TaEPFL1, an EPIDERMAL PATTERNING FACTOR-LIKE (EPFL) secreted peptide gene, is required for stamen development in wheat

Sun, Qingxu; Qu, Junle; Yu, Yan; Yang, Zaijun; Wei, Shuhong; Wu, Yi-Hsin; Yang, Jun; Peng, Zhengsong

doi:10.1007/s10709-019-00061-7

Cited by 22 publications

(22 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genetic analysis in Arabidopsis demonstrated that four putative ligands (EPFL1, EPFL2, EPFL4, and EPFL6) function redundantly in the shoot apical meristem (SAM) by promoting organ elongation (Abrash et al, 2011;Uchida et al, 2012). The wheat gene TaEPFL1 is required for wheat stamen development (Sun et al, 2019). In our dataset, TaEPFL1 expression was high at the GA/YA stage (Supplemental Data Set 4), which is the main stage during which floral organ identity is established.…”

Section: Epidermal Patterning Factor-like (Epfl) Familymentioning

confidence: 76%

Ppd-1Remodels Spike Architecture by Regulating Floral Development in wheat

Liu

Zhang

Melzer

et al. 2020

Preprint

View full text Add to dashboard Cite

The determination of spike architecture is critical to grain yield in wheat (Triticum aestivum), yet the underlying mechanisms remain largely unknown. Here, we measured 51 traits associated with spike architecture and floral development in 197 wheat accessions with photoperiod sensitive and insensitive alleles. We included five distinct allele combinations at the Photoperiod-1 (Ppd-1) loci. A systematic dissection of all recorded phenotypes revealed connections between floral development, spike architecture and grain yield. Modifying the durations of spikelet primordia initiation did not necessarily affect spikelet number. In addition, Ppd-1 loci clearly influenced rachis dry weight, pointing to the rachis vascular system as a potential target for higher yield. Ppd-1 displayed opposite effects on the durations of pre and post-anthesis phases. Ppd-1 controlled carpel size, but not anther size. Finally, the photoperiod-insensitive alleles of Ppd-1 triggered floral degeneration. In parallel, we profiled the spike transcriptome at six stages and four positions in three Ppd-1 genotypes which consists of 234 samples. Integrating phenotypic and expression data suggested that loss of function in Ppd-1 loci delayed floral degeneration by regulating autophagy and extended floret development by regulating genes in different families. Therefore, we concluded that Ppd-1 remodels spike architecture by regulating floral development in wheat. Appleyard, 1987). During WA stage, the meristematic dome initiates 7-9 floret primordia within each individual spikelet (1-3 primordia fewer than the maximum number) (Kirby and Appleyard, 1987). The lemmas of florets 1 and 2 at the base of the spikelet completely enclose the anther and ovary (Kirby and Appleyard, 1987). At the GA stage, the meristematic dome completes the initiation of floret primordia, so that after this stage no more floret primordia will be produced ; the glumes almost completely cover all but the tips of the florets. The spike length peaks at the YA stage (Guo et al., 2018); glumes are fully formed, and the lemmas of the first three florets are visible. At the TP stage, the first awn is visible (Zadoks et al., 1974), at HD stage, half of the spike emerges from the last leaf blade (Zadoks et al., 1974). Finally, at the AN stage, yellow anthers appear along the spike (Zadoks et al., 1974).Spike architecture is a complicated trait, that is determined by multiple factors. Spike fertility index (i.e. the ratio between grain number per spike and weight of spike chaff) is a critical metric of assimilate distribution between grains and spike chaff (e.g. glume, lemma, palea, rachis). Grain number per spikelet along the main inflorescence also influences spike shape. Spikelet density (the ratio between spike length and spikelet number) also indicates spike compactness. Spikelet fertility (the ratio between fertile and total spikelet number) informs on the proportion of aborted spikelets. In addition, grain size traits (thousand-kernel weight-TKW, grain area, grain width, grain le...

show abstract

Section: Epidermal Patterning Factor-like (Epfl) Familymentioning

confidence: 76%

Ppd-1Remodels Spike Architecture by Regulating Floral Development in wheat

Liu

Zhang

Melzer

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…The identities and ratios between 123 DEG and 214 records are above 95% and 90%, respectively (Additional file 2: Table S2). stamens [13]. The alignment identity of TaEPFL1 gene against TraesCS6D02G296500.1 and TraesCS6B02G347500.1 were 100%.…”

Section: Discussionmentioning

confidence: 98%

“…In 2019, sun et al reported a TaEPFL1 gene, which was highly expressed in pistillody-stamens than in pistils and stamens. Heterologous expression of the TaEPFL1 gene in Arabidopsis caused shortened filaments and pedicels, which might induce stamens transformation into pistils or pistil-like structures in wheat [13]. Furthermore, some transcription factors of MADS-box and DROOPING LEAF gene of YABBY family have been considered to regulate in the development of wheat pistillody-stamen [6,9,[14][15][16][17][18].…”

mentioning

confidence: 99%

Co-expression network analysis of the genes associated with pistillody-stamen development in wheat

