Talin1 regulates glucose metabolism and endometrial receptivity
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            GLUT-4 in patients with polycystic ovary syndrome and insulin resistance

Li, Jingjing; Chen, Saiqiong; Qin, Rongyan; Liu, Xin; Li, Fan; Wei, Minggang; Wei, Jiajia; Lin, Jiajing; Zheng, Fengque

doi:10.1080/09513590.2023.2231085

Cited by 4 publications

(3 citation statements)

References 35 publications

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“…However, there is no clear correlation between AMH concentration and insulin resistance. One Chinese study found that AMH is a valuable prognostic marker for insulin resistance [22].…”

Section: Introductionmentioning

confidence: 99%

Ovarian Factors of Cardiovascular Disease: The Way to Go?

V. Lakhno

2024

Women's Health Problems - A Global Perspective

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The function of the female reproductive system is critically dependent on the general health of a woman. However, the activity of ovaries has its projection on metabolic processes, vascular tone, and endothelial function. Hyperandrogenicity in polycystic ovarian disease is a trigger for adiposity, diabetes type II, and atherogenic vasculopathy. The increased level of testosterone persists to stay even after menopause. The data on hormonal changes during menopause is discussed. Hormone replacement therapy did not demonstrate an evident beneficial effect on the cardiovascular system. The possible therapeutic strategy for improved cardiovascular health during the transitional year of life is presented. The use of diet, L-arginine, and xylitol could be a reasonable option in the management of perimenopausal women.

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“…However, there is no clear correlation between AMH concentration and insulin resistance. One Chinese study found that AMH is a valuable prognostic marker for insulin resistance [22].…”

Section: Introductionmentioning

confidence: 99%

Ovarian Factors of Cardiovascular Disease: The Way to Go?

V. Lakhno

2024

Women's Health Problems - A Global Perspective

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“…Genetic differences have been identified as a contributing factor to the pathophysiology of PCOS in many investigations [3,4]. Furthermore, it has been shown that there is a correlation between insulin resistance and the pathophysiology of PCOS [5,6]. How chronic low-level inflammation might be involved in PCOS pathogenesis has been the subject of scientific inquiry [7,8].…”

Section: Introductionmentioning

confidence: 99%

Ghrelin Alleviates Inflammation, Insulin Resistance, and Reproductive Abnormalities in Mice with Polycystic Ovary Syndrome via the TLR4-NF-κB Signaling Pathway

Liu,

Wang,

Zhao

et al. 2024

Discovery Medicine

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Background: Polycystic ovary syndrome (PCOS) commonly impacts fertile females with potentially severe effects on fertility and metabolism. Blood ghrelin levels are lower in PCOS patients, and exogenous supplements have been proposed for their potential to trigger anti-inflammatory effects at the cellular level. This study aimed to investigate whether pretreatment with ghrelin reduced inflammation, insulin resistance, and reproductive abnormalities in PCOS and the underlying mechanism of this disorder. Methods: Ghrelin supplementation was first tested in an inflammation model using human ovarian granulosa cells (KGN cells) that were built by treated with Lipolyaccharide. KGN cells were pretreated with ghrelin and exposed to lipopolysaccharide (LPS). Inflammatory gene expression and cytokine production were analyzed by Enzyme-linked immunosorbent assay (ELISA). Based on these results, the PCOS mice model was built with Dehydroepiandrosterone (DHEA) and a high-fat diet. The mRNA and protein expressions of inflammatory factors including Toll-like receptor 4 (TLR4), nuclear factor kappa-B-p65 (NF-κB-p65), Phospho-NF-κB-p65 (p-NF-κB-p65) and myeloid differentiation factor 88 (MYD88) related to the TLR4/NF-κB signaling pathway were evaluated in KGN cells and mouse ovarian tissues using Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) and western blot, respectively. Lipid metabolism was quantified via an automated biochemical analyzer. Results: The mRNA and protein expressions of interleukin-6 (IL-6), interleukin-1 beta (IL-1β), and tumor necrosis factor alpha (TNF-α) in ghrelin pretreated KGN cells were lower than the LPS group (p < 0.05). Protein expression was reduced for TLR4, NF-κB-p65, and MYD88 within KGN cells of ghrelin groups compared to the LPS group (p < 0.05). Ghrelin treatment restored the estrous cycle and slowed weight gain and abdominal fat weight of PCOS mice (p < 0.05). Ghrelin treatment decreased the serum concentrations of testosterone, luteinizing hormone, insulin, IL-6, IL-1β, and TNF-α compared to the PCOS group (p < 0.05). Estradiol concentrations of mice treated with ghrelin were higher than the PCOS group (p < 0.05). The concentrations of low and high-density lipoprotein, triglyceride, and cholesterol in mice treated with ghrelin were lower than in the PCOS mice (p < 0.05). Inflammatory gene expression for IL-6, IL-1β, TNF-α, TLR4, NF-κB-p65, and MYD88 decreased in the ovarian tissues of ghrelin-treated mice compared to the PCOS group (p < 0.05), along with reduced protein expression of TLR4, p-NF-κB-p65, and MYD88 (p < 0.05). Conclusions: In the present study, ghrelin treatment effectively reduced inflammation in vitro, and attenuated insulin resistance and reproductive abnormalities in PCOS mice through the TLR4/NF-κB signaling pathway, highlighting potential therapeutic avenues for future PCOS treatments and research directions.

