Tacrolimus suppressed the production of cytokines involved in atopic dermatitis by direct stimulation of human PBMC system. (Comparison with steroids)

Sakuma, Shozo; Higashi, Yasuyuki; N, Sato; Sasakawa, Tatsuya; Sengoku, Takanori; Ohkubo, Y.; Amaya, Tadahiro; Goto, Toshio

doi:10.1016/s1567-5769(01)00059-5

Cited by 116 publications

(94 citation statements)

References 37 publications

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“…As a result, CaN fails to dephosphorylate the cytoplasmic component of the nuclear factor of activated T cells, and thereby the transport of nuclear factor of activated T cells to the nucleus, where they act as a transcription factor for several cytokine genes involved in the immune response and in inflammation (123). By its inhibitory effect on CaN, tacrolimus decreases the expression of IL-2, IL-3, IL-4, IL-5, interferon-gamma and granulocyte macrophage colony-stimulating factor, leading to a reduced inflammatory response (124). Studies in FKBP12 -/-knockout mice have shown that the FKBP12-FK506 complex is also affecting the expression of cell cycle proteins.…”

Section: Sirolimus Analoguesmentioning

confidence: 99%

The cell cycle: A critical therapeutic target to prevent vascular proliferative disease

Charron

Nili

Strauss

2006

Canadian Journal of Cardiology

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Section: Sirolimus Analoguesmentioning

confidence: 99%

The cell cycle: A critical therapeutic target to prevent vascular proliferative disease

Charron

Nili

Strauss

2006

Canadian Journal of Cardiology

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“…[5][6][7][8][9] Corticosteroids are well known to have potent anti-inflammatory effects, but topical use can cause intense skin atrophy, one of the serious side effects limiting their uses for chronic skin diseases. 5,7) Repeated application of corticosteroids on dorsal skin of rats also causes dramatic skin atrophy.…”

Section: -4)mentioning

confidence: 99%

“…FK-506 and cyclosporine A also exhibited side effects, such as severe nephrotoxicity and neurotoxicity. 6) Therefore, herbal medicines for clinical uses, such as Centella asiatica extract, Orengedokto and Sofusan, should be developed. 10,11) Nevertheless, antipsoriatic effects of these herbal medicines have not been thoroughly studied.…”

Section: -4)mentioning

confidence: 99%

“…[1][2][3][4] Cyclooxygenase (COX)-2 inhibitors non-steroidal anti-inflammatory drugs and corticosteroids and immunosuppressants FK-506 and cyclosporine A for Th1 cells have been used clinically for psoriasis. [5][6][7][8][9] Corticosteroids are well known to have potent anti-inflammatory effects, but topical use can cause intense skin atrophy, one of the serious side effects limiting their uses for chronic skin diseases.…”

mentioning

confidence: 99%

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Effect of Chunghyuldan in Chronic Oxazolone-Induced Mouse Dermatitis

