Tailored adsorption of His<sub>6</sub>-tagged protein onto nickel(ii)–cyclam grafted mesoporous silica

Gaffney, Darragh; O‘Neill, Sarah; O’Loughlin, Mary C.; Hanefeld, Ulf; Cooney, Jakki C.; Magner, Edmond

doi:10.1039/b915169a

Cited by 31 publications

(20 citation statements)

References 28 publications

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“…Tailoring of the surface functional groups of MPS and of a protein can enable anchoring of the protein so that binding occurs in a specific manner, thus ensuring that the enzyme is not only irreversibly bound but also in an orientation which is substrate accessible. Such an approach has been described recently by anchoring an enzyme to MPS via a histidine tag which can coordinate to metal ions on the surface of MPS [42,43].…”

Section: Immobilization Of Enzymes By Covalent Methodsmentioning

confidence: 99%

“…However, the oxide moiety is not conducive to the coordination of ligands, which is necessary for the attachment of proteins. The third option, using a spacer ligand to post-synthetically tether the organometallic complex on the silicate surface, has previously been employed with some success [20,22,38] This has been demonstrated by the attachment of N-(trimethoxysilylpropyl) EDTA (ethylene diamine triacetic acid) to the surface of the silicate and subsequent use as a tethering ligand for nickel [42,43]. The main advantage of this method is that the oxidation state of the coordinated metal ion is retained.…”

Section: Metal Functionalized Mpsmentioning

confidence: 99%

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Modification of Mesoporous Silicates for Immobilization of Enzymes

2012

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Mesoporous silicates (MPS) possess ordered pore structures with pore diameters sufficiently large to accommodate a range of enzymes. The successful immobilization of an enzyme on MPS usually requires modification of the surface of MPS to optimize the interactions between the support and the enzyme. Recent developments on the 2 functionalization of MPS for the immobilization of enzymes are described in this review.

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Section: Immobilization Of Enzymes By Covalent Methodsmentioning

confidence: 99%

Section: Metal Functionalized Mpsmentioning

confidence: 99%

Modification of Mesoporous Silicates for Immobilization of Enzymes

2012

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“…The preparation of a His-tagged protein on a Ni functionalised MPS was demonstrated using a protease inhibitor (Spi) from Streptococcus pyogenes 110 (Fig.7). 41 The His 6 -tagged form of Spi bears a low overall charge ensuring …”

Section: Covalent Immobilisation Of Enzymesmentioning

confidence: 99%

Immobilisation of enzymes on mesoporous silicate materials

2013

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Mesoproous silicates (MPS) are attractive materials for the immobilisation of enzymes. They possess 5 ordered pore structures, narrow pore size distributions, large surface areas, high stability and can be chemically modified with various functional groups. The properties of MPS materials are reviewed in terms of their ability to act as supports for enzymes for use in biocatalysis with a particular focus on the ability to tailor the surface functionalization of the MPS to suit a specific enzyme. While many reports of the immobilisation of enzymes on MPS have been described, their use as biocatalytic supports is limited. 10Large scale reactors based on MPS will require continuous flow systems where the properties of the support can be tailored while allowing fluid flow at reasonable low pressure.

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“…Consequently, affinity binding showed a higher adsorption capacity (98.1 mg g 21 particles) and activity recovery (92.5%) than the physical adsorption procedure. Cu-and Ni-modified silicas were also used to specifically bind His-tagged lipase [85,86] and a protease inhibitor [87]. Another immobilization technique is to form a spacer arm between the enzyme and activated matrix [88].…”

Section: Silica Nanocarriersmentioning

confidence: 99%

Nanobiocatalyst advancements and bioprocessing applications

Misson

Zhang

Jin

2015

J. R. Soc. Interface.

215

108

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The nanobiocatalyst (NBC) is an emerging innovation that synergistically integrates advanced nanotechnology with biotechnology and promises exciting advantages for improving enzyme activity, stability, capability and engineering performances in bioprocessing applications. NBCs are fabricated by immobilizing enzymes with functional nanomaterials as enzyme carriers or containers. In this paper, we review the recent developments of novel nanocarriers/nanocontainers with advanced hierarchical porous structures for retaining enzymes, such as nanofibres (NFs), mesoporous nanocarriers and nanocages. Strategies for immobilizing enzymes onto nanocarriers made from polymers, silicas, carbons and metals by physical adsorption, covalent binding, cross-linking or specific ligand spacers are discussed. The resulting NBCs are critically evaluated in terms of their bioprocessing performances. Excellent performances are demonstrated through enhanced NBC catalytic activity and stability due to conformational changes upon immobilization and localized nanoenvironments, and NBC reutilization by assembling magnetic nanoparticles into NBCs to defray the high operational costs associated with enzyme production and nanocarrier synthesis. We also highlight several challenges associated with the NBC-driven bioprocess applications, including the maturation of large-scale nanocarrier synthesis, design and development of bioreactors to accommodate NBCs, and long-term operations of NBCs. We suggest these challenges are to be addressed through joint collaboration of chemists, engineers and material scientists. Finally, we have demonstrated the great potential of NBCs in manufacturing bioprocesses in the near future through successful laboratory trials of NBCs in carbohydrate hydrolysis, biofuel production and biotransformation.

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Tailored adsorption of His₆-tagged protein onto nickel(ii)–cyclam grafted mesoporous silica

Abstract: The specific immobilisation of a histidine-tagged protein, Spi, onto mono-anchored nickel(II)-cyclam functionalised SBA-15 is reported.Scheme 1 Surface modification of SBA-15 with Ni-cyclam and subsequent attachment of a His 6 -tagged protein.

Cited by 31 publications

References 28 publications

Modification of Mesoporous Silicates for Immobilization of Enzymes

Modification of Mesoporous Silicates for Immobilization of Enzymes

Immobilisation of enzymes on mesoporous silicate materials

Nanobiocatalyst advancements and bioprocessing applications

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Tailored adsorption of His6-tagged protein onto nickel(ii)–cyclam grafted mesoporous silica

Abstract: The specific immobilisation of a histidine-tagged protein, Spi, onto mono-anchored nickel(II)-cyclam functionalised SBA-15 is reported.Scheme 1 Surface modification of SBA-15 with Ni-cyclam and subsequent attachment of a His 6 -tagged protein.

Cited by 31 publications

References 28 publications

Modification of Mesoporous Silicates for Immobilization of Enzymes

Modification of Mesoporous Silicates for Immobilization of Enzymes

Immobilisation of enzymes on mesoporous silicate materials

Nanobiocatalyst advancements and bioprocessing applications

Contact Info

Product

Resources

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Tailored adsorption of His₆-tagged protein onto nickel(ii)–cyclam grafted mesoporous silica