Tailorable polyelectrolyte protein complex based on poly(aspartic acid) and bovine serum albumin

Niţă, Loredana E.; Chiriac, Aurica P.; Stoleru, Elena; Diaconu, Alina; Tudorachi, Niță

doi:10.1080/15685551.2016.1187436

Cited by 12 publications

(10 citation statements)

References 25 publications

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“…In the 7:1-5:1 mass ratio interval, the increased MAC mass content led to the condensation of denatured BSA molecules. When increasing the ratio of one polyelectrolyte to the other one, as already observed by other researchers, many coacervates typically reach a turbidity maximum, followed by a sudden loss in optical transmittance concretized in even a completely transparent polymer solution (5:1 ratio) [28][29][30].…”

Section: Turbidity Measurements Of Nanogel Solutionssupporting

confidence: 69%

Self-Assembled Nanocarriers Based on Modified Chitosan for Biomedical Applications: Preparation and Characterization

Rusu¹,

Chiriac²,

Niţă³

et al. 2020

Polymers

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Protein–polysaccharide systems are of increasing interest as their combined attributes allow for fulfilling a broad range of applications in biomedical and pharmaceutical fields. In this study, the preparation of nanogels based on maleic anhydride chitosan derivatives (MAC) and bovine serum albumin (BSA) was achieved through a self-assembly process performed in aqueous phase. A series of experiments performed by varying the concentrations of MAC and BSA were conducted to find an appropriate mixing ratio for the polymer solutions leading to thermodynamically stable nanogels with the ability to encapsulate active compounds. The influence of temperature on the formation of nanogels was also studied. The consequent conformational changes were monitored using ultraviolet–visible (UV-VIS) spectrophotometry. The spectrophotometric investigations combined with diffraction light scattering (DLS) technique and zeta potential measurement results were correlated to determine the interaction mechanism and assess the self-assembling processes during nanogel formation. It was found that the hydrodynamic diameter (Dh) of the nanoparticles increased slightly at acidic pH, and the protonation of ionizable amino groups with the pH was confirmed by the zeta potential measurements. MAC/BSA nanogels also exhibited antimicrobial properties after being loaded with amoxicillin (Amox), which is an antibiotic used for the treatment of various infections. The experimental data resulting from this study provide theoretical guidance for the design and development of attractive nanocarriers for a large variety of biomedical applications.

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Section: Turbidity Measurements Of Nanogel Solutionssupporting

confidence: 69%

Self-Assembled Nanocarriers Based on Modified Chitosan for Biomedical Applications: Preparation and Characterization

Rusu¹,

Chiriac²,

Niţă³

et al. 2020

Polymers

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“…This means that when PLA/CS/R samples come in contact with blood, they cause an increase in the work of cohesion for platelets, therefore the aforementioned blood cells will not adhere easily onto surface of biomaterial, avoiding occurrence of thrombosis, which is an unwanted event for blood-contacting applications. [119,120]…”

Section: Resultsmentioning

confidence: 99%

Biocompatible Materials Based on Plasticized Poly(lactic acid), Chitosan and Rosemary Ethanolic Extract I. Effect of Chitosan on the Properties of Plasticized Poly(lactic acid) Materials

