Tailoring immobilization of immunoglobulin by excimer laser for biosensor applications

Abstract

The sheltered transfer and immobilization of rabbit anti-human antiserum immunoglobulin G (IgG) by matrix-assisted pulsed laser evaporation (MAPLE) are reported. The iced targets submitted to laser irradiation consisted of 0.2-2 mg/mL IgG blended or not with lipid (L-α-phosphatidylcholine dipalmitoyl) dissolved in distilled water-based saline buffer. Thin IgG coatings were obtained at room temperature onto glass, fused silica, or silicon substrates. Ten thousand subsequent laser pulses of 0.33, 0.5, or 0.67 J/cm(2) fluence were applied for the synthesis of each sample. Morphology and composition of the thin films were studied by optical, scanning, and atomic force microscopy and Fourier transformed infrared spectrometry. Optical labeling methods such as spectrofluorimetry and fluorescence microscopy were selected to verify the biosensor transduction principle because of their high sensitivity for detecting low amounts of antigen (IgG). Protein immobilization to the substrate surface was demonstrated for all obtained structures after immersion in the donkey anti-rabbit secondary antibody solution. The IgG transfer and immobilization onto substrates were improved by addition of lipid to MAPLE solutions.

Molecular organization in MAPLE-deposited conjugated polymer thin films and the implications for carrier transport characteristics

“…Together with its advantages of being a vacuum‐based deposition technique, the MAPLE technique enables the production of films with unique morphologies and associated physical properties . With its unprecedented capabilities, MAPLE has readily been exploited for growing thin polymer films for a wide range of applications including sensors, drug delivery and medical implants . Recently, promising findings have also been reported on the properties of MAPLE deposited conjugated polymer films for solar cells, organic light emitting diodes, and other organic electronic applications .…”

Combinatorial matrix-assisted pulsed laser evaporation: Single-step synthesis of biopolymer compositional gradient thin film assemblies

“…14 A recent comprehensive review on MAPLE deposition of organic, biological, and nanoparticle thin films illustrated large potential applications of thin coatings obtained by this method. 15 Recently, MAPLE was applied to obtain uniform, ultrastable, and nanostructured glassy polymer films with superior thermal and kinetic stability.…”

Biopolymer Thin Films Synthesized by Advanced Pulsed Laser Techniques

“…Applications targeted development of biosensing, chemical sensing, and biochemical analysis, as well as drug delivery systems and the developing of a new generation of implants [19,[82][83][84][85][86]. In MAPLE, the laser-induced material ejection is generated backward from a solid cryogenic target.…”