Tailoring the assembly of collagen fibers in alginate microspheres

Lehnert, Sarah; Sikorski, Pawel

doi:10.1016/j.msec.2020.111840

Cited by 10 publications

(5 citation statements)

References 51 publications

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“…The collagen fiber assembly within the microspheres was influenced by the alginate gelling process. [ 71 ] The collagen in the microspheres assembled into much shorter fiber bundles (Figure 3B, yellow arrows). According to Lutz et al, a reduced collagen crosslinking results in collagen fibers with a curly morphology and with the fibers being less aligned to each other.…”

Section: Resultsmentioning

confidence: 99%

Application of Temporary, Cell‐Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels

Lehnert

Sikorski

2021

Macromolecular Bioscience

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Mimicking the complexity of natural tissue is a major challenge in the field of tissue engineering. Here, a facile 2‐step fabrication method to prepare 3D constructs with distinct regions of high cell concentrations and without the need for elaborate equipment is proposed. The initial incorporation of cells in a sacrificial alginate matrix allows the addition of other, cell relevant biopolymers, such as, collagen to form a spatially confined, interpenetrating network at the microscale. A layered structure at the macroscale can be achieved by incorporating these cell‐containing microspheres in thin collagen layers. Cells are locally released by de‐gelling the alginate matrix and their attachment to the collagen hydrogel layers has been studied. The use of the murine pre‐osteoblast cell line MC3T3‐E1 as an example cell line shows that the cells behave differently in their cell migration pattern based on the initial composition of the alginate microspheres.

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Section: Resultsmentioning

confidence: 99%

Application of Temporary, Cell‐Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels

Lehnert

Sikorski

2021

Macromolecular Bioscience

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“…The addition of natural or synthetic ECM components or native cell adhesion ligands have been reported for alginate microspheres ( Lehnert and Sikorski, 2021 ). These methods provide more physiologically relevant models for studying gradient-dependent cellular responses in the context of organoids research.…”

Section: Discussionmentioning

confidence: 99%

Deep learning-assisted concentration gradient generation for the study of 3D cell cultures in hydrogel beads of varying stiffness

Anagnostidis,

Tiwari,

Gielen

2024

Front. Bioeng. Biotechnol.

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The study of dose-response relationships underpins analytical biosciences. Droplet microfluidics platforms can automate the generation of microreactors encapsulating varying concentrations of an assay component, providing datasets across a large chemical space in a single experiment. A classical method consists in varying the flow rate of multiple solutions co-flowing into a single microchannel (producing different volume fractions) before encapsulating the contents into water-in-oil droplets. This process can be automated through controlling the pumping elements but lacks the ability to adapt to unpredictable experimental scenarios, often requiring constant human supervision. In this paper, we introduce an image-based, closed-loop control system for assessing and adjusting volume fractions, thereby generating unsupervised, uniform concentration gradients. We trained a shallow convolutional neural network to assess the position of the laminar flow interface between two co-flowing fluids and used this model to adjust flow rates in real-time. We apply the method to generate alginate microbeads in which HEK293FT cells could grow in three dimensions. The stiffnesses ranged from 50 Pa to close to 1 kPa in Young modulus and were encoded with a fluorescent marker. We trained deep learning models based on the YOLOv4 object detector to efficiently detect both microbeads and multicellular spheroids from high-content screening images. This allowed us to map relationships between hydrogel stiffness and multicellular spheroid growth.

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“…[32] Copyright 2021, The Royal Society of Chemistry. Internal stimuli pH-responsive PEG, TEG Inward diffusion [47,100,118,123,126,129] Redox-responsive PEG Inward diffusion [56,136,156] Enzyme-responsive ALG Cross-linking-codependent [14,27,106,124,130,132,158] Electroresponsive PLGA, PEG Inward diffusion [70,146,196] ROS-responsive PEG, PLGA Self-assembled [149,150,160,162,165] External stimuli Thermoresponsive PEG, PLGA In situ formation [7,30,34,42,67,170,171,173,175,17 8,181] Light-responsive PLGA, ALG Self-assembled [9,28,113,183,185] Magnetic-responsive ALG, HA Cross-linking-codependent [29,166,193,194,195] Mechanoresponsive PEG, PCL Inward diffusion [146,148,197,199,200] Ultrasound-responsive PEG Inward diffusion [14,163,201,…”

Section: Ph-responsive Nanocomposite Hydrogelsmentioning

confidence: 99%

“…The most commonly known type of nanocomposite hydrogel consists of a polymeric matrix that contains nanomaterials and can be assembled using noncovalent interactions or covalent interactions. [104][105][106] If an independent nanocomposite hydrogel can be formed by successful assembly or nanoparticle interaction between two building blocks, then the remaining components serve as filler agents for the network such as PEG and triethylene glycol (TEG). Meanwhile, codependent nanocomposite hydrogels contain hybrid systems whose assembly is dependent on simultaneous particle-hydrogel interactions.…”

Section: The Assembly Process Of Responsive Nanocomposite Hydrogels I...mentioning

confidence: 99%

Responsive Hydrogels Based on Triggered Click Reactions for Liver Cancer

Zhu

et al. 2022

Advanced Materials

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Tailoring the assembly of collagen fibers in alginate microspheres

Cited by 10 publications

References 51 publications

Application of Temporary, Cell‐Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels

Application of Temporary, Cell‐Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels

Deep learning-assisted concentration gradient generation for the study of 3D cell cultures in hydrogel beads of varying stiffness

Responsive Hydrogels Based on Triggered Click Reactions for Liver Cancer

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