et al. 2019

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The purpose of the present study is to develop new multifunctional environmentally friendly materials having applications both in medical and food packaging fields. New poly(lactic acid) (PLA)-based multifunctional materials containing additives derived from natural resources like chitosan (CS) and rosemary extract (R) were obtained by melt mixing. Each of the selected components has its own specific properties such as: PLA is a biodegradable thermoplastic aliphatic polyester derived from renewable biomass, heat-resistant, with mechanical properties close to those of polystyrene and polyethylene terephthalate, and CS offers good antimicrobial activity and biological functions, while R significantly improves antioxidative action necessary in all applications. A synergy of their combination, an optimum choice of their ratio, and processing parameters led to high performance antimicrobial/antioxidant/biocompatible/environmentally degradable materials. The polyethylene glycol (PEG)-plasticized PLA/chitosan/powdered rosemary extract biocomposites of various compositions were characterized in respect to their mechanical and rheological properties, structure by spectroscopy, antioxidant and antimicrobial activities, and in vitro and in vivo biocompatibility. Scanning electron microscopy images evidence the morphology features added by rosemary powder presence in polymeric materials. Incorporation of additives improved elongation at break, antibacterial and antioxidant activity and also biocompatibility. Migration of bioactive components into D1 simulant is slower for PEG-plasticized PLA containing 6 wt % chitosan and 0.5 wt % rosemary extract (PLA/PEG/6CS/0.5 R) biocomposite and it occurred by a diffusion-controlled mechanism. The biocomposites show high hydrophilicity and good in vitro and in vivo biocompatibility. No hematological, biochemical and immunological modifications are induced by subcutaneous implantation of biocomposites. All characteristics of the PEG-plasticized PLA-based biocomposites recommend them as valuable materials for biomedical implants, and as well as for the design of innovative drug delivery systems. Also, the developed biocomposites could be a potential nature-derived active packaging with controlled release of antimicrobial/antioxidant compounds.

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“…As revealed in Table 11 , the work of spreading of the red blood cells, W s/rbc , has positive values (except for the PLA and PLA/0.5R samples) and the work of the spreading of platelets, W s/p , has negative values, which suggests that the work of adhesion is higher than that of cohesion for the red blood cells, but, comparatively, a smaller work of adhesion than that of cohesion for platelets. These findings denote that the contact of blood with PLA/R-based composites causes an increase in the work of cohesion for platelets; hence the platelets will not adhere easily onto the biomaterial’s surface, thus avoiding the appearance of thrombosis [ 87 ].…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the Rosemary Extract Effect on the Properties of Polylactic Acid-Based Materials

Darie-Niță¹,

Vasile²,

Stoleru³

et al. 2018

Materials

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New multifunctional materials containing additives derived from natural resources as powdered rosemary ethanolic extract were obtained by melt mixing and processed in good conditions without degradation and loss of additives. Incorporation of powdered rosemary ethanolic extract (R) into poly(lactic acid) (PLA) improved elongation at break, rheological properties, antibacterial and antioxidant activities, in addition to the biocompatibility. The good accordance between results of the chemiluminescence method and radical scavenging activity determination by chemical method evidenced the increased thermoxidative stability of the PLA biocomposites with respect to neat PLA, with R acting as an antioxidant. PLA/R biocomposites also showed low permeability to gases and migration rates of the bioactive compounds and could be considered as high-performance materials for food packaging. In vitro biocompatibility based on the determination of surface properties demonstrated a good hydrophilicity, better spreading and division of fibroblasts, and increased platelet cohesion. The implantation of PLA/R pellets, was proven to possess a good in vivo biocompatibility, and resulted in similar changes in blood parameters and biochemical responses with the control group, suggesting that these PLA-based materials demonstrate very desirable properties as potential biomaterials, useful in human medicine for tissue engineering, wound management, orthopedic devices, scaffolds, drug delivery systems, etc. Therefore, PLA/R-based materials show promising properties for applications both in food packaging and as bioactive biomaterials.

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Tailorable polyelectrolyte protein complex based on poly(aspartic acid) and bovine serum albumin

Cited by 12 publications

References 25 publications

Self-Assembled Nanocarriers Based on Modified Chitosan for Biomedical Applications: Preparation and Characterization

Self-Assembled Nanocarriers Based on Modified Chitosan for Biomedical Applications: Preparation and Characterization

Biocompatible Materials Based on Plasticized Poly(lactic acid), Chitosan and Rosemary Ethanolic Extract I. Effect of Chitosan on the Properties of Plasticized Poly(lactic acid) Materials

Evaluation of the Rosemary Extract Effect on the Properties of Polylactic Acid-Based Materials

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