Liao¹,

Chen²,

Yang³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Crop male sterility has great values in both theoretical research and breeding application. Wheat pistillody-stamen is an important male sterility phenomenon, and HTS-1 is an important pistillody-stamen material. However the molecular mechanism of HTS-1 stamens transformed into pistils or pistil-like structures remains a mystery. Weighted gene co-expression network analysis (WGCNA) are widely used to explore hub genes and gene interaction networks from high throughput data in various plants. Results: In the present study, for exploring gene networks associated with wheat pistillody-stamen development, WGCNA was employed to analyze 11 RNA-sequencing (RNA-seq) data of wheat tissues, including stamens of CSTP, pistils and pistillody-stamen of HTS-1. 19 out of 25 merged modules were highly associated with specific wheat tissues, and the MEdarkseagreen1 module was highly related to wheat pistillody-stamen (correlation with weight r =0.7, correlation p-value p =0.02). Then 180 genes about wheat flower development were identified from the MEdarkseagreen1 module by GO term analysis. Among 180 genes, the hub gene number associated with anther, filament, style, and ovary development were 12, 3, 3, and 10, respectively. We compared the published pistillody related proteins with proteins of HTS-1 by BLAST. A total of 58 pistillody-stamen development associated proteins were validated by BLAST. MADS-box and YABBY transcription factor about pistillody-stamen development were also analyzed in wheat flower. There were 47 of MADS-box and 17 of YABBY transcription factors were identified. BLAST program was used to align the published pistillody associated MADS-box and YABBY transcription factors with transcription factors identified in wheat flower. Totally, 36 of 47 MADS-box and 14 of 17 YABBY transcription factors were considered to regulate the development of pistillody-stamen, which had never been reported yet. Conclusion: These results have systematically identified the key candidate genes about the development of HTS-1 substructures flower. The tissue-specific correlation network analyses provide important insights into the molecular interactions underlying psitillody-stamen development.

show abstract

“…The EPIDERMAL PATTERNING FACTOR-LIKE (EPFL) family has various functions in plant growth and development, such as guiding the inflorescence architecture and pedicel length. The TaEPFL1 gene encodes a secreted peptide with an essential role in stamen development in wheat [35]. Moreover, the TaEPFL1 gene is expressed at an abnormally high level in pistillody stamens compared with pistils and stamens.…”

Section: Degs Related To Pistillodymentioning

confidence: 99%

Comparative transcriptome analysis indicates conversion of stamens into pistil-like structures in male sterile wheat (Triticum aestivum L.) with Aegilops crassa cytoplasm

Liu

Wei

et al. 2020

BMC Genomics

View full text Add to dashboard Cite

Background: Aegilops crassa cytoplasm is an important source for investigating cytoplasmic male sterility (CMS). Moreover, the stamens of line C303A exhibit a high degree of pistillody, turning almost white. However, the molecular mechanism that underlies pistillody in C303A remains unclear. Therefore, to obtain a better understanding of pistillody in C303A, the phenotypic and cytological features of C303A were observed to identify the key stage for the homeotic transformation of stamens into pistil-like structures. Transcriptome profiles were determined for stamens using Illumina RNA sequencing. Results: Morphological observations of the CMS wheat line with Aegilops crassa cytoplasm C303A showed that the pistils developed normally, but the stamens were ultimately aborted and they released no pollen when mature. According to paraffin section observations, the stamens began to transform into pistils or pistil-like structures in the binucleate stage (BNS). Therefore, the stamens were collected from line C303A and its maintainer 303B in the BNS for transcriptome sequencing. In total, 20,444 wheat genes were determined as differentially expressed in C303A and 303B stamens, with 10,283 upregulated and 10,161 downregulated genes. Gene Ontology enrichment analyses showed that most of the differentially expressed genes (DEGs) were annotated with GO terms comprising metabolic process, cell, cellular process, catalytic activity, and cell part. Analysis based on the Kyoto Encyclopedia of Genes and Genomes database showed that the enriched DEGs were mainly associated with energy metabolism. We also found several essential genes that may contribute to pistillody in C303A. These findings suggest that disrupted energy metabolism and reactive oxygen metabolism induce pistillody and eventually lead to abortion in C303A. Conclusion: We determined the complex transcriptome profiles for C303A stamens and demonstrated that disrupted energy metabolism and class B MADS-box genes are related to pistillody. These findings may facilitate future studies of the mechanistic response of the wheat stamen and pollen development in CMS.

show abstract

TaEPFL1, an EPIDERMAL PATTERNING FACTOR-LIKE (EPFL) secreted peptide gene, is required for stamen development in wheat

Cited by 22 publications

References 36 publications

Ppd-1Remodels Spike Architecture by Regulating Floral Development in wheat

Ppd-1Remodels Spike Architecture by Regulating Floral Development in wheat

Co-expression network analysis of the genes associated with pistillody-stamen development in wheat

Comparative transcriptome analysis indicates conversion of stamens into pistil-like structures in male sterile wheat (Triticum aestivum L.) with Aegilops crassa cytoplasm

Contact Info

Product

Resources

About