Tail nerve electrical stimulation promoted the efficiency of transplanted spinal cord-like tissue as a neuronal relay to repair the motor function of rats with transected spinal cord injury

Multifunctional Hierarchical Nanoplatform with Anisotropic Bimodal Mesopores for Effective Neural Circuit Reconstruction after Spinal Cord Injury

“…40,41 Demyelination involves the degradation of myelin proteins and membranes surrounding neuronal axons, while prompt remyelination contributes to functional neurological recovery after SCI. 42,43 Luxol fast blue (LFB) staining revealed a noteworthy reduction in the LFB-positive area at the injury center on day 28 post SCI, suggesting the persistent demyelination response (Figure 7e synaptic reconstruction across the injured region and subsequently facilitated motor function recovery following SCI. We believe that this kind of dual-mesoporous nanoplatform, capable of dual drugs delivery, holds great potential for treating complex diseases.…”

“…Furthermore, the lesion area was determined based on the boundary of the glial scar, and it is discovered that treatment with Janus mSiO 2 &PMO-CeO 2 –PTX&ENO nanoformulation decreased the damaged area by approximately 60% (Figure d). Then, we assessed myelin sheath generation after a different treatment; these sheaths are responsible for enveloping neuronal axons and facilitating the rapid propagation of electrical impulses along these axonal conduits. , Demyelination involves the degradation of myelin proteins and membranes surrounding neuronal axons, while prompt remyelination contributes to functional neurological recovery after SCI. , Luxol fast blue (LFB) staining revealed a noteworthy reduction in the LFB-positive area at the injury center on day 28 post SCI, suggesting the persistent demyelination response (Figure e,f). The administration of mSiO 2 &PMO-CeO 2 –PTX&ENO effectively increased the LFB-positive region while reducing the area of the lesion site compared to the mSiO 2 –CeO 2 –PTX+ENO and PMO-CeO 2 –PTX+ENO groups.…”

Combinatorial strategies for cell transplantation in traumatic spinal cord injury

“…The authors found that the combination of nerve stimulation and transplantation resulted in the greatest locomotor functional recovery compared to SCI only, transplant only, and stimulation only groups, with animals in the combined treatment group achieving weight-supported stepping. These subjects also exhibited increased regeneration of neurofilament + axons and myelination of regenerating axons, compared to other groups; additionally, transsynaptic tracing results also demonstrated a greater degree of neural connectivity across the lesion site in animals with combined treatment (Lai et al, 2023). applied physiological electric field stimulation to NSCs prior to transplantation, in order to mimic the electrical potential that is present in the developing neural tube as a method to promote survival and differentiation.…”

“…To date, only a handful of studies have examined the effects of neuromodulation on NSPC engraftment and associated functional recovery after transplantation into sites of SCI. Recently, Lai et al (2023) performed electrical stimulation of the tail nerve as a neuromodulatory approach to promote conduction of signals in neural stem cell-derived “spinal cord-like tissue” transplants in sites of complete spinal cord transection. Adult hippocampal rat NSCs expressing the neurotrophic factor NT-3 and receptor TrkC were cultured together with oligodendrocyte precursor cells expressing CNTF in collagen scaffolds to form the spinal cord-like tissue transplants, which were placed into sites of T10 transection SCI immediately following injury.…”

Functional biomaterials for modulating the dysfunctional pathological microenvironment of spinal cord injury

“…However, spinal cord-like tissue module always lacks properties of gray matter-like tissue, which contains a variety of nerve cells in different sizes, shapes, and functions to receive and emit impulses. Hence, a spinal cord-like tissue incorporated both WMLT (including CNTF-overexpression oligodendrocytes) and gray matter-like tissue (GMLT) (including NT-3/TrkC-overexpression neurons) was fabricated recently using collagen sponge scaffold [ 167 ]. Transplantation of this spinal cord-like tissue into SCI rats enhanced their neuronal regrowth and remyelination, also improved microenvironment of muscle tissue, finally promoting functional recovery.…”