Tailored UV-laser source for fluorescence spectroscopy of biomolecules

Tiihonen, Mikael; Pasiskevicius, Valdas; Laurell, Fredrik

doi:10.1016/j.optlaseng.2005.03.016

Cited by 7 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The nonlinear SFM process between the pump and the signal creates UV radiation at 293 nm, which is coupled out of the cavity by the dichroic mirror. Further details of this laser can be found elsewhere [16,18]. To facilitate simple switching between the excitation wavelength (without changing the PPKTP crystal and realign the laser), we used a commercially available nitrogen gas laser (Oriel 79111) for excitation at 337 nm.…”

Section: Uv Lasersmentioning

confidence: 99%

Detection of fluorescence spectra of individual bioaerosol particles

et al. 2005

View full text Add to dashboard Cite

We present initial results of a measurement system designed for detecting the fluorescence spectrum of individual particles of biological warfare agent (BWA). A compact optical parametric oscillator with intracavity sum-frequency mixing and a commercial Nitrogen gas laser was used as excitation sources to generate 293 nm or 337 nm UV laser irradiation. The pulsed lasers and a photomultiplier tube (PMT) array based spectrometer were triggered by a red laserdiode and a PMT detector that sensed the presence of a particle typical of size 5-20 µm in diameter. The spectral detection part of the system consisted of a grating and a PMT array with 32 channels, which measured fluorescence in the wavelength from 280 nm to 800 nm. The detector system was used to demonstrate the measurement of laser induced fluorescence spectra of individual BWA simulant particles by excitation of single UV laser pulses. The spectrum obtained by averaging spectra from several BWA aerosol simulant particles were found generally similar, but not identical, to the fluorescence spectrum obtained from water solutions containing the same particles dissolved.

show abstract

Section: Uv Lasersmentioning

confidence: 99%

Detection of fluorescence spectra of individual bioaerosol particles

et al. 2005

View full text Add to dashboard Cite

show abstract

“…The usefulness of sum-frequency generation to produce tunable UV radiation from nanosecond solid-state laser sources was demonstrated by several authors for alexandrite lasers [5][6][7], Ti:sapphire lasers [8][9][10][11][12][13][14], Cr-doped colquiriite lasers [15], or OPOs [16][17][18][19][20] in the past. However, none of the reported configurations would be able to fulfill the requirements of a laser for a compact, versatile ion mobility spectrometer.…”

Section: Introductionmentioning

confidence: 99%

Compact, passively Q-switched, all-solid-state master oscillator-power amplifier-optical parametric oscillator (MOPA-OPO) system pumped by a fiber-coupled diode laser generating high-brightness, tunable, ultraviolet radiation

Peuser¹,

Platz²,

Fix³

et al. 2009

Appl. Opt.

View full text Add to dashboard Cite

We report on a compact, tunable ultraviolet laser system that consists of an optical parametric oscillator (OPO) and a longitudinally diode-pumped Nd:YAG master oscillator-power amplifier (MOPA). The pump energy for the whole laser system is supplied via a single delivery fiber. Nanosecond pulses are produced by an oscillator that is passively Q-switched by a Cr(4+):YAG crystal. The OPO is pumped by the second harmonic of the Nd:YAG MOPA. Continuously tunable radiation is generated by an intracavity sum-frequency mixing process within the OPO in the range of 245-260 nm with high beam quality. Maximum pulse energies of 1.2 mJ were achieved, which correspond to an optical efficiency of 3.75%, relating to the pulse energy of the MOPA at 1064 nm.

show abstract

“…As most bio-molecules show strong absorption in the ultraviolet (UV) spectral region ranging from 280 to 340 nm [1], an efficient UV lighting source such as light emitting diodes (LEDs) is expected to be a crucial component for nextgeneration biological detection, biological imaging, disease analysis, optical catalysis, water purification applications, etc. Consequently there is an increasing emphasis on development of ultraviolet light emitting diodes (UV-LEDs) based on III-nitride semiconductors.…”

Section: Introductionmentioning

confidence: 99%

The 310–340 nm ultraviolet light emitting diodes grown using a thin GaN interlayer on a high temperature AlN buffer

Wang¹,

Lee²,

Bai³

et al. 2008

J. Phys. D: Appl. Phys.

View full text Add to dashboard Cite

Previously, we reported that a thin GaN interlayer approach has been developed for growth of 340 nm ultraviolet light emitting diodes (UV-LEDs) with significantly improved performance. In this paper, more recent results on the further development of UV-LEDs with shorter wavelengths are reported, and the limitation of the wavelength of the UV-LEDs that can be pushed to, while retaining high device performance using the approach has been investigated. Transmission electron microscopy and device-performance data, including electrical and optical characteristics, indicated that the thin GaN interlayer approach can be effectively employed for growth of UV-LEDs to an emission wavelength approaching at least 300 nm. The approach should be taken into account in growth of UV-LEDs on sapphire substrates, as it provides a simple but effective growth method to achieve UV-LEDs with high performance. This paper also reports that a micro-LED array using the UV-LED wafer has been successfully fabricated, offering versatile micro-structured UV light sources for a wide range of applications.

show abstract

Tailored UV-laser source for fluorescence spectroscopy of biomolecules

Cited by 7 publications

References 21 publications

Detection of fluorescence spectra of individual bioaerosol particles

Detection of fluorescence spectra of individual bioaerosol particles

Compact, passively Q-switched, all-solid-state master oscillator-power amplifier-optical parametric oscillator (MOPA-OPO) system pumped by a fiber-coupled diode laser generating high-brightness, tunable, ultraviolet radiation

The 310–340 nm ultraviolet light emitting diodes grown using a thin GaN interlayer on a high temperature AlN buffer

Contact Info

Product

Resources

About