Radio Frequency Identification (RFID) is a wireless technology that uses radio waves as a key for item tracking, localization of movable assets and for automatic operations. In some cases, it is not possible for the reader to identify all the tags which result in the tag being undetected. One of the factors that influence tag identification is interference. In RFID tag reading, data collisions often occur, therefore a method is needed to avoid data collisions, namely using the Slotted Aloha Method Under Interference Environment. In this paper discusses the calculation of distance calculations obtained using the RSSI technique and the speed of detection at the time of the interference and simulation of the detection at the time of the interference influence using the slotted aloha method. And the results obtained calculations results in the smallest distance difference is 0.00639 meters and the largest is 0.3877 meters, and the fastest average time when detecting 10 tags is when the interference probability is 0 which is 27.1828 ms and the longest average time when the detection of 10 tags is when the interference probability is 0.5 that is 54,3656 ms.