Tailoring of the rheological properties of bioinks to improve bioprinting and bioassembly for tissue replacement

Chopin-Doroteo, Mario; Mandujano-Tinoco, Edna Ayerim; Krötzsch, Edgar

doi:10.1016/j.bbagen.2020.129782

Cited by 52 publications

(46 citation statements)

References 56 publications

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“…The mechanical properties of the bioink play an important role in maintaining the desired tissue shape after bioprinting and influence the behavior of cells seeded inside the bioprinted construct [3,[7][8][9]. Moreover, achieving the desired print resolution for the bioprinted construct depends on the rheological properties of bioinks [10][11][12][13]. Therefore, bioinks should possess appropriate rheological, mechanical, biocompatible, and biofunctional properties for the target tissue [6,14].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the porous structure of chitosan allowed cells and nutrients to diffuse and migrate through the structure and can be formed by crosslinking chitosan with a variety of agents [8,18,62]. However, to successfully engineer mature bioprinted tissues for drug testing, a bioink should be printed as a desired structure to maintain its integrity over the culture period to support cell growth and differentiation [13,40,42]. The bioink must also support cell-material interactions to promote cell attachment and migration and possibly deliver differentiation cues [3,6,60,65].…”

Section: Introductionmentioning

confidence: 99%

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Physical and Mechanical Characterization of Fibrin-Based Bioprinted Constructs Containing Drug-Releasing Microspheres for Neural Tissue Engineering Applications

et al. 2021

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Three-dimensional bioprinting can fabricate precisely controlled 3D tissue constructs. This process uses bioinks—specially tailored materials that support the survival of incorporated cells—to produce tissue constructs. The properties of bioinks, such as stiffness and porosity, should mimic those found in desired tissues to support specialized cell types. Previous studies by our group validated soft substrates for neuronal cultures using neural cells derived from human-induced pluripotent stem cells (hiPSCs). It is important to confirm that these bioprinted tissues possess mechanical properties similar to native neural tissues. Here, we assessed the physical and mechanical properties of bioprinted constructs generated from our novel microsphere containing bioink. We measured the elastic moduli of bioprinted constructs with and without microspheres using a modified Hertz model. The storage and loss modulus, viscosity, and shear rates were also measured. Physical properties such as microstructure, porosity, swelling, and biodegradability were also analyzed. Our results showed that the elastic modulus of constructs with microspheres was 1032 ± 59.7 Pascal (Pa), and without microspheres was 728 ± 47.6 Pa. Mechanical strength and printability were significantly enhanced with the addition of microspheres. Thus, incorporating microspheres provides mechanical reinforcement, which indicates their suitability for future applications in neural tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Physical and Mechanical Characterization of Fibrin-Based Bioprinted Constructs Containing Drug-Releasing Microspheres for Neural Tissue Engineering Applications

et al. 2021

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“…Some biomaterials such as alginate have innate shear thinning properties. The addition of polymers, such as poloxamer 407, gellan gum, and gelMA to bioinks have also been shown to increase the shear-thinning abilities of the bioink [54]. Overall, the ideal rheological behaviour of a bioink designed for extrusion-based bioprinting should: (1) display gel behaviour given by the dominance of elasticity over viscous behaviour prior to dispensing, (2) show predominantly viscous behaviour over elastic behaviour during flow through the printing nozzle, and (3) return as closely as possible to the original gel state immediately after deposition [55].…”

Section: Shear-thinningmentioning

confidence: 99%

Translational Application of 3D Bioprinting for Cartilage Tissue Engineering

et al. 2021

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Cartilage is an avascular tissue with extremely limited self-regeneration capabilities. At present, there are no existing treatments that effectively stop the deterioration of cartilage or reverse its effects; current treatments merely relieve its symptoms and surgical intervention is required when the condition aggravates. Thus, cartilage damage remains an ongoing challenge in orthopaedics with an urgent need for improved treatment options. In recent years, major advances have been made in the development of three-dimensional (3D) bioprinted constructs for cartilage repair applications. 3D bioprinting is an evolutionary additive manufacturing technique that enables the precisely controlled deposition of a combination of biomaterials, cells, and bioactive molecules, collectively known as bioink, layer-by-layer to produce constructs that simulate the structure and function of native cartilage tissue. This review provides an insight into the current developments in 3D bioprinting for cartilage tissue engineering. The bioink and construct properties required for successful application in cartilage repair applications are highlighted. Furthermore, the potential for translation of 3D bioprinted constructs to the clinic is discussed. Overall, 3D bioprinting demonstrates great potential as a novel technique for the fabrication of tissue engineered constructs for cartilage regeneration, with distinct advantages over conventional techniques.

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“…While ideal for generating 3D scaffolds of high printing resolution, bioinks with high viscosity are not suitable for the extrusion of living cells, given the high shear stress, certainly caused during extrusion of highly viscous bioinks which could damage the cell membranes, with obvious negative impact on cell viability [134]. Generally, a bioink is considered bioprintable when the viscosity of the fluid decreases under shear (shear thinning).…”

Section: Extrusion Bioprintingmentioning

confidence: 99%

Modelling Human Physiology on-Chip: Historical Perspectives and Future Directions

2021

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For centuries, animal experiments have contributed much to our understanding of mechanisms of human disease, but their value in predicting the effectiveness of drug treatments in the clinic has remained controversial. Animal models, including genetically modified ones and experimentally induced pathologies, often do not accurately reflect disease in humans, and therefore do not predict with sufficient certainty what will happen in humans. Organ-on-chip (OOC) technology and bioengineered tissues have emerged as promising alternatives to traditional animal testing for a wide range of applications in biological defence, drug discovery and development, and precision medicine, offering a potential alternative. Recent technological breakthroughs in stem cell and organoid biology, OOC technology, and 3D bioprinting have all contributed to a tremendous progress in our ability to design, assemble and manufacture living organ biomimetic systems that more accurately reflect the structural and functional characteristics of human tissue in vitro, and enable improved predictions of human responses to drugs and environmental stimuli. Here, we provide a historical perspective on the evolution of the field of bioengineering, focusing on the most salient milestones that enabled control of internal and external cell microenvironment. We introduce the concepts of OOCs and Microphysiological systems (MPSs), review various chip designs and microfabrication methods used to construct OOCs, focusing on blood-brain barrier as an example, and discuss existing challenges and limitations. Finally, we provide an overview on emerging strategies for 3D bioprinting of MPSs and comment on the potential role of these devices in precision medicine.

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Tailoring of the rheological properties of bioinks to improve bioprinting and bioassembly for tissue replacement

Cited by 52 publications

References 56 publications

Physical and Mechanical Characterization of Fibrin-Based Bioprinted Constructs Containing Drug-Releasing Microspheres for Neural Tissue Engineering Applications

Physical and Mechanical Characterization of Fibrin-Based Bioprinted Constructs Containing Drug-Releasing Microspheres for Neural Tissue Engineering Applications

Translational Application of 3D Bioprinting for Cartilage Tissue Engineering

Modelling Human Physiology on-Chip: Historical Perspectives and Future Directions

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