haikou than in other locations, which mean the south of Hainan has higher resistance than the north of Hainan. Two mutations of I873S and V1015M were detected in the domain II region of the sodium channel in M. usitatus; however, the mutation frequency of V1015M was only 3.33% and that of I873S was 100%. One is homozygous and the other is a heterozygous mutant type. The three thrips-sensitive strains of sodium channel 873 are highly conserved in amino acids (isoleucine), while the M. usitatus pyrethroid-resistant strains are all serine, so I873S may be related to the resistance of M. usitatus to pyrethroids. The present study will contribute to the understanding of the evolution of pyrethroids resistance and contribute to the development of resistance management of M. usitatus in Hainan.

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“…Two known pyrethroid‐resistance‐related double mutations M918T/L1014F and M918L/V1010A have been identified in T. tabaci (Toda and Morishita 2009). Double mutation T929I/K1774N was found in T. tabaci , which was closely associated with pyrethroid resistance in Japan, and two single amino acid mutations T929I and M918L were identified for the first time (Jouraku et al, 2019; Misato et al, 2016; Wu et al, 2014). F. occidentalis acquire resistance due to L1014F and T929C mutations in the VGSC (Forcioli et al, 2002), and T. palmi developed resistance to pyrethroids because of mutations in M843I, A1231D, and T945I (Bao & Sonoda, 2012).…”

Section: Discussionmentioning

confidence: 99%

Double sodium channel mutation, I265T/L1014F, is possibly related to pyrethroid‐resistant in Thrips palmi

Yuan

Jin

et al. 2023

Arch Insect Biochem Physiol

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Thrips palmi Karny (Thysanoptera: Thripidae) can harm a variety of agricultural crops and transmit plant viruses, causing heavy economic losses. In the Hainan province of China, pyrethroids were sprayed widely to control T. palmi, which leaded to resistance to pyrethroids in T. palmi. The bioassay has shown that the resistance ratio of T. palmi to pyrethroids increases annually. Resistance ratio to λ‐cyhalothrin has increased from 10.711 to 23.321 and to cypermethrin has increased from 5.507 to 23.051 for 3 years, 2020−2022. The double mutation (I265T/L1014F) was identified from the field strain for the first time, which were located in the domains I and II of the voltage‐gated sodium channel of T. palmi, respectively. The double mutation is probably the reason for the higher resistance of T. palmi in Hainan. The frequencies of the double mutation were 53.33% in HN2020, 70.00% in HN2021, and 96.67% in HN2022. Results indicated that T. palmi had developed different degrees of resistance to pyrethroids in Hainan. This study provides theoretical guidance for the use of insecticides in the field control of thrips.

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T929I and K1774N mutation pair and M918L single mutation identified in the voltage-gated sodium channel gene of pyrethroid-resistant Thrips tabaci (Thysanoptera: Thripidae) in Japan

Cited by 12 publications

References 44 publications

Functional Characterization of Double Mutations T929I/K1774N in the Voltage-Gated Sodium Channel of Megalurothrips usitatus (Bagnall) Related to Pyrethroid Resistance

Functional Characterization of Double Mutations T929I/K1774N in the Voltage-Gated Sodium Channel of Megalurothrips usitatus (Bagnall) Related to Pyrethroid Resistance

Detection of Putative Mutation I873S in the Sodium Channel of Megalurothrips usitatus (Bagnall) Which May Be Associated with Pyrethroid Resistance

Double sodium channel mutation, I265T/L1014F, is possibly related to pyrethroid‐resistant in Thrips palmi

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