Tackling the chemical diversity of microbial nonulosonic acids – a universal large-scale survey approach

Kleikamp, Hugo B.C.; Lin, Yue; McMillan, Duncan G. G.; Geelhoed, Jeanine S.; Naus-Wiezer, Suzanne N. H.; Baarlen, Peter van; Saha, Chinmoy; Louwen, Rogier; Sorokin, Dimitry Y.; Loosdrecht, Mark C.M. van; Pabst, Martin

doi:10.1039/c9sc06406k

Cited by 22 publications

(35 citation statements)

References 30 publications

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“…The production of NulOs by "Ca. Accumulibacter" was confirmed by lectin staining, the recent mass spectrometry survey (Kleikamp et al 2020a) and the phylogenetic analysis. To understand also the metabolism of NulOs, the proteome of the "Ca.…”

Section: Phylogenetic Analysismentioning

confidence: 84%

“…Accumulibacter", a non-pathogenic bacterium that was cultivated in a bioreactor without any host-pathogen interaction, were found to produce different types of NulOs. Moreover, these compounds were present in most bacteria and archaea recently tested by Kleikamp et al (Kleikamp et al 2020a). Thus, the synthesis of NulOs is not necessarily connected with the host-pathogen interaction.…”

Section: Importance Of Sialic Acids and Other Nonulosonic Acids In Nomentioning

confidence: 99%

“…1A) (Knirel et al 2003). These NulOs have recently been described as ubiquitous in the microbial world (Lewis et al 2009; Kleikamp et al 2020a), making their study and understanding highly relevant.…”

Section: Introductionmentioning

confidence: 99%

“…Regardless of the intensive studies of NulOs in animal tissue and on the surface-related structure of pathogenic bacteria cells, the presence, production and function of NulOs in non-pathogenic bacteria have not been widely realized and studied. Only very recently, a genome level study (Lewis et al 2009) and a NulOs universal survey by high resolution mass spectrometry (Kleikamp et al 2020a) revealed the unexpectedly wide distribution of nonulosonic acid biosynthesis (NAB) pathway genes and wide-spread occurrence of NulOs in non-pathogenic bacteria. These discoveries indicate that NulOs must be an important component in the EPS of bacterial aggregates in natural and engineered ecosystems, which has been completely overlooked at present.…”

Section: Introductionmentioning

confidence: 99%

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Production of nonulosonic acids in the extracellular polymeric substances of “CandidatusAccumulibacter phosphatis”

Tomás-Martínez

Kleikamp

Neu

et al. 2020

Preprint

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Nonulosonic acids (NulOs) are a family of acidic carbohydrates with a nine-carbon backbone, which include different related structures, such as sialic acids. They have mainly been studied for their relevance in animal cells and pathogenic bacteria. Recently, sialic acids have been discovered as important compound in the extracellular matrix of virtually all microbial life and in Candidatus Accumulibacter phosphatis, a well-studied polyphosphate-accumulating organism, in particular. Here, bioaggregates highly enriched with these bacteria (approx. 95% based on proteomic data) were used to study the production of NulOs in an enrichment of this microorganism. Fluorescence lectin-binding analysis, enzymatic quantification, and mass spectrometry were used to analyze the different NulOs present, showing a wide distribution and variety of these carbohydrates, such as sialic acids and bacterial NulOs, in the bioaggregates. Phylogenetic analysis confirmed the potential of Ca. Accumulibacter to produce different types of NulOs. Proteomic analysis showed the ability of Ca. Accumulibacter to reutilize and reincorporate these carbohydrates. This investigation points out the importance of diverse NulOs in non-pathogenic bacteria, which are normally overlooked. Sialic acids and other NulOs should be further investigated for their role in the ecology of Ca. Accumulibacter in particular, and biofilms in general.

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Section: Phylogenetic Analysismentioning

confidence: 84%

Section: Importance Of Sialic Acids and Other Nonulosonic Acids In Nomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Production of nonulosonic acids in the extracellular polymeric substances of “CandidatusAccumulibacter phosphatis”

Tomás-Martínez

Kleikamp

Neu

et al. 2020

Preprint

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“…4 ). While sialic acids in bacteria were previously associated exclusively with pathogens in immune response avoidance (Carlin et al 2009 ), recent research shows far more diversity within this class of sugars (Kleikamp et al 2020 ). Intriguingly, C. thermarum TA2.A1 also has ccmL and ccmM (Fig.…”

Section: Resultsmentioning

confidence: 99%

Genomic analysis of Caldalkalibacillus thermarum TA2.A1 reveals aerobic alkaliphilic metabolism and evolutionary hallmarks linking alkaliphilic bacteria and plant life

et al. 2020

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The aerobic thermoalkaliphile Caldalkalibacillus thermarum strain TA2.A1 is a member of a separate order of alkaliphilic bacteria closely related to the Bacillales order. Efforts to relate the genomic information of this evolutionary ancient organism to environmental adaptation have been thwarted by the inability to construct a complete genome. The existing draft genome is highly fragmented due to repetitive regions, and gaps between and over repetitive regions were unbridgeable. To address this, Oxford Nanopore Technology’s MinION allowed us to span these repeats through long reads, with over 6000-fold coverage. This resulted in a single 3.34 Mb circular chromosome. The profile of transporters and central metabolism gives insight into why the organism prefers glutamate over sucrose as carbon source. We propose that the deamination of glutamate allows alkalization of the immediate environment, an excellent example of how an extremophile modulates environmental conditions to suit its own requirements. Curiously, plant-like hallmark electron transfer enzymes and transporters are found throughout the genome, such as a cytochrome b6c1 complex and a CO2-concentrating transporter. In addition, multiple self-splicing group II intron-encoded proteins closely aligning to those of a telomerase reverse transcriptase in Arabidopsis thaliana were revealed. Collectively, these features suggest an evolutionary relationship to plant life.

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Concluding Remarks and Outlook

Weissbrodt

2024

Springer Theses

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Tackling the chemical diversity of microbial nonulosonic acids – a universal large-scale survey approach

Abstract: A novel large-scale survey approach for microbial nonulosonic acids (sialic acids) including a first molecular level comparative study is presented.

Cited by 22 publications

References 30 publications

Production of nonulosonic acids in the extracellular polymeric substances of “CandidatusAccumulibacter phosphatis”

Production of nonulosonic acids in the extracellular polymeric substances of “CandidatusAccumulibacter phosphatis”

Genomic analysis of Caldalkalibacillus thermarum TA2.A1 reveals aerobic alkaliphilic metabolism and evolutionary hallmarks linking alkaliphilic bacteria and plant life

Concluding Remarks and Outlook

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