Tailoring the porosity and pore size of electrospun synthetic human elastin scaffolds for dermal tissue engineering

Rnjak‐Kovacina, Jelena; Wise, Steven G.; Li, Zhe; Maitz, Peter; Young, Cara J.; Wang, Yiwei; Weiss, Anthony S.

doi:10.1016/j.biomaterials.2011.05.065

Cited by 280 publications

(190 citation statements)

References 46 publications

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“…18,19) Figure 3b shows that porosity was reduced as fiber diameter decreased. Similar to the findings in this study, Kaur et al 20) and Varesano et al 21) reported that porosity was reduced as fiber size decreased.…”

Section: )mentioning

confidence: 99%

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Intermolecular Interactions and the Release Pattern of Electrospun Curcumin-Polyvinyl(pyrrolidone) Fiber

Rahma

Munir

Khairurrijal

et al. 2016

Biological & Pharmaceutical Bulletin

167

104

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An electrospun fiber of polyvinyl(pyrrolidone) (PVP)-Tween 20 (T20) with curcumin as the encapsulated drug has been developed. A study of intermolecular interactions was performed using Raman spectroscopy, Fourier transform infrared (FT-IR), differential scanning calorimetry (DSC), and X-ray diffraction (XRD).The Raman and FT-IR studies showed that curcumin preferrably interacted with T20 and altered PVP chain packing, as supported by XRD and physical stability data. The hydroxyl stretching band in PVP shifted to a lower wavenumber with higher intenstity in the presence of curcumin and PVP, indicating that hydrogen bond formation is more intense in a curcumin or curcumin-T20 containing fiber. The thermal pattern of the fiber did not indicate phase separation. The conversion of curcumin into an amorphous state was confirmed by XRD analysis. An in vitro release study in phosphate buffer pH 6.8 showed that intermolecular interactions between each material influenced the drug release rate. However, low porosity was found to limit the hydrogen bond-mediated release.Key words curcumin; fiber; electrospinning; interaction; porosity; polymeric drug delivery system Curcumin is the major constituent of turmeric rhizome (Curcuma sp.).1) Curcumin has a number of demonstrated pharmacological effects, such as antioxidant, anti-inflammatory, anticancer, hepatoprotective, 1) antimicrobial, and antiviral. 2)Clinical trials have proven that curcumin is well tolerated by the body, with a maximum dose of 12 g daily.3) Unfortunately, the efficacy of curcumin is limited by its poor solubility in water, as well as its instability under light, heat, and alkaline conditions.1) The solubility of curcumin in aqueous buffer (pH 5) is reported to be 11 ng/mL.3) Moreover, the absorption of curcumin in the gastrointestinal tract is very low, leading to poor bioavailability (in animals and humans).2) Therefore, designing an effective delivery system is necessary in order to address the limitations of curcumin use. 1-3)A wide range of drug delivery systems has been developed to improve the bioavailability of poorly soluble drugs, including fast-dissolving tablets, 4) incorporation into a hydrophilic complex, 1) micellization, and solid dispersion. 5) Electrospinning, a technique of producing thin strands of fiber using high voltage, has opened up opportunities in the development of drug delivery systems. An optimized electrospinning process can produce nano-sized fibers. 6)The high surface area and porosity of these electrospun fibers are advantageous in their use as carriers for poorly water-soluble drugs.5) The high surface area generally increases the dissolution rate of the incorporated drug, thus potentially enhancing its bioavailability. 5)Therefore, the incorporation of curcumin into an electrospun fiber is expected to improve its oral bioavailability.Tailoring a suitable drug-loaded fiber requires careful selection of materials, in addition to an optimized production process and environment. Generally, the drug is mixed with a polymer s...

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Section: )mentioning

confidence: 99%

“…High porosity indicates that the number of intermolecular bondings formed was low. 19) Higher PVP concentration in the precursor solution of PCT10 has more available binding sites for other materials, 22) resulting in fewer intermolecular interactions. Therefore, porosity increased in fibers with higher PVP concentrations.…”

Section: )mentioning

confidence: 99%

Intermolecular Interactions and the Release Pattern of Electrospun Curcumin-Polyvinyl(pyrrolidone) Fiber

Rahma

Munir

Khairurrijal

et al. 2016

Biological & Pharmaceutical Bulletin

167

104

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“…14 Current strategies to introduce porosity into three-dimensional scaffolds are usually performed under nonphysiological conditions. 32,33,68,69 Therefore, the biomedical applications of these systems only allow for cell seeding after the fabrication process, and as a result, nonuniform cell distribution can rise up as a problem. As a proof of concept, we also tested the capacity of nanofibrous self-assembled PA/PEG composite matrices as three-dimensional (3D) scaffolds that allow for a cell-friendly fabrication process and in situ application of engineered scaffolds.…”

