Tailoring Tissue Engineering Scaffolds Using Electrostatic Processing Techniques: A Study of Poly(glycolic Acid) Electrospinning

Boland, Eugene D.; Wnek, Gary E.; Simpson, David G.; Pawlowski, Kristin J.; Bowlin, Gary L.

doi:10.1081/ma-100108380

Cited by 415 publications

(188 citation statements)

References 3 publications

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“…collagen, silk fibroin, and fibrinogen) and synthetic polymers (e.g. PGA, PLLA, PLGA, and PCL) have been processed into fine nonwoven fabrics for tissue engineering research [48,[50][51][52][53][54][55][56][57][58][59][60]. Various cells have been reported to attach, proliferate, and differentiate into or maintain their functional phenotypes on these electrospun nano-fibrous materials [48,54,58,59,61,62].…”

Section: Electrospinningmentioning

confidence: 99%

Biomimetic materials for tissue engineering

Peter

2008

Advanced Drug Delivery Reviews

1,212

819

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Tissue engineering and regenerative medicine is an exciting research area that aims at regenerative alternatives to harvested tissues for transplantation. Biomaterials play a pivotal role as scaffolds to provide three-dimensional templates and synthetic extracellular-matrix environments for tissue regeneration. It is often beneficial for the scaffolds to mimic certain advantageous characteristics of the natural extracellular matrix, or developmental or would healing programs. This article reviews current biomimetic materials approaches in tissue engineering. These include synthesis to achieve certain compositions or properties similar to those of the extracellular matrix, novel processing technologies to achieve structural features mimicking the extracellular matrix on various levels, approaches to emulate cell-extracellular matrix interactions, and biologic delivery strategies to recapitulate a signaling cascade or developmental/would-healing program. The article also provides examples of enhanced cellular/tissue functions and regenerative outcomes, demonstrating the excitement and significance of the biomimetic materials for tissue engineering and regeneration.

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Section: Electrospinningmentioning

confidence: 99%

Biomimetic materials for tissue engineering

Peter

2008

Advanced Drug Delivery Reviews

1,212

819

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“…method for aligning fibers for the generation of tissue engineering scaffolds (and the method that our lab uses) is to deposit fibers onto a rotating drum or mandrel. [78][79][80][81][82][83][84] From high-speed imaging studies, it has been shown that a single fiber can transit from the source spinneret to the ground at speeds >2 m=s. 85 Thus, presenting a surface moving faster than the fiber jet will pull fibers into alignment as they are deposited.…”

Section: Figmentioning

confidence: 99%

Engineering on the Straight and Narrow: The Mechanics of Nanofibrous Assemblies for Fiber-Reinforced Tissue Regeneration

Mauck

Baker

Nerurkar

et al. 2009

Tissue Engineering Part B: Reviews

191

177

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Tissue engineering of fibrous tissues of the musculoskeletal system represents a considerable challenge because of the complex architecture and mechanical properties of the component structures. Natural healing processes in these dense tissues are limited as a result of the mechanically challenging environment of the damaged tissue and the hypocellularity and avascular nature of the extracellular matrix. When healing does occur, the ordered structure of the native tissue is replaced with a disorganized fibrous scar with inferior mechanical properties, engendering sites that are prone to re-injury. To address the engineering of such tissues, we and others have adopted a structurally motivated approach based on organized nanofibrous assemblies. These scaffolds are composed of ultrafine polymeric fibers that can be fabricated in such a way to recreate the structural anisotropy typical of fiber-reinforced tissues. This straight-and-narrow topography not only provides tailored mechanical properties, but also serves as a 3D biomimetic micropattern for directed tissue formation. This review describes the underlying technology of nanofiber production and focuses specifically on the mechanical evaluation and theoretical modeling of these structures as it relates to native tissue structure and function. Applying the same mechanical framework for understanding native and engineered fiber-reinforced tissues provides a functional method for evaluating the utility and maturation of these unique engineered constructs. We further describe several case examples where these principles have been put to test, and discuss the remaining challenges and opportunities in forwarding this technology toward clinical implementation.

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“…Hence, extensive studies in recent years have evaluated the potentials of polymeric nanofibrous structures as tissue engineering scaffolds in biomedical applications (Matthews et al 2002;Yoshimoto et al 2003). A variety of polymers have been electrospun as the scaffolds and various applications have been proposed including skin, cartilage, bone and blood vessel tissue engineering (Boland et al 2001;Huang et al 2001;Stitzel et al 2001;Li et al 2002;Matthews et al 2002;Yoshimoto et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Long-term viability of coronary artery smooth muscle cells on poly( l -lactide- co -ϵ-caprolactone) nanofibrous scaffold indicates its potential for blood vessel tissue engineering

Dong

Tan

Liao

et al. 2008

J. R. Soc. Interface.

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Biodegradable polymer nanofibres have been extensively studied as cell culture scaffolds in tissue engineering. However, long-term in vitro studies of cell-nanofibre interactions were rarely reported and successful organ regeneration using tissue engineering techniques may take months (e.g. blood vessel tissue engineering). Understanding the long-term interaction between cells and nanofibrous scaffolds (NFS) is crucial in material selection, design and processing of the tissue engineering scaffolds. In this study, poly(L-lactide-co-3-caprolactone) [P(LLA-CL)] (70 : 30) copolymer NFS were produced by electrospinning. Porcine coronary artery smooth muscle cells (PCASMCs) were seeded and cultured on the scaffold to evaluate cell-nanofibre interactions for up to 105 days. A favourable interaction between this scaffold and PCASMCs was demonstrated by cell viability assay, scanning electron microscopy, histological staining and extracellular matrix (ECM) secretion. Degradation behaviours of the scaffolds with or without PCASMC culture were determined by mechanical testing and gel permeation chromatography (GPC). The results showed that the PCASMCs attached and proliferated well on the P(LLA-CL) NFS. Large amount of ECM protein secretion was observed after 50 days of culture. Multilayers of aligned oriented PCASMCs were formed on the scaffold after two months of in vitro culture. In the degradation study, the PCASMCs were not shown to significantly increase the degradation rate of the scaffolds for up to 105 days of culture. The in vitro degradation time of the scaffold could be as long as eight months by extrapolating the results from GPC. These observations further supported the potential use of the P(LLA-CL) nanofibre in blood vessel tissue engineering.

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Tailoring Tissue Engineering Scaffolds Using Electrostatic Processing Techniques: A Study of Poly(glycolic Acid) Electrospinning

Cited by 415 publications

References 3 publications

Biomimetic materials for tissue engineering

Biomimetic materials for tissue engineering

Engineering on the Straight and Narrow: The Mechanics of Nanofibrous Assemblies for Fiber-Reinforced Tissue Regeneration

Long-term viability of coronary artery smooth muscle cells on poly( l -lactide- co -ϵ-caprolactone) nanofibrous scaffold indicates its potential for blood vessel tissue engineering

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