TaMYC8 regulates TaERF6 and inhibits ethylene synthesis to confer Cd tolerance in wheat

Wang, Hongcheng; Zuo, Dan; Zhu, Bin; Du, Xuye; Gu, Lei

doi:10.1016/j.envexpbot.2022.104854

Cited by 14 publications

(4 citation statements)

References 60 publications

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“…B3 and SI-IAA27-SI-NSP1 [64]. Moreover, overexpressing ApHIPP26 inhibited the activity of the ethylene pathway, in which three genes and the key gene in ethylene synthesis, ACS2, were all downregulated, which is consistent with the results showing that inhibiting ethylene synthesis can enhance cadmium tolerance in plants [65,66]. In our study, AtIAA27 (AT4G29080) in auxin and AtERF1 (AT3G23240) in the ethylene pathway were differentially expressed.…”

Section: Discussionsupporting

confidence: 90%

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Overexpression of ApHIPP26 from the Hyperaccumulator Arabis paniculata Confers Enhanced Cadmium Tolerance and Accumulation to Arabidopsis thaliana

Zhou,

Ye,

Pang

et al. 2023

IJMS

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Cadmium (Cd) is a toxic heavy metal that seriously affects metabolism after accumulation in plants, and it also causes adverse effects on humans through the food chain. The HIPP gene family has been shown to be highly tolerant to Cd stress due to its special domain and molecular structure. This study described the Cd-induced gene ApHIPP26 from the hyperaccumulator Arabis paniculata. Its subcellular localization showed that ApHIPP26 was located in the nucleus. Transgenic Arabidopsis overexpressing ApHIPP26 exhibited a significant increase in main root length and fresh weight under Cd stress. Compared with wild-type lines, Cd accumulated much more in transgenic Arabidopsis both aboveground and underground. Under Cd stress, the expression of genes related to the absorption and transport of heavy metals underwent different changes in parallel, which were involved in the accumulation and distribution of Cd in plants, such as AtNRAMP6 and AtNRAMP3. Under Cd stress, the activities of antioxidant enzymes (superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase) in the transgenic lines were higher than those in the wild type. The physiological and biochemical indices showed that the proline and chlorophyll contents in the transgenic lines increased significantly after Cd treatment, while the malondialdehyde (MDA) content decreased. In addition, the gene expression profile analysis showed that ApHIPP26 improved the tolerance of Arabidopsis to Cd by regulating the changes of related genes in plant hormone signal transduction pathway. In conclusion, ApHIPP26 plays an important role in cadmium tolerance by alleviating oxidative stress and regulating plant hormones, which provides a basis for understanding the molecular mechanism of cadmium tolerance in plants and provides new insights for phytoremediation in Cd-contaminated areas.

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Section: Discussionsupporting

confidence: 90%

“…After three weeks in Hoagland solution, A. paniculata seedlings were treated with 0.25 mM CdCl 2 for seven days. The artificial climate incubator strictly implements the light and humidity control system with specific indicators referring to a published article [66].…”

Section: Plant Culture and Treatmentsmentioning

confidence: 99%

Overexpression of ApHIPP26 from the Hyperaccumulator Arabis paniculata Confers Enhanced Cadmium Tolerance and Accumulation to Arabidopsis thaliana

Zhou,

Ye,

Pang

et al. 2023

IJMS

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“…The ERF TFs regulates stress responses linked to ethylene, a significant stress hormone induced by various abiotic factors ( Wang et al., 2022a ). ERF TFs were initially identified due to their ability to bind to promoters of stress response genes.…”

Section: Erf Tfs Regulate Plant CD Stress Responses By Modulating Ros...mentioning

confidence: 99%

Transcription factors involved in plant responses to cadmium-induced oxidative stress

Zhang,

2024

Front. Plant Sci.