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“…IR appears to be a key factor PCOS development, with the ovary being only one among several organs affected ( 11 ). Nearly 44–70% of women diagnosed with PCOS have IR with endometrial IR and impaired glucose transport, leading to chronic low-grade inflammation, immune dysfunction modifications in the vascular uterus, elevated endometrial gene expression, and cellular abnormalities ( 12 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular role of non-exonic variants in CALPAIN 10 gene in polycystic ovarian syndrome in Saudi women

Alageel,

Alshammary,

Ali Khan

2023

Front. Endocrinol.

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IntroductionNon-diabetic women with polycystic ovarian syndrome (PCOS) often have abnormal insulin regulation. Calpain 10 (CALP10) is a biomarker of type 2 diabetes mellitus, with some of its single-nucleotide polymorphisms (SNPs) influencing PCOS development.MethodsIn this case-control study on 90 women each with and without PCOS, we explored the molecular role of five CALP10 SNPs using biochemical parameters and Sanger sequencing analyses.ResultsDifferent genetic models, genotypes, and allele frequencies were significantly associated with UCSNP-19 (rs3842570; p=0.01), UCSNP-44 (rs2975760; p=0.009), UCSNP-56 (rs2975762; p<0.0001), and UCSNP-63 (rs5030952; p=0.0003) in women with PCOS. The multiple logistic regression model showed a strong association of CALP10 SNPs with fasting blood glucose (p<0.001). ANOVA showed significant associations with various biochemical parameters such as FSH (p=0.0001) in UCSNP-19 (rs3842570), FI (p=0.002), TG (p=0.01) in UCSNP-56 (rs2975762) and FBG (p=0.001), FI (p=0.004), FSH (p=0.02) & LDLc (p=0.04) in UCSNP-63 (rs5030952) SNPs. Haplotype analysis also revealed significant associations between different combinations of alleles in the studied 5 SNPs in women with PCOS (p<0.05). Generalized multifactor dimensionality reduction analysis showed the best gene–gene interactions among the five SNPs in CALP10I (p<0.05). However, dendrogram and graphical depletion models found no strong association in women with PCOS.ConclusionIn conclusion, this study confirms rs3842570, rs2975760, rs2975767, and rs5030952 SNPs in CALP10 gene is associated in diagnosed PCOS women in the Saudi Arabia.

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Talin1 regulates glucose metabolism and endometrial receptivity via GLUT-4 in patients with polycystic ovary syndrome and insulin resistance

Cited by 4 publications

References 35 publications

Ovarian Factors of Cardiovascular Disease: The Way to Go?

Ovarian Factors of Cardiovascular Disease: The Way to Go?

Ghrelin Alleviates Inflammation, Insulin Resistance, and Reproductive Abnormalities in Mice with Polycystic Ovary Syndrome via the TLR4-NF-κB Signaling Pathway

Molecular role of non-exonic variants in CALPAIN 10 gene in polycystic ovarian syndrome in Saudi women

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