Tackling the challenges facing the clinical applications of pure PEO hydroxyapatite layers: Co-deposition of YSZ nanoparticles

Hosseini, Milad; Khalil‐Allafi, Jafar; Etminanfar, Mohamadreza; Safavi, Mir Saman; Bloise, Nora; Ghalandarzadeh, Arash

doi:10.1016/j.matchemphys.2022.126899

Cited by 20 publications

(3 citation statements)

References 75 publications

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“…In order to assess cell viability, cell mitochondrial activity was evaluated after 24 h and 4 days of culture with a 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide assay (MTT; Sigma-Aldrich, St. Louis, MO, USA), as previously described [32]. Absorbance was measured at 570 nm using 100 µL samples and a CLARIOstar ® Plus Multi-mode Microplate Reader (BMG Labtech, Ortenberg, Germany).…”

Section: Cell Viabilitymentioning

confidence: 99%

Combined Effects of HA Concentration and Unit Cell Geometry on the Biomechanical Behavior of PCL/HA Scaffold for Tissue Engineering Applications Produced by LPBF

Gatto,

Furlani,

Giuliani

et al. 2023

Materials

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This experimental study aims at filling the gap in the literature concerning the combined effects of hydroxyapatite (HA) concentration and elementary unit cell geometry on the biomechanical performances of additively manufactured polycaprolactone/hydroxyapatite (PCL/HA) scaffolds for tissue engineering applications. Scaffolds produced by laser powder bed fusion (LPBF) with diamond (DO) and rhombic dodecahedron (RD) elementary unit cells and HA concentrations of 5, 30 and 50 wt.% were subjected to structural, mechanical and biological characterization to investigate the biomechanical and degradative behavior from the perspective of bone tissue regeneration. Haralick’s features describing surface pattern, correlation between micro- and macro-structural properties and human mesenchymal stem cell (hMSC) viability and proliferation have been considered. Experimental results showed that HA has negative influence on scaffold compaction under compression, while on the contrary it has a positive effect on hMSC adhesion. The unit cell geometry influences the mechanical response in the plastic regime and also has an effect on the cell proliferation. Finally, both HA concentration and elementary unit cell geometry affect the scaffold elastic deformation behavior as well as the amount of micro-porosity which, in turn, influences the scaffold degradation rate.

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Section: Cell Viabilitymentioning

confidence: 99%

Combined Effects of HA Concentration and Unit Cell Geometry on the Biomechanical Behavior of PCL/HA Scaffold for Tissue Engineering Applications Produced by LPBF

Gatto,

Furlani,

Giuliani

et al. 2023

Materials

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“…The key to implant stability is osseointegration, the direct bonding of the implant material with surrounding bone [3]. Titanium is the current standard for dental implants due to its biocompatibility, strength, and corrosion resistance [4]. However, concerns exist regarding potential allergic reactions and tissue penetration with titanium implants [5].…”

Section: Introductionmentioning

confidence: 99%

Tailoring zirconia surface topography via femtosecond laser-induced nanoscale features: effects on osteoblast cells and antibacterial properties

Ghalandarzadeh,

Ganjali,

Hosseini

2024

Biomed. Mater.

