2023
DOI: 10.21203/rs.3.rs-2404996/v1
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TaME-seq2: Tagmentation-assisted multiplex PCR enrichment sequencing for viral genomic profiling

Abstract: Background Previously developed TaME-seq method for deep sequencing of HPV, allowed simultaneous identification of the HPV DNA consensus sequence, low-frequency variable sites, and chromosomal integration events. The method has been successfully validated and applied to the study of five carcinogenic high-risk (HR) HPV types (HPV16, 18, 31, 33, and 45). Here, we present TaME-seq2 with an updated laboratory workflow and bioinformatics pipeline. The HR-HPV type repertoire was expanded with HPV51, 52, and 59. As … Show more

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