Talaromyces columbinus sp. nov., and Genealogical Concordance Analysis in Talaromyces Clade 2a

Abstract

During the course of mold surveys, a set of Talaromyces isolates were obtained that did not fit any described species. Phenotypic examination of these isolates showed that they were similar to T. piceus but differed in some growth characteristics. Multilocus DNA sequence data were obtained for the new isolates and some related species in the broader, more inclusive clade, and the data were analyzed using genealogical concordance. The new isolates are described as Talaromyces columbinus. From analysis of the related species, Penicillium rugulosum var. atricolum is given species status in Talaromyces as T. atricola. Penicillium tardum and P. chrysitis were showed to be synonyms of T. rugulosus. Penicillium scorteum and T. phialosporus were showed to be conspecific and under the rule of priority T. scorteus is the proper name for isolates previously known as T. phialosporus. Talaromyces wortmanii was showed to be distinct from Penicillium concavorugulosum and T. variabilis but the relationship of the latter two species remains unresolved. Examination of ITS sequences from GenBank showed that T. columbinus has previously been reported from human lung infections under the name Penicillium piceum.

Aspergillus asper sp. nov. and Aspergillus collinsii sp. nov., from Aspergillus section Usti

“…Cultures were grown in duplicate as three-point inoculations in 9 cm diameter Petri dishes. Fungal isolation and microscopy were performed as described by Peterson & Jurjević (2013).…”

“…DNA was extracted from freeze-dried mycelium using the CTAB method (Peterson et al, 2015). Beta tubulin (BT2), calmodulin (CF), internal transcribed spacer region (ITS; containing ITS1, 5.8S rDNA and ITS2) and DNA-dependent RNA polymerase II second largest subunit (RPB2) were amplified from genomic DNA and sequenced using published methods (Peterson & Jurjević , 2013). Phylogenetic analysis of data sets from each locus was carried out with MEGA 6.06 (Tamura et al, 2013) using maximum-likelihood criterion and the model determined using the built-in model selection test routine.…”

Influence of Harvest Timing, Fungicides, and Beet necrotic yellow vein virus on Sugar Beet Storage

“…Because not all the DNA regions were informative for differentiating all the fungal species observed, the DNA regions sequenced for each mycelial type were chosen based on previous phylogenetic analyses from taxonomic studies for the fungal genera observed (i.e., Athelia-like, Botrytis, Fusarium, Mucor, Neonectria, Penicillium, Phoma, Rhizopus, Sarocladium, and Talaromyces spp. ; [3][4][5]18,20,21,25,35,39,43,53,55,59,63). The isolates were each grown in potato dextrose broth (PDB; Becton Dickinson & Co.) on a DS-500E orbital shaker (VWR International, LLC, Aurora, CO; 100 rpm) at 21°C until a ball of mycelium (approximately 10 mm in diameter) was generated from the original 5-mm-diameter agar plug.…”

Clinical, Morphological, and Molecular Characterization of Penicilliumcanissp. nov., Isolated from a Dog with Osteomyelitis

“…1 paper, placed in microcentrifuge tubes, frozen, and freeze-dried. Dry mycelium was ground to powder and rehydrated with cetyltrimethylammonium bromide buffer, proteins were extracted with chloroform, and nucleic acids were precipitated with isopropanol (27).…”

“…␤-Tubulin (BT2), calmodulin (CF), the nuclear ITS region, minichromosome maintenance factor (Mcm7), DNA-dependent RNA polymerase (RPB2), and pre-rRNA processing protein (Tsr1) were amplified with the primers and under the conditions specified by Peterson and Jurjevic (27). Amplified DNA was prepared for sequencing with ExoSapit (Affymetrix), sequencing reactions were carried out with BigDye ver.…”