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Cadmium (Cd) is a heavy metal highly toxic to living organisms. Cd pollution of soils has become a serious problem worldwide, posing a severe threat to crop production and human health. When plants are poisoned by Cd, their growth and development are inhibited, chloroplasts are severely damaged, and respiration and photosynthesis are negatively affected. Therefore, elucidating the molecular mechanisms that underlie Cd tolerance in plants is important. Transcription factors can bind to specific plant cis-acting genes. Transcription factors are frequently reported to be involved in various signaling pathways involved in plant growth and development. Their role in the resistance to environmental stress factors, particularly Cd, should not be underestimated. The roles of several transcription factor families in the regulation of plant resistance to Cd stress have been widely demonstrated. In this review, we summarize the mechanisms of five major transcription factor families–WRKY, ERF, MYB, bHLH, and bZIP–in plant resistance to Cd stress to provide useful information for using molecular techniques to solve Cd pollution problems in the future.

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“…GmbHLH3 was found to interact with the G-box cis-acting element in the GmCLC1 promoter, regulating its activation in response to chlorine (Cl − ) stress/salinity [19]. TaMYC8, a member of the bHLH superfamily, directly binds to the G-box in the TaERF6 promoter, thereby regulating the Cd stress response [37]. In this study, the SbbHLH041 protein was found to directly bind to the E-box in the P3 (−1972~−1945) fragment in the SbEXPA11 promoter (Figure 2b,c), indicating that SbbHLH041 targets SbEXPA11 by specifically binding to the E-box of its promoter.…”

Section: The Sbbhlh041 Tf Activates Sbexpa11 Expression By Directly B...mentioning

confidence: 99%

The SbbHLH041–SbEXPA11 Module Enhances Cadmium Accumulation and Rescues Biomass by Increasing Photosynthetic Efficiency in Sorghum

Wang,

Yu,

Zhu

et al. 2023

IJMS

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In plants, expansin genes are responsive to heavy metal exposure. To study the bioremediary potential of this important gene family, we discovered a root-expressed expansin gene in sorghum, SbEXPA11, which is notably upregulated following cadmium (Cd) exposure. However, the mechanism underlying the Cd detoxification and accumulation mediated by SbEXPA11 in sorghum remains unclear. We overexpressed SbEXPA11 in sorghum and compared wild-type (WT) and SbEXPA11-overexpressing transgenic sorghum in terms of Cd accumulation and physiological indices following Cd. Compared with the WT, we found that SbEXPA11 mediates Cd tolerance by exerting reactive oxygen species (ROS)-scavenging effects through upregulating the expression of antioxidant enzymes. Moreover, the overexpression of SbEXPA11 rescued biomass production by increasing the photosynthetic efficiency of transgenic plants. In the pot experiment with a dosage of 10 mg/kg Cd, transgenic sorghum plants demonstrated higher efficacy in reducing the Cd content of the soil (8.62 mg/kg) compared to WT sorghum plants (9.51 mg/kg). Subsequent analysis revealed that the SbbHLH041 transcription factor has the ability to induce SbEXPA11 expression through interacting with the E-box located within the SbEXPA11 promoter. These findings suggest that the SbbHLH041–SbEXPA11 cascade module may be beneficial for the development of phytoremediary sorghum varieties.

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TaMYC8 regulates TaERF6 and inhibits ethylene synthesis to confer Cd tolerance in wheat

Cited by 14 publications

References 60 publications

Overexpression of ApHIPP26 from the Hyperaccumulator Arabis paniculata Confers Enhanced Cadmium Tolerance and Accumulation to Arabidopsis thaliana

Overexpression of ApHIPP26 from the Hyperaccumulator Arabis paniculata Confers Enhanced Cadmium Tolerance and Accumulation to Arabidopsis thaliana

Transcription factors involved in plant responses to cadmium-induced oxidative stress

The SbbHLH041–SbEXPA11 Module Enhances Cadmium Accumulation and Rescues Biomass by Increasing Photosynthetic Efficiency in Sorghum

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