Tailored therapeutic release from polycaprolactone-silica hybrids for the treatment of osteomyelitis: antibiotic rifampicin and osteogenic silicates

Gritsch, Lukas; Granel, Henri; Charbonnel, Nicolas; Jallot, Édouard; Wittrant, Yohann; Forestier, Christiane; Lao, Jonathan

doi:10.1039/d1bm02015c

Cited by 7 publications

(4 citation statements)

References 72 publications

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“…This could be attributed to the methyl and methylene groups in RFP. These results substantiate the loading of RFP in PCL−RFP NPs as previously verified by spectrophotometry [22] . The peaks are similar to reported studies on PCL [23] …”

Section: Resultssupporting

confidence: 91%

Lipase‐Responsive Rifampicin‐Based Biodegradable PCL Nanocarrier for Antibacterial Treatment

et al. 2023

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Rifampicin (RFP) is a first‐line drug used to treat a variety of infections, including wound infections but has limitations in its use due to its toxicity. Hence, an urgent need exists for the development of suitable carriers for the delivery of the antibiotic. In this study, a novel approach is introduced for drug administration, employing stimulus‐responsive carriers to achieve an on‐demand strategy. This innovative method aims to minimize the dosage and frequency of drug administration, consequently lowering cytotoxicity levels. We used the lipases‐sensitive polycaprolactone (PCL) to produce nanocomposites loaded with rifampicin (PCL−RFP NPs). Nanoparticles were prepared by a single‐step emulsion solvent evaporation method. The size distribution of blank nanoparticles (PCL NPs) and PCL−RFP NPs were 172±30 nm and 229±58 nm, respectively. The liberation of RFP from PCL−RFP NPs was monitored over a period of 72 h in the absence and the presence of lipase was 9.46±0.24 % and 53.3±3.33 %, respectively, indicating responsive behavior. The minimum inhibitory concentration to lipase‐expressing Staphylococcus aureus (S. aureus) of PCL−RFP NPs was significantly improved compared to the free drug. Cytotoxicity tests using human dermal fibroblasts showed that the nanocomposites had better biocompatible when compared to the free drug. These findings indicate that the developed nanocomposite carriers have the potential to be promising candidates for delivering antibiotics in the field of biomedicine.

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Section: Resultssupporting

confidence: 91%

Lipase‐Responsive Rifampicin‐Based Biodegradable PCL Nanocarrier for Antibacterial Treatment

et al. 2023

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“…Consistently, it is worth noting that the sol–gel route, we used to synthesize silicate hybrids, is conducted under mild conditions and at room temperature, opening up new possibilities for doping with such compounds. Very recently, we succeeded in doping poly(caprolactone)–silica hybrids with rifampicin for tailored therapeutic release of antibiotics in the treatment of osteomyelitis . Thus, our sol–gel process allows the introduction of fisetin in situ during the synthesis of BG–PCL hybrids.…”

Section: Introductionmentioning

confidence: 98%

“…Very recently, we succeeded in doping poly(caprolactone)–silica hybrids with rifampicin for tailored therapeutic release of antibiotics in the treatment of osteomyelitis. 16 Thus, our sol–gel process allows the introduction of fisetin in situ during the synthesis of BG–PCL hybrids. This doping-in-the-bulk technique contrasts with the usual adsorption techniques employed for organic loading, which commonly results in a nonsustainable release with an immediate burst of the loaded agent and limited long-term potential.…”

