Table of the Integrals - C(h,O), S(h,O), E(h,O), C(h, h), S(h,h), and E(h,h) for Complex Arguments

Abstract

A purification method for a-mannosidase from the seeds of Phaseolus vulgaris is described. The main steps are ammonium sulfate precipitation, DEAE-Sephadex ion-exchange chromatography , G-200 gel filtration, and isoelectric focusing. Two enzymes, a-mannosidase I and 11, with isoleectric points of 5.1 and 6.1 respectively, were found. Most of the a-mannosidase activity was present as a-mannosidase I. This enzyme has been extensively purified. According to the criteria of disc electrophoresis and sedimentation equilibrium , the final enzyme preparation was pure. a-Mannosidase I has a sedimentation coefficient of 11.2 S, and molecular weight of 194000. The enzyme activity was shown to depend on zinc. The pH optimum and Km value have been determined with p-nitrophenyl-aD -mannoside as substrate. a-Mannosidase I1 was only partially purified and some of its properties were studied in order to compare the two enzymes. a-Mannosidase, widely distributed in animal tissue , microorganisms , and plants, is abundantly present in the seeds of Phaseolus vulgaris [ l ] and Jack beans [2]. The enzyme has been partially purified from Phaseolus vulgaris [l] and more extensively from Jack bean meal [3]. The effect of the enzyme on mannoside bonds has been studied with different oligosaccharides, polysaccharides, and glyco-proteins [3]. So far, there have been no reports on the molecular characteristics. The present paper describes the extensive purification of the enzyme from Phaseolus vulgaris, and the determination of some of its physical properties.

Vitamin D-dependency Rickets in Institutionalized, Mentally Retarded Children on Long Term Anticonvulsant Therapy. II. The Response to 25-Hydroxycholecalciferol and to Vitamin D2

“…Harrison and Harrison [18] found similar results with other anticonvulsants. It would seem from the above that there may be other contributing factors in institutionalized patients who develop vitamin D-dependency rickets while taking long term anticonvulsant drugs.…”

Theoretical Studies of an Atmosphere Around Saturn's Rings

“…As pointed out by Harrison and Schoen (1967), the bombardment by the solar wind can be an important source of mass loss for the rings. To evaluate the ejection rate more precisely, we extrapolate the results used for metals by, e.g., Dienes and Vineyard (1957) or Pease (1960) to the water ice case.…”

“…In this case, the flux to be used is that below 1450 A. The input at 9.54 AU is then 5.5 x 10 9 photons cm -2 s _1 (Hinteregger, 1970). Assuming that once a photon of A < 1450 A penetrates below a radial distance of, say, 10000 km, it produces a H atom, the total number of H atoms produced is then 1.5 x 10 28 s" 1 .…”

The Effect of Prednisolone® upon the Metabolism and Action of 25-Hydroxy and 1,25-Dihydroxyvitamin D 3

“…Adrenal glucocorticoids inhibit the intestinal absorption of calcium (1)(2)(3)(4)(5)(6)(7)(8)(9)(10). They may act by antagonizing the action of vitamin D (5,10).…”

“…They may act by antagonizing the action of vitamin D (5,10). However, administration of either vitamin Da or 25-hydroxyvitamin D8 (25-OH D3) does not reverse the inhibitory effect of the corticoids (5-7).…”