Tagging the next generation: validation of trans-generational chemical tagging for an endangered fish

Hobbs, James A.; Castillo, Gonzalo; Tigan, Galen; Lindberg, Joan C.; Ikemiyagi, Naoaki; Ramos, Georgia

doi:10.1007/s10641-012-0034-1

Cited by 12 publications

(7 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The advantage of using isotopes can be seen in the possibility of using significantly lower amounts of spiking material than an elemental approach (Hobbs et al 2012), reducing potential negative effects on the offspring. In this study, fertilisation, hatch success and juvenile survival rates were within the range of long-term records at the hatchery.…”

Section: Discussionmentioning

confidence: 99%

“…Kuroki et al 2010;Shippentower et al 2011;Hobbs et al 2012) used elemental Sr as a marker to reference the alteration of the Sr/Ca ratio in the core region of the otoliths. Another disadvantage of elemental transgenerational marking is that much larger doses are required to induce a measurable change, which may have negative effects on the condition and health of the offspring (Hobbs et al 2012). Another disadvantage of elemental transgenerational marking is that much larger doses are required to induce a measurable change, which may have negative effects on the condition and health of the offspring (Hobbs et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…These studies and others (e.g. Kuroki et al 2010;Shippentower et al 2011;Hobbs et al 2012) used elemental Sr as a marker to reference the alteration of the Sr/Ca ratio in the core region of the otoliths. However, the Sr/Ca ratio in otoliths may vary naturally (Shippentower et al 2011) thus marking might be masked.…”

Section: Introductionmentioning

confidence: 99%

“…However, the Sr/Ca ratio in otoliths may vary naturally (Shippentower et al 2011) thus marking might be masked. Another disadvantage of elemental transgenerational marking is that much larger doses are required to induce a measurable change, which may have negative effects on the condition and health of the offspring (Hobbs et al 2012).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Transgenerational marking of brown trout Salmo trutta f.f., using an ⁸⁴Sr spike

Zitek

Irrgeher

Kletzl³

et al. 2013

Fisheries Management Eco

View full text Add to dashboard Cite

The maternal transmission of a naturally occurring and non‐toxic minor isotope of strontium (84Sr) to the central otolith region of the progeny of a typical European freshwater fish species, brown trout, Salmo trutta f.f. L. was accomplished. The focus was to apply minimum doses of a non‐toxic solution at physiological salt concentration to minimise potential adverse effects on the fish. Female spawners were intraperitoneally injected with doses of 12.5 and 30.2 μg 84Sr kg−1 fish. Eggs were stripped and the resulting progeny were reared in a hatchery for about 1 year before sampling the otoliths. Strontium isotope ratios in the otoliths of the offspring were measured by cross‐sectional line scans using laser ablation‐multiple collector‐inductively coupled plasma mass spectrometry (LA‐MC‐ICPMS). Otolith cores of marked juveniles showed a significantly elevated 84Sr/86Sr ratio compared with control fish and to otolith regions created after hatching. Mass marking of cohorts of progeny from individually spiked brown trout with Sr isotopes is therefore possible for dispersal and migration studies without the necessity of handling eggs and applying other larval marking methods like immersion.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transgenerational marking of brown trout Salmo trutta f.f., using an ⁸⁴Sr spike

Zitek

Irrgeher

Kletzl³

et al. 2013

Fisheries Management Eco

View full text Add to dashboard Cite

show abstract

“…a spike solution with high concentration of an element) lies in the fact that the elemental concentrations in otoliths may vary naturally to such an extent as to prevent an unambiguous marking (Shippentower et al 2011), whereas single isotopic spikes can create signatures exceeding the possible natural range (Munro et al 2009). In addition, elemental transgenerational marking will likely require much larger dosages to induce a measurable change, which could cause negative effects on the condition and health of the offspring (Hobbs et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Individual-specific transgenerational marking of common carp Cyprinus carpio, L., using 86Sr/84Sr double spikes

Zitek

Irrgeher

Cervicek

et al. 2014

Mar. Freshwater Res.

View full text Add to dashboard Cite

Transgenerational isotopic marking has been recognised as an efficient tool for mass marking of high numbers of fish larvae by injecting female spawners with enriched isotope solutions. So far, mainly enriched stable barium isotopes have been applied for this purpose. Here, we present an alternative approach for individual-specific transgenerational marking using strontium 86 Sr/ 84 Sr double spikes. Four isotonic double-spike solutions with different molar fractions of 86 Sr and 84 Sr and different total Sr concentrations were administered to four female spawners of common carp, Cyprinus carpio, L., by intraperitoneal injection, and one additional female spawner was injected a blank isotonic solution as control. Otoliths (lapilli) were sampled from juvenile offspring and analysed for their Sr isotopic composition by laser ablation-multi collector-inductively coupled plasma-mass spectrometry. Central otolith regions of the progeny of female carps treated with concentrations of at least 0.45 mg 84 Sr kg À1 bodyweight and 2.28 mg 86 Sr kg À1 bodyweight showed a significant shift of the absolute 88 Sr/ 86 Sr and 88 Sr/ 84 Sr isotope ratios from the natural baseline. Isotope pattern deconvolution was successfully applied for the identification of the originally injected 86 Sr/ 84 Sr molar fraction ratios of the individual double spikes. Enriched stable Sr isotope double spikes represent an important alternative to enriched stable Ba isotopes for transgenerational marking, especially in freshwater systems.

show abstract