Section: Introductionmentioning

confidence: 99%

Osteogenic Effect of Fisetin Doping in Bioactive Glass/Poly(caprolactone) Hybrid Scaffolds

et al. 2022

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Treating large bone defects or fragile patients may require enhancing the bone regeneration rate to overcome a weak contribution from the body. This work investigates the osteogenic potential of nutrient fisetin, a flavonoid found in fruits and vegetables, as a doping agent inside the structure of a SiO 2 –CaO bioactive glass–poly(caprolactone) (BG–PCL) hybrid scaffold. Embedded in the full mass of the BG–PCL hybrid during one-pot synthesis, we demonstrate fisetin to be delivered sustainably; the release follows a first-order kinetics with active fisetin concentration being delivered for more than 1 month (36 days). The biological effect of BG–PCL–fisetin-doped scaffolds (BG–PCL–Fis) has been highlighted by in vitro and in vivo studies. A positive impact is demonstrated on the adhesion and the differentiation of rat primary osteoblasts, without an adverse cytotoxic effect. Implantation in critical-size mouse calvaria defects shows bone remodeling characteristics and remarkable enhancement of bone regeneration for fisetin-doped scaffolds, with the regenerated bone volume being twofold that of nondoped scaffolds and fourfold that of a commercial trabecular bovine bone substitute. Such highly bioactive materials could stand as competitive alternative strategies involving biomaterials loaded with growth factors, the use of the latter being the subject of growing concerns.

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“…It is imperative to develop innovative treatment methods. At present, a large number of antibiotic sustained-release materials have been developed to treat osteomyelitis ( Hasan et al, 2019 ; Xu et al, 2021 ; Feng et al, 2022 ; Gritsch et al, 2022 ; Wu et al, 2022 ), but the treatment effect is still not ideal and the recurrence rate is high. Zhou ( Zhang et al, 2023 ) et al reported Vancomycin-loaded silk fibroin microspheres in an injectable hydrogel for chronic osteomyelitis therapy, demonstrating a sustained-release profile and good biocompatibility, making it promising for application in osteomyelitis treatment.…”

Section: Introductionmentioning

confidence: 99%

Preparation of fish collagen and vancomycin microspheres based on microfluidic technology and its application in osteomyelitis

Hu,

Tang,

et al. 2023

Front. Bioeng. Biotechnol.

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At present, the clinical treatment of osteomyelitis and osteomyelitis-induced bone defects is challenging, easy to recur, drug toxic side effects, secondary or multiple surgeries, etc. The design of biodegradable composite biomaterials to improve antibiotics in the local precise anti-infection at the same time to complete the repair of bone defects is the current research hot spot. Herein, a composite hydrogel with a double bond at the end (FA-MA) was prepared by affinity addition reaction between fish collagen (FA) and methacrylic anhydride (MA) under photoinitiator initiation conditions, then, FA-MA was amino-activated by EDC/NHC, and vancomycin was attached to FA-MA via amide bonding to prepare FA-MA-Van hydrogels, and finally, the composite hydrogel microspheres were prepared by microfluidic technology. The structure of the hydrogel was confirmed by SEM (elemental analysis), optical microscopy, FTIR, and XPS to confirm the successful preparation. The composite hydrogel microspheres showed the better antimicrobial effect of hydrogel microspheres by bacterial coated plate experiments and SEM morphology results, with the antimicrobial class reaching 99.8%. The results of immunofluorescence staining and X-ray experiments showed that the hydrogel microspheres had a better effect on promoting bone repair. This engineered design of hydrogel microspheres provides clinical significance for treating osteomyelitis at a later stage.

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Tailored therapeutic release from polycaprolactone-silica hybrids for the treatment of osteomyelitis: antibiotic rifampicin and osteogenic silicates

Abstract: Polycaprolactone/silica hybrids offer a dual therapeutic effect: the promotion of bone regeneration thanks to silicate ions and strong antibacterial properties against the risk of osteomyelitis.

Cited by 7 publications

References 72 publications

Lipase‐Responsive Rifampicin‐Based Biodegradable PCL Nanocarrier for Antibacterial Treatment

Lipase‐Responsive Rifampicin‐Based Biodegradable PCL Nanocarrier for Antibacterial Treatment

Osteogenic Effect of Fisetin Doping in Bioactive Glass/Poly(caprolactone) Hybrid Scaffolds

Preparation of fish collagen and vancomycin microspheres based on microfluidic technology and its application in osteomyelitis

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