Tandem arrayed Iigation of expressed sequence tags (TALEST): a new method for generating global gene expression profiles

Spinella, Dominic G.; Bernardino, Alexandra; Redding, A. C.; Koutz, P J; Yue, Wei; Pratt, Ellen K.; Myers, Kristi K.; Chappell, Gary; Gerken, Steven C.; McConnell, Stephen J.

doi:10.1093/nar/27.18.e22-i

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“…Comparing to the original SAGE protocol yielding variable 10-11 bp tags (plus 4 bp for a residue of anchoring enzyme sequence), MAGE [35] and tandem arrayed ligation of expressed sequence tags (TALEST, [54]) are able to yield 12 bp tags (plus 5 bp for a residue of anchoring enzyme sequence for MAGE; plus 4 bp for a residue of punctuating enzyme sequence for TALEST). Moreover, another SAGE modification by A. Ryo and coauthors [31] yields 14 bp tags (plus 2 bp for a residue of anchoring enzyme sequence; an alternative RsaI enzyme have been utilized), while longSAGE [42], aRNA-longSAGE [55], Robust-Long SAGE (RL-SAGE, [56]) and deepSAGE [57] protocols all yield even longer, 17-18 bp tags (plus 4 bp for residues of anchoring enzyme sequences).…”

Section: Further Development Of Sage Techno-logymentioning

confidence: 99%

Serial Analysis of Gene Expression (SAGE): 13 Years of Application in Research

Anisimov¹

2008

CPB

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A number of molecular methods of gene expression analysis can approach genomic level. Among those, Serial Analysis of Gene Expression (SAGE) stands out. Unlike many other techniques, SAGE allows both qualitative and quantitative analysis of previously unknown transcripts. Over the course of the last 13 years, SAGE has became a recognized tool of large-scale gene expression profiling, being used extensively in human, animal, yeast and plant studies of various nature. A number of important adaptations was introduced both to the protocol of SAGE library construction and to the analytical algorithm employed. Moreover, some variations of the original protocol (MAGE, SADE, microSAGE, miniSAGE, longSAGE, superSAGE, deepSAGE, etc.) were derived to improve the utility of SAGE in certain conditions. Current review aims comparing the benefits and drawbacks of the techniques for high-throughput gene expression analysis (including SAGE) in a realistic, balanced manner. Issues related to modifications to the original protocol and further development of the SAGE are discussed.

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Section: Further Development Of Sage Techno-logymentioning

confidence: 99%

Serial Analysis of Gene Expression (SAGE): 13 Years of Application in Research

Anisimov¹

2008

CPB

View full text Add to dashboard Cite

show abstract

Tandem arrayed Iigation of expressed sequence tags (TALEST): a new method for generating global gene expression profiles

Cited by 1 publication

References 11 publications

Serial Analysis of Gene Expression (SAGE): 13 Years of Application in Research

Serial Analysis of Gene Expression (SAGE): 13 Years of Application in Research

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