Tailored light sheets through opaque cylindrical lenses

Battista, Diego Di; Ancora, Daniele; Zhang, Haisu; Lemonaki, Krystalia; Marakis, Evangelos; Liapis, Evangelos; Tzortzakis, S.; Zacharakis, Giannis

doi:10.1364/optica.3.001237

Cited by 24 publications

(17 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This field is known as the “speckle pattern” due to its grainy appearance and, for imaging applications, it is usually considered detrimental as it adds up to the features of interest [8]. In some applications, and when wavefront shaping is applied, the speckle pattern can be exploited to overcome scattering and diffusion in opaque samples, but not without limitations in terms of complexity and general applicability [9,10].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Light Sheet Elastic Scattering Microscopy by Using a Supercontinuum Laser

Battista

Merino

Zacharakis

et al. 2019

MPs

Self Cite

View full text Add to dashboard Cite

Light sheet fluorescence microscopy techniques have revolutionized biological microscopy enabling low-phototoxic long-term 3D imaging of living samples. Although there exist many light sheet microscopy (LSM) implementations relying on fluorescence, just a few works have paid attention to the laser elastic scattering source of contrast available in every light sheet microscope. Interestingly, elastic scattering can potentially disclose valuable information from the structure and composition of the sample at different spatial scales. However, when coherent scattered light is detected with a camera sensor, a speckled intensity is generated on top of the native imaged features, compromising their visibility. In this work, we propose a novel light sheet based optical setup which implements three strategies for dealing with speckles of elastic scattering images: (i) polarization filtering; (ii) reducing the temporal coherence of the excitation laser light; and, (iii) reducing the spatial coherence of the light sheet. Finally, we show how these strategies enable pristine light-sheet elastic-scattering imaging of structural features in challenging biological samples avoiding the deleterious effects of speckle, and without relying on, but complementing, fluorescent labelling.

show abstract

Section: Introductionmentioning

confidence: 99%

Enhanced Light Sheet Elastic Scattering Microscopy by Using a Supercontinuum Laser

Battista

Merino

Zacharakis

et al. 2019

MPs

Self Cite

View full text Add to dashboard Cite

show abstract

“…All of this will result in a highly optimized light exposition in the sample. Finally, recent demonstrations take advantage of the natural scattering properties that tissues present for generating LSs directly in the biological sample [208]. All these, including the new possibilities given by the new degrees of freedom and its multiple configurations, will make LSFM a powerful imaging technique with endless imaging possibilities.…”

Section: Discussionmentioning

confidence: 99%

Light-sheet microscopy: a tutorial

et al. 2018

View full text Add to dashboard Cite

This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective. As such, emphasis is placed on the advantages that LS microscope configurations present, given the degree of freedom gained by uncoupling the excitation and detection arms. The new imaging properties are first highlighted in terms of optical parameters and how these have enabled several biomedical applications. Then, the basics are presented for understanding how a LS microscope works. This is followed by a presentation of a tutorial for LS microscope designs, each working at different resolutions and for different applications. Then, based on a numerical Fourier analysis and given the multiple possibilities for generating the LS in the microscope (using Gaussian, Bessel, and Airy beams in the linear and nonlinear regimes), a systematic comparison of their optical performance is presented. Finally, based on advances in optics and photonics, the novel optical implementations possible in a LS microscope are highlighted.

show abstract

“…In this case, the employed disordered medium must be fully characterized to understand its effect on an incident wavefront. In knowing the effect of the disordered medium, the distortion it introduces can be removed via post-processing or exploited in conjunction with a source to achieve more sophisticated functionalities [70][71][72][73]. Fig.…”

Section: Disorder-engineered Metasurfaces For Wavefront Shapingmentioning

confidence: 99%

Broadband transparent and CMOS-compatible flat optics with silicon nitride metasurfaces [Invited]

Colburn¹,

Zhan²,

Bayati³

et al. 2018

Opt. Mater. Express

View full text Add to dashboard Cite

Metasurface optics is a promising candidate for realizing the next generation of miniaturized optical components. Unlike refractive optics, these devices modify light over a wavelength-scale thickness, changing the phase, amplitude, and polarization. This review details recent developments and state-of-the-art metasurfaces realized using silicon nitride. We emphasize this material as to date it has the lowest refractive index with which metasurfaces have been experimentally demonstrated. The wide band gap of silicon nitride enables reduced absorption over a broad wavelength range relative to its higher index counterparts, providing a CMOS-compatible platform for producing a variety of high efficiency metasurface elements and systems.

show abstract

Tailored light sheets through opaque cylindrical lenses

Cited by 24 publications

References 28 publications

Enhanced Light Sheet Elastic Scattering Microscopy by Using a Supercontinuum Laser

Enhanced Light Sheet Elastic Scattering Microscopy by Using a Supercontinuum Laser

Light-sheet microscopy: a tutorial

Broadband transparent and CMOS-compatible flat optics with silicon nitride metasurfaces [Invited]

Contact Info

Product

Resources

About