Section: Biomacromoleculesmentioning

confidence: 99%

Self-Assembled Peptide Amphiphile Nanofibers and PEG Composite Hydrogels as Tunable ECM Mimetic Microenvironment

Göktas

Çınar

Orujalipoor³

et al. 2015

Biomacromolecules

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Natural extracellular matrix (ECM) consists of complex signals interacting with each other to organize cellular behavior and responses. This sophisticated microenvironment can be mimicked by advanced materials presenting essential biochemical and physical properties in a synergistic manner. In this work, we developed a facile fabrication method for a novel nanofibrous self-assembled peptide amphiphile (PA) and poly(ethylene glycol) (PEG) composite hydrogel system with independently tunable biochemical, mechanical, and physical cues without any chemical modification of polymer backbone or additional polymer processing techniques to create synthetic ECM analogues. This approach allows noninteracting modification of multiple niche properties (e.g., bioactive ligands, stiffness, porosity), since no covalent conjugation method was used to modify PEG monomers for incorporation of bioactivity and porosity. Combining the self-assembled PA nanofibers with a chemically cross-linked polymer network simply by facile mixing followed by photopolymerization resulted in the formation of porous bioactive hydrogel systems. The resulting porous network can be functionalized with desired bioactive signaling epitopes by simply altering the amino acid sequence of the self-assembling PA molecule. In addition, the mechanical properties of the composite system can be precisely controlled by changing the PEG concentration. Therefore, nanofibrous self-assembled PA/ PEG composite hydrogels reported in this work can provide new opportunities as versatile synthetic mimics of ECM with independently tunable biological and mechanical properties for tissue engineering and regenerative medicine applications. In addition, such systems could provide useful tools for investigation of how complex niche cues influence cellular behavior and tissue formation both in two-dimensional and three-dimensional platforms.

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“…This technique, in which a polymer solution is deposited into highly interconnected templates of nanofibers via an electrical field, has many advantages with regard to tissue engineering. Characteristics such as fiber diameter and pore size can be tailored by optimizing the polymer solution flow rate, air-gap distance between the extrusion needle and collecting plate, concentration of polymer in the solution, and the voltage applied to the needle tip [3,4]. Furthermore, a wide variety of natural and synthetic polymers can be used, allowing tailorability of degradation method (hydrolytic degradation or enzymatic biodegradation), degradation timeframe, template mechanical properties, and biocompatibility [5,6].…”

Section: Introductionmentioning

confidence: 99%

Electrospun fibers/branched-clusters as building units for tissue engineering

Minden-Birkenmaier¹,

Selders²,

Cherukuri³

et al. 2017

Electrospinning

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Although electrospun templates are effective at mimicking the extracellular matrix (ECM) of native tissue due to the tailorability of parameters such as fiber diameter, polymer composition, and drug loading, these templates are often limited with regards to cell infiltration and the tailorability of the microenvironments within the structures. Thus, there remains a need for a flexible threedimensional template system which could be combined with cell suspensions to promote three-dimensional tissue regeneration, and ultimately allow cells to freely reorganize and modify their microenvironment. In this study, a mincing process was designed and optimized to create mixtures of electrospun fibers/branched-clusters for use as fundamental tissue engineering building units. These fiber/branched-cluster elements were characterized with regards to fiber and branch lengths, and a method was optimized to combine them with normal human dermal fibroblasts (nHDFs) in culture to create interconnected template constructs. Sectioning and imaging of these constructs revealed cell/fiber integration as well as even cell distribution throughout the construct interior. These fiber/branched-cluster elements represent an innovative flexible tissue regeneration template system.

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Tailoring the porosity and pore size of electrospun synthetic human elastin scaffolds for dermal tissue engineering

Cited by 280 publications

References 46 publications

Intermolecular Interactions and the Release Pattern of Electrospun Curcumin-Polyvinyl(pyrrolidone) Fiber

Intermolecular Interactions and the Release Pattern of Electrospun Curcumin-Polyvinyl(pyrrolidone) Fiber

Self-Assembled Peptide Amphiphile Nanofibers and PEG Composite Hydrogels as Tunable ECM Mimetic Microenvironment

Electrospun fibers/branched-clusters as building units for tissue engineering

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