Wee

Shin

Bae

et al. 2005

Biological & Pharmaceutical Bulletin

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Psoriasis is a chronic and inflammatory skin disorder. Psoriasis patients have been shown to have the bias of interferon (IFN)-g producing Th1 and cyclooxygenase (COX)-induced macrophage in lesion skin and peripheral blood. [1][2][3][4] Cyclooxygenase (COX)-2 inhibitors non-steroidal anti-inflammatory drugs and corticosteroids and immunosuppressants FK-506 and cyclosporine A for Th1 cells have been used clinically for psoriasis. [5][6][7][8][9] Corticosteroids are well known to have potent anti-inflammatory effects, but topical use can cause intense skin atrophy, one of the serious side effects limiting their uses for chronic skin diseases.5,7) Repeated application of corticosteroids on dorsal skin of rats also causes dramatic skin atrophy. FK-506 and cyclosporine A also exhibited side effects, such as severe nephrotoxicity and neurotoxicity. 6) Therefore, herbal medicines for clinical uses, such as Centella asiatica extract, Orengedokto and Sofusan, should be developed. 10,11) Nevertheless, antipsoriatic effects of these herbal medicines have not been thoroughly studied.During the screening program to discover such agents from herbal medicines, Chunghyuldan (CHD, Daio-Orengedokuto in Japanese) exhibited the anti-inflammatory effect. 12)Therefore, we evaluated the antipsoriatic effect of CHD in the oxazolone-induced mouse contact dermatitis model by topical administration and measured mRNA levels of COX-2 and some cytokines. CHD and metabolized CHD were prepared according to the previously reported methods of Cho et al. MATERIALS AND METHODS Materials 12)Animals The female BALB/c mice (20-25 g) were supplied from Orient Experimental Animal Breeding Center (Seoul, Korea). All animals were housed in wire cages at 20-22°C and 50Ϯ10% humidity, fed standard laboratory chow (Orient Experimental Animal Breeding Center, Seoul, Korea) and allowed water ad libitum. All procedures relating to animals and their care conformed to the international guidelines 'Principles of Laboratory Animals Care' (NIH publication no. 85-23, revised 1985).Contact Hypersensitivity An oxazolone-induced dermatitis was measured according to the previous method of Fujii et al.9) BALB/c mice were sensitized by application of 100 ml of 1.5% oxazolone in ethanol to the abdomen. Then a total of 20 ml of 1% oxazolone in a mixture of acetone and olive oil (4 : 1) was applied to both sides of the mouse ear every 3 d starting from 7 d after sensitization. Ear thickness was measured using a Digimatic Micrometer (Mitsutoyo Co., Tokyo, Japan) 72 h after each application of the oxazolone, test agents were applied in a total volume of 20 ml to both sides of the ear 30 min before and 3 h after each application of oxazolone RT-PCR Analysis Ear tissue extract for RT-PCR analysis were performed by the modified method of Chi et al. 13)Briefly, ears were excised 6 h after the last application of oxazolone, freezed in liquid nitrogen and homogenized by a mortar and pestle prechilled in liquid nitrogen. Total RNA was extracted by using TRI reagent according to the ma...

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“…be followed carefully. Dramatic skin atrophy is characteristic of corticosteroids upon repeated application on the dorsal skin of rats [8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

Untitled

2015

IJPSR

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Aim:The objective of this study was to evaluate the effect of Commiphora mukul on psoriasis. Materials and Methods: Six female Balb/C mice per group of which, the first three groups served as untreated, disease controls and standard (0.1% dexamethasone) respectively. Animals of groups 4, 5 and 6 were applied 0.5, 1 and 2% of the C. mukul extract on both the ears, respectively. Dermatitis was induced in mice by the application of oxazolone 1.5% (100 µL in ethanol) to the abdominal region for a period of six days. Starting seven days following sensitization, 20 µL of oxazolone 1% in a mixture of acetone and olive oil (4:1) was applied to both sides of the mouse ear on days 7, 10, 13 and 16. For detailed time-course analysis of ear swelling reactions, ear thickness was measured before sensitization phase (day 7) and after each elicitation on days 10, 13, 16 and 19. Results: C. mukul potently suppressed ear swelling at each time-point. The suppressive rates of C. mukul at concentrations of 0.5, 1 and 2% were 47.3, 55.4 and 62.2% on day 16, respectively as compared to the disease control. Microscopic examination revealed a relatively swollen ear in the disease model as compared to the control animals. Whereas condition was gradually improved in treated groups dose-dependently. Conclusion: The results suggest that C. mukul improves chronic inflammatory skin disorders.

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Tacrolimus suppressed the production of cytokines involved in atopic dermatitis by direct stimulation of human PBMC system. (Comparison with steroids)

Cited by 116 publications

References 37 publications

The cell cycle: A critical therapeutic target to prevent vascular proliferative disease

The cell cycle: A critical therapeutic target to prevent vascular proliferative disease

Effect of Chunghyuldan in Chronic Oxazolone-Induced Mouse Dermatitis

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