Studies were conducted to evaluate the toxicity of methanol (MeOH) extract from Ferula asafoetida Linn. (Umbelliferae) leaves on female adults and larvae of the lesser pumpkin fly, Dacus ciliatus (Diptera: Tephritidae). Direct-dip bioassays for larvae and topical bioassays for adults of fly were used to assess mortalities. Mortality increased significantly with rising concentrations. The intermediate lethal concentrations (LC50), calculated 7.3 mg/ml (7300 ppm) for larvae. The female adult LC50 was 409 µg/insect. The chemical composition of the isolated MeOH extract from Ferula asafoetida leaves was examined by gas chromatography-mass spectrometry. The major compounds were 2, 3, 4, 5-Tetramethyl thiophene (22.1%), Phenol, 3-pentadecyl- (21.2%) and Mono (2-ethylhexyl) phthalate (15.98%). The results suggested that the extract from the tested plant could be used as a potential control agent for this pest.