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The performance and long-term durability of dental implants hinge on the quality of bone integration and their resistance to bacteria. This research aims to introduce a surface modification strategy for zirconia implants utilizing femtosecond laser ablation techniques, exploring their impact on osteoblast cell behavior and bacterial performance, as well as the integral factors influencing the soft tissue quality surrounding dental implants. Ultrafast lasers were employed to craft nanoscale groove geometries on zirconia surfaces, with thorough analyses conducted using x-ray diffraction, scanning electron microscopy, atomic force microscopy, and water contact angle measurements. The study evaluated the response of human fetal osteoblastic cell lines to textured zirconia ceramics by assessing alkaline phosphatase activity, collagen I, and interleukin 1β secretion over a 7 day period. Additionally, the antibacterial behavior of the textured surfaces was investigated using Fusobacterium nucleatum, a common culprit in infections associated with dental implants. Ciprofloxacin (CIP), a widely used antibacterial antibiotic, was loaded onto zirconia ceramic surfaces. The results of this study unveiled a substantial reduction in bacterial adhesion on textured zirconia surfaces. The fine biocompatibility of these surfaces was confirmed through the MTT assay and observations of cell morphology. Moreover, the human fetal osteoblastic cell line exhibited extensive spreading and secreted elevated levels of collagen I and interleukin 1β in the modified samples. Drug release evaluations demonstrated sustained CIP release through a diffusion mechanism, showcasing excellent antibacterial activity against pathogenic bacteria, including Streptococcus mutans, Pseudomonas aeruginosa, and Escherichia coli.

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“…With the rapid development of medical materials, bioceramic materials specifically zirconia due to their unique properties, including favorable mechanical properties, excellent chemical inertness, exceptional abrasion resistance and biocompatible properties are gradually replacing conventional polymers and metals in the manufacture of dental implants [1][2][3][4][5][6]. Numerous studies have demonstrated that zirconia ceramics are ideal for dental implants due to their ability to osseointegrate as a promising material [7,8]. In addition, zirconia-based materials have been claimed as a biomaterial with high chemical stability and metal-free that avoid toxic products release to the surrounding tissues [9,10].…”

Section: Introductionmentioning

confidence: 99%

Effects of surface topography through laser texturing on the surface characteristics of zirconia-based dental materials: surface hydrophobicity, antibacterial behavior, and cellular response

Ghalandarzadeh

Ganjali

Hosseini

2023

Surf. Topogr.: Metrol. Prop.

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The continuous need for high-performance implants that provide significant biological properties has led to extensive research into the topographic patterns of bioceramics in recent years. Their excellent aesthetics, biocompatibility, low plaque affinity, and ability to reproduce a natural-looking appearance have contributed to their success in dentistry. 3 mol% Yttria-stabilized zirconia (3YSZ) is gaining popularity as a material for dental implants due to its excellent mechanical properties and minimal degradation when exposed to body temperature. However, such materials show limited biological and antibacterial performance for dental applications. The purpose of this work was to develop microtopographies on the surface of 3YSZ ceramic by laser ablation technique, in order to improve its biological response and antibacterial behaviors. Two types of microtextures, including micro-grooves and micro-channels geometries were fabricated onto the zirconia ceramics using the laser ablation technique. The effects of different microtextures on the wettability, biological and antibacterial behaviors of 3YSZ ceramics were studied. The results indicate that all of the microstructure patterns are capable of improving the performance of 3YSZ. Wettability is a decisive factor that determines the antibacterial performance of textured zirconia ceramics. The microtextured surfaces all display hydrophobic behavior, thus yielding an effective improvement of antibacterial performance for 3YSZ ceramics. Cell-surface interactions were assessed for 7 days on both zirconia textured surfaces and a nontextured control with pre-osteoblast MC3T3-E1 cells. The obtained results showed the positive influence of textured zirconia surfaces on cell biological response.

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Tackling the challenges facing the clinical applications of pure PEO hydroxyapatite layers: Co-deposition of YSZ nanoparticles

Cited by 20 publications

References 75 publications

Combined Effects of HA Concentration and Unit Cell Geometry on the Biomechanical Behavior of PCL/HA Scaffold for Tissue Engineering Applications Produced by LPBF

Combined Effects of HA Concentration and Unit Cell Geometry on the Biomechanical Behavior of PCL/HA Scaffold for Tissue Engineering Applications Produced by LPBF

Tailoring zirconia surface topography via femtosecond laser-induced nanoscale features: effects on osteoblast cells and antibacterial properties

Effects of surface topography through laser texturing on the surface characteristics of zirconia-based dental materials: surface hydrophobicity, antibacterial behavior, and cellular response

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