Tailoring RGD local surface density at the nanoscale toward adult stem cell chondrogenic commitment

Lagunas, Anna; Tsintzou, Iro; Vida, Yolanda; Collado, Daniel; Pérez‐Inestrosa, Ezequiel; Pereira, Cristina Rodríguez; Magalhães, Joana; Andrades, José A.; Samitier, J.

doi:10.1007/s12274-016-1382-5

Cited by 19 publications

(73 citation statements)

References 51 publications

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“…That is, each dendrimer provided a single site for integrin binding, thereby allowing a direct correlation from dendrimer distribution (as seen in the AFM images) and the RGD distribution available for cell adhesion. Moreover, no significant variations were found in the CA values obtained for the different nanopattern configurations that may influence cell adhesion 29 . These characteristics make dendrimer nanopatterns on biocompatible surfaces suitable substrates through which modulate and study cell behavior.…”

Section: Representative Resultsmentioning

confidence: 88%

Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation

Casanellas

Lagunas

Tsintzou

et al. 2018

JoVE

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Cellular adhesion and differentiation is conditioned by the nanoscale disposition of the extracellular matrix (ECM) components, with local concentrations having a major effect. Here we present a method to obtain large-scale uneven nanopatterns of arginine-glycine-aspartic acid (RGD)-functionalized dendrimers that permit the nanoscale control of local RGD surface density. Nanopatterns are formed by surface adsorption of dendrimers from solutions at different initial concentrations and are characterized by water contact angle (CA), X-ray photoelectron spectroscopy (XPS), and scanning probe microscopy techniques such as scanning tunneling microscopy (STM) and atomic force microscopy (AFM). The local surface density of RGD is measured using AFM images by means of probability contour maps of minimum interparticle distances and then correlated with cell adhesion response and differentiation. The nanopatterning method presented here is a simple procedure that can be scaled up in a straightforward manner to large surface areas. It is thus fully compatible with cell culture protocols and can be applied to other ligands that exert concentration-dependent effects on cells.

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Section: Representative Resultsmentioning

confidence: 88%

Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation

Casanellas

Lagunas

Tsintzou

et al. 2018

JoVE

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“…The transforming growth factor (TGF) superfamily of proteins induces precartilage condensation by the regulation of the cell matrix glycoprotein fibronectin (FN), with the cells preferably accumulating in the regions of stronger cell-matrix adhesive interactions [ 5 , 20 ]. Here we used dendrimer-based nanopatterns of RGD to control the local surface adhesiveness of poly(L-lactic acid) (PLLA) substrates during chondrogenic differentiation [ 17 ]. Dendrimers adsorbed onto non-charged surfaces in a liquid-like order with a defined spacing [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

“…A large nanospacing (161 nm) between the RGD motifs led to a reduced spreading area and a higher chondrogenic induction [ 14 ]. In previous works, we used RGD dendrimer-based nanopatterns to evaluate the effects of local RGD surface density on cell adhesion and differentiation [ 15 , 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

The Janus Role of Adhesion in Chondrogenesis

Casanellas

Lagunas

Vida

et al. 2020

IJMS

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Tackling the first stages of the chondrogenic commitment is essential to drive chondrogenic differentiation to healthy hyaline cartilage and minimize hypertrophy. During chondrogenesis, the extracellular matrix continuously evolves, adapting to the tissue adhesive requirements at each stage. Here, we take advantage of previously developed nanopatterns, in which local surface adhesiveness can be precisely tuned, to investigate its effects on prechondrogenic condensation. Fluorescence live cell imaging, immunostaining, confocal microscopy and PCR analysis are used to follow the condensation process on the nanopatterns. Cell tracking parameters, condensate morphology, cell–cell interactions, mechanotransduction and chondrogenic commitment are evaluated in response to local surface adhesiveness. Results show that only condensates on the nanopatterns of high local surface adhesiveness are stable in culture and able to enter the chondrogenic pathway, thus highlighting the importance of controlling cell–substrate adhesion in the tissue engineering strategies for cartilage repair.

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“…Previous studies evaluating the influence of the RGD domain in cell adhesion and proliferation showed that the use of dendrimers as scaffolds to distribute the RGD domain through a surface promotes a higher interaction than a homogenous linear layer of RGD peptides [16]. Moreover, nanopatterns of RGD-decorated dendrimers showed that cell adhesion, proliferation and differentiation are affected by local RGD surface density [17,18]. Therefore, the way in which the RGD domain is exposed influences cell response.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, studies of RGD-decorated dendrimer nanopatterning showed that cell adhesion and proliferation are affected by local RGD surface density, differing from the observed results when using a homogenous linear layer of RGD peptides. The local RGD surface density acts as a regulator of chondrogenic commitment, and intermediate adhesiveness of cells to the substrates favors mesenchymal cell condensation and early chondrogenic differentiation [17,18].…”

Section: Introductionmentioning

confidence: 99%

Dendritic Scaffold onto Titanium Implants. A Versatile Strategy Increasing Biocompatibility

et al. 2020

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Osseointegration of metal prosthetic implants is a yet unresolved clinical need that depends on the interplay between the implant surface and bone cells. The lack of a relationship between bone cells and metal has traditionally been solved by coating the former with “organic” ceramics, such as hydroxyapatite. A novel approach is hereby presented, immobilizing covalently dendrimeric structures onto titanium implants. Amide-based amino terminal dendrons were synthetized and coupled to titanium surfaces in a versatile and controlled way. The dendritic moieties provide an excellent scaffold for the covalent immobilization of bioactive molecules, such as extracellular matrix (ECM) protein components or antibiotics. Herein, tripeptide arginine-glycine-aspartic acid (RGD) motifs were used to decorate the dendritic scaffolds and their influence on cell adhesion and proliferation processes was evaluated.

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Tailoring RGD local surface density at the nanoscale toward adult stem cell chondrogenic commitment

Cited by 19 publications

References 51 publications

Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation

Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation

The Janus Role of Adhesion in Chondrogenesis

Dendritic Scaffold onto Titanium Implants. A Versatile Strategy Increasing Biocompatibility

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