Tailored PVA/ECM Scaffolds for Cartilage Regeneration

Stocco, Elena; Barbon, Silvia; Dalzoppo, Daniele; Lora, S.; Sartore, Leonardo; Folin, Marcella; Parnigotto, Pier Paolo; Grandi, Claudio

doi:10.1155/2014/762189

Cited by 45 publications

(66 citation statements)

References 43 publications

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“…Although WJ-ECM scaffolds preparation, decellularization and its efficacy on cartilage restoration were previously demonstrated by others [15,16]; in the present study the WJ-derived ECM scaffold is chemically cross-linked, for the first time, with NHS/EDC to enhance the skin regeneration. Based on the results presented, our fabricated scaffold showed no obvious reduction in the ECM components after decellularization and provided an ideal substrate for cellular attachment, penetration and proliferation.…”

Section: Resultsmentioning

confidence: 59%

“…Collagen fibers (the main component of WJ-ECM) can easily undergo cross-linking, by making bridges between the collagen chains [46,47]. Stocoo et al [15] improved mechanical properties of WJ-ECM by physically cross linking it with poly (vinyl alcohol (PVA) hydrogel and producing a composite scaffold. In our study, we did not use any synthetic polymer but EDC/NHS, a non-toxic and biocompatible chemical cross-linker agent recently used for cross-linking porous collagen, collagen-chitosan [48], and collagenphosphorylcholine [49].…”

Section: The Cross-linking Degree Of the Scaffoldmentioning

confidence: 99%

“…Using composite scaffold, combining WJ-derived ECM and synthetic hydrogel polyvinyl alcohol (PVA), Stocco et al [15,16] indicated that this bio-hybrid scaffold had suitable biomechanical properties for cartilage tissue engineering, supporting chondrocyte proliferation. In our study, we investigated the effectiveness of WJ-ECM derived scaffold for reconstruction of damaged skin in a mouse full-thickness wound model.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…Currently, WJ-derived ECM (WJ-ECM) has attracted researcher's attention for its biomedical applications [15,16,21].…”

mentioning

confidence: 99%

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Fabrication of a three dimensional spongy scaffold using human Wharton's jelly derived extra cellular matrix for wound healing

Beiki

Zeynali

Seyedjafari

2017

Materials Science and Engineering: C

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Section: Resultsmentioning

confidence: 59%

Section: The Cross-linking Degree Of the Scaffoldmentioning

confidence: 99%

Section: Accepted Manuscriptmentioning

confidence: 99%

“…Currently, WJ-derived ECM (WJ-ECM) has attracted researcher's attention for its biomedical applications [15,16,21].…”

mentioning

confidence: 99%

See 2 more Smart Citations

Fabrication of a three dimensional spongy scaffold using human Wharton's jelly derived extra cellular matrix for wound healing

Beiki

Zeynali

Seyedjafari

2017

Materials Science and Engineering: C

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“…The physically crosslinked PVA acquires the viscoelastic property comparable with articular cartilage. (Stocco et al, 2014). Mesoporous silica possesses a high surface area and good adsorption capability.…”

Section: Introductionmentioning

confidence: 99%

Surface stiffening and enhanced photoluminescence of ion implanted cellulose – polyvinyl alcohol – silica composite

Shanthini

Sakthivel

Menon

et al. 2016

Carbohydrate Polymers

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Novel Cellulose (Cel) reinforced polyvinyl alcohol (PVA)-Silica (Si) composite which has good stability and in vitro degradation was prepared by lyophilization technique and implanted using N 3+ ions of energy 24 keV in the fluences of 1 × 10 15 , 5 × 10 15 and 1 × 10 16 ions/cm 2 . SEM analysis revealed the formation of microstructures, and improved the surface roughness on ion implantation. In addition to these structural changes, the implantation significantly modified the luminescent, thermal and mechanical properties of the samples. The elastic modulus of the implanted samples has increased by about 50 times compared to the pristine which confirms that the stiffness of the sample surface has increased remarkably on ion implantation. The photoluminescence of the native cellulose has improved greatly due to defect site, dangling bonds and hydrogen passivation. Electric conductivity of the ion implanted samples was improved by about 25%. Hence, low energy ion implantation tunes the mechanical property, surface roughness and further induces the formation of nano structures. MG63 cells seeded onto the scaffolds reveals that with the increase in implantation fluence, the cell attachment, viability and proliferation have improved greatly compared to pristine. The enhancement of cell growth of about 59% was observed in the implanted samples compared to pristine. These properties will enable the scaffolds to be ideal for bone tissue engineering and imaging applications.

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Extracellular Matrix‐Based Biohybrid Materials for Engineering Compliant, Matrix‐Dense Tissues

Bracaglia

Fisher

2015

Adv Healthcare Materials

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An ideal tissue engineering scaffold should not only promote, but take an active role in, constructive remodeling and formation of site appropriate tissue. ECM-derived proteins provide unmatched cellular recognition, and therefore influence cellular response towards predicted remodeling behaviors. Materials built with only these proteins, however, can degrade rapidly or begin too weak to substitute for compliant, matrix-dense tissues. The focus of this review is on biohybrid materials that incorporate polymer components with ECM-derived proteins, to produce a substrate with desired mechanical and degradation properties, as well as actively guide tissue remodeling. Materials are described through four fabrication methods: (1) polymer and ECM-protein fibers woven together, (2) polymer and ECM proteins combined in a bilayer, (3) cell-built ECM on polymer scaffold, and (4) ECM proteins and polymers combined in a single hydrogel. Scaffolds from each fabrication method can achieve characteristics suitable for different types of tissue. In vivo testing has shown progressive remodeling in injury models, and suggests ECM-based biohybrid materials promote a prohealing immune response over single component alternatives. The prohealing immune response is associated with lasting success and long term host maintenance of the implant.

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Tailored PVA/ECM Scaffolds for Cartilage Regeneration

Cited by 45 publications

References 43 publications

Fabrication of a three dimensional spongy scaffold using human Wharton's jelly derived extra cellular matrix for wound healing

Fabrication of a three dimensional spongy scaffold using human Wharton's jelly derived extra cellular matrix for wound healing

Surface stiffening and enhanced photoluminescence of ion implanted cellulose – polyvinyl alcohol – silica composite

Extracellular Matrix‐Based Biohybrid Materials for Engineering Compliant, Matrix‐Dense Tissues

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