Talking to your neighbors across scales: Long-distance Notch signaling during patterning

Hadjivasiliou, Zena; Hunter, Ginger L.

doi:10.1016/bs.ctdb.2022.04.002

Cited by 5 publications

(5 citation statements)

References 119 publications

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“…Theoretical and experimental data demonstrate that molecular modifiers of Notch signaling and cell morphology play roles in the density of the final bristle pattern. Models clearly support a characteristic length for the lateral inhibition signal beyond adjacent cells, which could be achieved by active cell processes and by diffusible signals (Cohen et al, 2010;Corson et al, 2017;Hadjivasiliou et al, 2016;Hadjivasiliou and Hunter, 2022). Dynamic actin-based signaling filopodia, or cytonemes, have been shown to play a role in the Notch response of epithelial cells during patterning stages (Cohen et al, 2010;Georgiou and Baum, 2010;Hunter et al, 2019).…”

Section: Introductionmentioning

confidence: 85%

Scabrous is distributed via signaling filopodia to modulate Notch response during bristle patterning in Drosophila

Presser

Freund

Hassapelis

et al. 2023

Preprint

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During development, cells in tissues must be patterned correctly in order to support tissue function and shape. The sensory bristles of the peripheral nervous system on the thorax of Drosophila melanogaster self-organizes from a unpatterned epithelial tissue to a regular spot pattern during pupal stages. Wild type patterning requires Notch-mediated lateral inhibition. Scabrous is a protein that can bind to and modify Notch receptor activity. Scabrous can be secreted, but it is also known to be localized to basal signaling filopodia, or cytonemes, that play a role in long-range Notch signaling. Consistent with previous work, we show that Scabrous is primarily distributed basally, within the range of signaling filopodia extension. We show that signaling filopodia are required for the distribution of Scabrous protein during sensory bristle patterning stages. We show that the Notch response of epithelial cells is sensitive to the level of Scabrous protein being expressed by the sensory bristle precursor cell. Our findings at the cell-level suggest a model for how epithelial cells engaged in lateral inhibition at a distance are sensitive local levels of Scabrous protein.

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Section: Introductionmentioning

confidence: 85%

Scabrous is distributed via signaling filopodia to modulate Notch response during bristle patterning in Drosophila

Presser

Freund

Hassapelis

et al. 2023

Preprint

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show abstract

“…The signalling, pathways involving paracrine and juxtacrine systems comprise an extensive network that intricately governs cellular behaviours and interactions, notably in the microenvironment of stem cell regulation [ 39 , 40 ]. Paracrine signalling is the production of signalling molecules by one cell to target adjacent cells, altering stem cell activity such as proliferation and differentiation by use of growth factors and cytokines [ 41 ].…”

Section: Modern Regenerative Therapies Using Three-dimensional Organoidsmentioning

confidence: 99%

“…While in 3D culture the is increased cell to cell contact and increased spatial organization allowing for increased diffusion of paracrine factors as well as prompting cell-to-cell communication [ 44 ]. The juxtacrine signalling mechanism by which direct cell-to-cell contact and signalling is facilitated increases in 3D culture as there is an increase in the spatial organization and promotes ligand-receptor interaction allowing for a direct signalling pathway to exist between the cells [ 40 , 43 ]. In 3D culture system there is an increase in the pathway involving the paracrine and juxtacrine signalling due to increase in cell-to-cell communication and reduced diffusion distance.…”

Section: Modern Regenerative Therapies Using Three-dimensional Organoidsmentioning

confidence: 99%

Insights on Three Dimensional Organoid Studies for Stem Cell Therapy in Regenerative Medicine

Mulaudzi,

Abrahamse,

Crous

2023

Stem Cell Rev and Rep

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Regenerative medicine has developed as a promising discipline that utilizes stem cells to address limitations in traditional therapies, using innovative techniques to restore and repair damaged organs and tissues. One such technique is the generation of three-dimensional (3D) organoids in stem cell therapy. Organoids are 3D constructs that resemble specific organs' structural and functional characteristics and are generated from stem cells or tissue-specific progenitor cells. The use of 3D organoids is advantageous in comparison to traditional two-dimensional (2D) cell culture by bridging the gap between in vivo and in vitro research. This review aims to provide an overview of the advancements made towards regenerative medicine using stem cells to generate organoids, explore the techniques used in generating 3D organoids and their applications and finally elucidate the challenges and future directions in regenerative medicine using 3D organoids. Graphical Abstract

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“…Theoretical and experimental data demonstrate that molecular modifiers of Notch signaling and cell morphology play roles in the density of the final bristle pattern [ 4 , 11 , 12 ]. Models clearly support a characteristic length for the lateral inhibition signal beyond adjacent cells, which could be achieved by active cell processes and by diffusible signals [ 13 ]. Dynamic actin-based signaling filopodia, or cytonemes, have been shown to play a role in the Notch response of epithelial cells during patterning stages [ 4 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Scabrous is distributed via signaling filopodia to modulate Notch response during bristle patterning in Drosophila

Presser,

Freund,

Hassapelis

et al. 2023

PLoS ONE

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During development, cells in tissues must be patterned correctly in order to support tissue function and shape. The sensory bristles of the peripheral nervous system on the thorax of Drosophila melanogaster self-organizes from a unpatterned epithelial tissue to a regular spot pattern during pupal stages. Wild type patterning requires Notch-mediated lateral inhibition. Scabrous is a protein that can bind to and modify Notch receptor activity. Scabrous can be secreted, but it is also known to be localized to basal signaling filopodia, or cytonemes, that play a role in long-range Notch signaling. Here we show that Scabrous is primarily distributed basally, within the range of signaling filopodia extension. We show that filamentous actin dynamics are required for the distribution of Scabrous protein during sensory bristle patterning stages. We show that the Notch response of epithelial cells is sensitive to the level of Scabrous protein being expressed by the sensory bristle precursor cell. Our findings at the cell-level suggest a model for how epithelial cells engaged in lateral inhibition at a distance are sensitive local levels of Scabrous protein.

show abstract

Talking to your neighbors across scales: Long-distance Notch signaling during patterning

Cited by 5 publications

References 119 publications

Scabrous is distributed via signaling filopodia to modulate Notch response during bristle patterning in Drosophila

Scabrous is distributed via signaling filopodia to modulate Notch response during bristle patterning in Drosophila

Insights on Three Dimensional Organoid Studies for Stem Cell Therapy in Regenerative Medicine

Scabrous is distributed via signaling filopodia to modulate Notch response during bristle patterning in Drosophila

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