Tailored monoclonal antibody as recognition probe of immunosensor for ultrasensitive detection of silk fibroin and use in the study of archaeological samples

Li, Jin; Ouyang, Yi; Liu, Linshuai; Zhu, Chengyu; Meng, Junjing; Zheng, Hailing; Zhou, Yang; Wan, Junmin; Hu, Zhiwen; Wang, Bing

doi:10.1016/j.bios.2019.111709

Cited by 16 publications

(3 citation statements)

References 29 publications

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“…The preparation of pure silk fibroin and monoclonal antibody was consistent with our previous work and described in the Supporting Information.…”

Section: Methodsmentioning

confidence: 65%

Ultrasensitive Electrochemical Immunosensor Reveals the Existence of Silk Products on the Maritime Silk Road

Zheng

et al. 2019

ACS Sens.

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The Maritime Silk Road was the major trade route between eastern and western civilizations in the Middle Ages. However, hardly any silk products have been found along the transoceanic trade route. Thus, the extrasensitive detection of silk relic traces has tremendous importance in research regarding the Maritime Silk Road. In this study, an electrochemical immunosensor based on a tailored monoclonal antibody and gold nanoparticles using the layer-by-layer self-assembly method was devised. The fabricated immunosensor demonstrated preeminent performance in the analysis of silk fibroin, with a linear detection range of 0.01−100 ng mL −1 and a detection limit of 3.8 pg mL −1 . In particular, the performance of the immunosensor was excellent in the analysis of ancient silk samples, especially in the qualitative and quantitative detection of soil samples extracted from Nanhai No. 1 shipwreck archeological sites. The proposed electrochemical immunosensor proves the existence of silk products on the Maritime Silk Road and demonstrates enormous potential for studying the formation and development of the ancient transoceanic trading route.

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“…The preparation of pure silk fibroin and monoclonal antibody was consistent with our previous work and described in the Supporting Information.…”

Section: Methodsmentioning

confidence: 65%

Ultrasensitive Electrochemical Immunosensor Reveals the Existence of Silk Products on the Maritime Silk Road

Zheng

et al. 2019

ACS Sens.

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“…[2][3][4] Along this road, cultural exchanges and collisions between the East and the West have promoted cultural and technological development. 5 Therefore, it is of great historical and cultural value to explore the ageing and preservation of silk relics, especially the origin of silk.…”

Section: Introductionmentioning

confidence: 99%

In situ detection of silk fibroin using a dual recognition strategy with a flexible pressure immunosensor

Deng,

Zhang,

et al. 2024

Anal. Methods

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“…Immunosensor [19], Immunochromatographic Strip [20,21], Immune-Fluorescence [22,23], and ELISA. Among these, ELISA has been frequently used in the detection of silk relics [22,[24][25][26]] and other proteinbased residues [27][28][29] because of its high speci city, high sensitivity, and relative rapidity.…”

Section: Introductionmentioning

confidence: 99%

Multi-faceted Analysis Reveals the Characteristics of Silk Fabrics on A Liao Dynasty DieXie Belt

Ge,

Cong,

et al. 2023

Preprint

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The Liao dynasty's highly developed textile industry was characterized as "the best in the world" in ancient Chinese literature. This study analyzed two textiles on a 蹀躞(DieXie)belt excavated from the No.1 Liao dynasty noble tomb of Zhangjiayao Forest (Shenyang, China), with one wrapping around the leather belt (T1) and the other on the surface of a leather pouch hanging on the belt (X1). They were identified as silk based on structures by morphological observation and chemical components revealed by Fourier Transform Infrared spectroscopy (FTIR). Proteomics and enzyme-linked immunosorbent assay (ELISA) were used to investigate the animal origin of the silk, and the original color and dyestuffs of T1 were examined by liquid chromatography-mass spectrometer (LC-MS/MS). The results have shown that T1 consists of three layers of Bombyx mori silk plain fabric, and its original color was yellow-red dyed with madder and plants containing flavonoid dyeing tissue, consistent with the color of DieXie belts recorded in the ancient literature. The silk on the pouch is a layer of plain fabric, while the upper layer is embroidery on the Four-end-complex gauze, which was made of twisted Bombyx mori silk. We believe that the silk wrapping around the leather belt was to provide additional comfort and aesthetics after dyeing while protecting the leather, which is prone to warping and moisture. This study suggests that such silk fabrics with high specifications are one of the manifestations of the distinguished status of the tomb owner. The integration of various analytical methods on ancient silk fabrics enables us to learn various information about their textile forms, silk animal origins, colors and dyestuffs, revealing the highly developed silk waving techniques and prosperous costume culture of the Liao Dynasty.

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Tailored monoclonal antibody as recognition probe of immunosensor for ultrasensitive detection of silk fibroin and use in the study of archaeological samples

Cited by 16 publications

References 29 publications

Ultrasensitive Electrochemical Immunosensor Reveals the Existence of Silk Products on the Maritime Silk Road

Ultrasensitive Electrochemical Immunosensor Reveals the Existence of Silk Products on the Maritime Silk Road

In situ detection of silk fibroin using a dual recognition strategy with a flexible pressure immunosensor

Multi-faceted Analysis Reveals the Characteristics of Silk Fabrics on A Liao Dynasty DieXie Belt

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