TACC3 is a microtubule plus end–tracking protein that promotes axon elongation and also regulates microtubule plus end dynamics in multiple embryonic cell types

Nwagbara, Belinda U.; Faris, Anna; Bearce, Elizabeth; Erdoğan, Burcu; Ebbert, Patrick T.; Evans, Matthew F.; Rutherford, Erin L.; Enzenbacher, Tiffany; Lowery, Laura Anne

doi:10.1091/mbc.e14-06-1121

Cited by 57 publications

(114 citation statements)

References 49 publications

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“…3C). These results were similar to previous MT dynamics effects by TACC3 OE [Nwagbara et al, 2014] (and also shown in Figs. 3A–3C), suggesting that TACC1 may modulate MT plus‐end dynamics through a mechanism related to that of TACC3.…”

Section: Resultssupporting

confidence: 93%

“…1O). While these are imprecise measurements based on experimental limitations, it is notable that they are consistent with the previous measurement of 0.5 μm between mKate2‐EB1 and GFP‐TACC3 [Nwagbara et al, 2014]. Additionally, we observed that the GFP‐TACC1 plus‐end accumulations were considerably lengthened upon overexpression of mKate2‐TACC3 (Fig.…”

Section: Resultssupporting

confidence: 91%

“…We previously demonstrated that the C‐terminal domain of TACC3 is required for MT plus‐end tracking in Xenopus [Nwagbara et al, 2014]. Since this domain is well‐conserved between TACC1 and TACC3 (Fig.…”

Section: Resultsmentioning

confidence: 95%

“…We then quantified parameters of MT polymerization dynamics using the Matlab‐based open source software, plusTipTracker [Applegate et al, 2011]. This software program has been validated for accurate tracking of fluorescently tagged EB1 comets in Xenopus laevis embryonic fibroblasts and other cell types, using similar imaging conditions to those used in the present study [Nwagbara et al, 2014; Stout et al, 2014]. mKate2‐EB1 comet velocity was on average 24% faster upon TACC1 overexpression (OE) (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Previously, we reported that the centrosome‐associated protein TACC3, a member of the transforming acidic coiled‐coil (TACC) domain family, can act as a +TIP in multiple Xenopus embryonic cell types [Nwagbara et al, 2014]. This +TIP function of TACC3 was recently confirmed to be conserved in human cells as well [Gutierrez‐Caballero et al, 2015].…”

Section: Introductionmentioning

confidence: 89%

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Xenopus TACC1 is a microtubule plus‐end tracking protein that can regulate microtubule dynamics during embryonic development

et al. 2015

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Microtubule plus‐end dynamics are regulated by a family of proteins called plus‐end tracking proteins (+TIPs). We recently demonstrated that the transforming acidic coiled‐coil (TACC) domain family member, TACC3, can function as a +TIP to regulate microtubule dynamics in Xenopus laevis embryonic cells. Although it has been previously reported that TACC3 is the only TACC family member that exists in Xenopus, our examination of its genome determined that Xenopus, like all other vertebrates, contains three TACC family members. Here, we investigate the localization and function of Xenopus TACC1, the founding member of the TACC family. We demonstrate that it can act as a +TIP to regulate microtubule dynamics, and that the conserved C‐terminal TACC domain is required for its localization to plus‐ends. We also show that, in Xenopus embryonic mesenchymal cells, TACC1 and TACC3 are each required for maintaining normal microtubule growth speed but exhibit some functional redundancy in the regulation of microtubule growth lifetime. Given the conservation of TACC1 in Xenopus and other vertebrates, we propose that Xenopus laevis is a useful system to investigate unexplored cell biological functions of TACC1 and other TACC family members in the regulation of microtubule dynamics. © 2015 The Authors. Cytoskeleton, Published by Wiley Periodicals, Inc.

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 89%

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Xenopus TACC1 is a microtubule plus‐end tracking protein that can regulate microtubule dynamics during embryonic development

et al. 2015

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Cytoskeletal social networking in the growth cone: How +TIPs mediate microtubule‐actin cross‐linking to drive axon outgrowth and guidance

2016

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The growth cone is a unique structure capable of guiding axons to their proper destinations. Within the growth cone, extracellular guidance cues are interpreted and then transduced into physical changes in the actin filament (F-actin) and microtubule cytoskeletons, providing direction and movement. While both cytoskeletal networks individually possess important growth cone-specific functions, recent data over the past several years point towards a more cooperative role between the two systems. Facilitating this interaction between F-actin and microtubules, microtubule plus-end tracking proteins (+TIPs) have been shown to link the two cytoskeletons together. Evidence suggests that many +TIPs can couple microtubules to F-actin dynamics, supporting both microtubule advance and retraction in the growth cone periphery. In addition, growing in vitro and in vivo data support a secondary role for +TIPs in which they may participate as F-actin nucleators, thus directly influencing F-actin dynamics and organization. This review focuses on how +TIPs may link F-actin and microtubules together in the growth cone, and how these interactions may influence axon guidance.

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The role of TACC3 in mitotic spindle organization

et al. 2017

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TACC3 regulates spindle organization during mitosis and also regulates centrosome-mediated microtubule nucleation by affecting g-Tubulin ring complexes. In addition, it interacts with different proteins (such as ch-TOG, clathrin and Aurora-A) to function in mitotic spindle assembly and stability. By forming the TACC3/ch-TOG complex, TACC3 acts as a plus end-tracking protein to promote microtubule elongation. The TACC3/ch-TOG/clathrin complex is formed to stabilize kinetochore fibers by crosslinking adjacent microtubules. Furthermore, the phosphorylation of TACC3 by Aurora-A is important for the formation of TACC3/ch-TOG/clathrin and its recruitment to kinetochore fibers. Recently, the aberrant expression of TACC3 in a variety of human cancers has been linked with mitotic defects. Thus, in this review, we will discuss our current understanding of the biological roles of TACC3 in mitotic spindle organization. K E Y W O R D Scancer, centrosome, microtubule, spindle, TACC3

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TACC3 is a microtubule plus end–tracking protein that promotes axon elongation and also regulates microtubule plus end dynamics in multiple embryonic cell types

Cited by 57 publications

References 49 publications

Xenopus TACC1 is a microtubule plus‐end tracking protein that can regulate microtubule dynamics during embryonic development

Xenopus TACC1 is a microtubule plus‐end tracking protein that can regulate microtubule dynamics during embryonic development

Cytoskeletal social networking in the growth cone: How +TIPs mediate microtubule‐actin cross‐linking to drive axon outgrowth and guidance

The role of TACC3 in mitotic spindle organization

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