Tachyphylaxis to inhaled isoproterenol and the effect of methylprednisolone in dogs

Stephan, William; Chick, Thomas W.; Avner, Barry P.; Jenne, John W.

doi:10.1016/0091-6749(80)90193-1

Cited by 38 publications

(5 citation statements)

References 19 publications

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“…controversial with respect to our understanding of its mechanisms. Beta-receptor desensitization has been demonstrated as a result of in vivo and in vitro treatments in rat, guinea pig, dog, and human airway smooth muscle preparations (Lin et al, 1977;Avner et al, 1977;Avner and Noland, 1978;Avner and Jenne, 1977;Jenne et al, 1977;Nelson et al, 1977;Stephen et al, 1980;Avner et al, 1980.) Desensitization of airway smooth muscle is associated with a decrease of beta-receptor affinity (i.e., the strength of binding of a drug to a membrane site) without an apparent decrease in beta-receptor number (the quantity of sites represented on the surface membrane being investigated which, when activated, produces maximum relaxation) (Lin et al, 1977;Avner and Noland, 1978).…”

Section: Discussionmentioning

confidence: 99%

A method for culturing canine tracheal smooth muscle cells in vitro: Morphologic and pharmacologic observations

et al. 1981

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A method of culturing canine tracheal smooth muscle cells in vitro is described. The morphology of these cells is monitored up to 60 days in culture and selected stages are illustrated. The characteristics of these cells are numerous mechanical attachments, the presence of thick filaments in suitably processed cells, and their contractile response to in vitro administration of carbachol, a cholinomimetic drug. They also possess nexus formations and both thin (actin) filaments and 10-nm filaments. Mitosis is found in the nonconfluent preparations up to 16 days after culturing. Cultures of 2 to 8 days appear to be most useful as pharmacological test vehicles. This system will be used to explore the phenomenon of adrenergic beta-2 receptor desensitization in airway smooth muscle, to attempt to localize these receptor sites and to determine how receptor affinity and/or number may be regulating cell response to pharmacologic agents.

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Section: Discussionmentioning

confidence: 99%

A method for culturing canine tracheal smooth muscle cells in vitro: Morphologic and pharmacologic observations

et al. 1981

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“…In intact, tachyphylactic hu man lung, as well as in isolated tachyphylactic human bronchial muscle, hydrocortisone restores responsiveness to subsequent catecholamine challenge (9,23). Similarly in dogs, methylprednisolone reverses the state of bronchial tachyphylaxis (48).…”

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confidence: 99%

Regulation of Beta-Adrenergic Receptors by Steroid Hormones

Davies¹

1984

Annual Review of Physiology

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Adrenal steroid hormones and catecholamines play central roles both in main taining survival in times of stress and in regulating normal physiologic respon siveness. The interplay between these two hormonal systems is of great physiological importance. Whereas catecholamines exert highly specific effects on specific tissues, steroid hormones have rather broad effects upon many physiologic functions in many tissues. The general pattern observed is one of steroid hormone-induced refinement or regulation of catecholamine mediated processes. In this chapter we review what is known about the mechanisms by which adrenal steroid hormones regulate the action of the j3-adrenergic receptor-adenylate cyclase system. Effects on the a-adrenergic receptors and the effects of gonadal steroids are not addressed (13,29). PHYSIOLOGIC MANIFESTATIONS OF STEROID-INDUCED I3-ADRENERGIC RECEPTOR REGULATIONSteroid hormones enhance several j3-adrenergic actions at the tissue level. Myocardialj3l-adrenergic responses such as positive inotropism (3,25), posi-119

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“…Hydrocortisone administration has been shown to return desensitized human bronchial tissue to a normal degree of relaxation (38). A similar effect of methylprednisolone exists in dogs (7). Thus, our observations provide an insight into the physiologically observable attenuating effects of steroids upon catecholamine-induced desensitization.…”

Section: Resultsmentioning

confidence: 66%

“…Dog lungs undergo desensitization of the bronchodilatory response to isoproterenol upon prolonged isoproterenol exposure. Methylprednisolone administration restores isoproterenol responsiveness in the dog lung (7). The relationship between these opposing regulatory influences is of interest, since both catecholamines and steroid hormones are secreted in acute and chronic states of stress.…”

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confidence: 99%

In Vitro Desensitization of Beta Adrenergic Receptors in Human Neutrophils. ATTENUATION BY CORTICOSTEROIDS

Davies¹,

Lefkowitz²

1983

J. Clin. Invest.

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Tachyphylaxis to inhaled isoproterenol and the effect of methylprednisolone in dogs

Cited by 38 publications

References 19 publications

A method for culturing canine tracheal smooth muscle cells in vitro: Morphologic and pharmacologic observations

A method for culturing canine tracheal smooth muscle cells in vitro: Morphologic and pharmacologic observations

Regulation of Beta-Adrenergic Receptors by Steroid Hormones

In Vitro Desensitization of Beta Adrenergic Receptors in Human Neutrophils. ATTENUATION BY CORTICOSTEROIDS

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