Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440

Werner, Allison Z.; Clare, Rita; Mand, Thomas D.; Pardo, Isabel; Ramirez, Kelsey J.; Haugen, Stefan J.; Bratti, Felicia; Dexter, Gara N.; Elmore, Joshua R.; Huenemann, Jay D.; Peabody, George L.; Johnson, Christopher W.; Rorrer, Nicholas A.; Salvachúa, Davinia; Guss, Adam M.; Beckham, Gregg T.

doi:10.1016/j.ymben.2021.07.005

Cited by 107 publications

(102 citation statements)

References 64 publications

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“…DK17 ( 11 ), Rhodococcus jostii RHA1 ( 14 ), Pseudomonas umsongensis GO16 ( 8 ), and Acinetobacter baylyi ADP1 (TPA importer) ( 21 ). Several studies have introduced TPA catabolism into microbes for metabolic engineering applications as well ( 22 – 26 ).…”

mentioning

confidence: 99%

Biochemical and structural characterization of an aromatic ring–hydroxylating dioxygenase for terephthalic acid catabolism

Kincannon

Zahn

Clare

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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Significance More than 400 million tons of plastic waste is produced each year, the overwhelming majority of which ends up in landfills. Bioconversion strategies aimed at plastics have emerged as important components of enabling a circular economy for synthetic plastics, especially those that exhibit chemically similar linkages to those found in nature, such as polyesters. The enzyme system described in this work is essential for mineralization of the xenobiotic components of poly(ethylene terephthalate) (PET) in the biosphere. Our description of its structure and substrate preferences lays the groundwork for in vivo or ex vivo engineering of this system for PET upcycling.

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confidence: 99%

Biochemical and structural characterization of an aromatic ring–hydroxylating dioxygenase for terephthalic acid catabolism

Kincannon

Zahn

Clare

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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“…It has been demonstrated that the PET monomer TPA is suitable for the biosynthesis of several high value chemicals, such as gallic acid, pyrogallol, catechol, muconic acid, vanillic acid, catechol, adipic acid, PHA, and β-ketoadipic acid [106,[139][140][141][142][143]. Since PCA is an important precursor in producing a series of high value aromatic chemicals, the key to the bioconversion of TPA is the acquisition of PCA.…”

Section: Bioconversion Of Tpa To High Value Chemicalsmentioning

confidence: 99%

“…As for producing β-ketoadipic acid from TPA, four sequential metabolic engineering efforts in P. putida KT2440 were performed to directly convert BHET into β-ketoadipic acid [139]. The engineered P. putida is able to not only degrade BHET into TPA and EG, but also convert TPA into 15.1 g/L of β-ketoadipic acid at 76% molar yield in bioreactors [139]. β-ketoadipic can be further polymerized into a nylon-6,6 analog, or other products [147].…”

Section: Bioconversion Of Tpa To High Value Chemicalsmentioning

confidence: 99%

Current Advances in the Biodegradation and Bioconversion of Polyethylene Terephthalate

Yan

Cao

et al. 2021

Microorganisms

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Polyethylene terephthalate (PET) is a widely used plastic that is polymerized by terephthalic acid (TPA) and ethylene glycol (EG). In recent years, PET biodegradation and bioconversion have become important in solving environmental plastic pollution. More and more PET hydrolases have been discovered and modified, which mainly act on and degrade the ester bond of PET. The monomers, TPA and EG, can be further utilized by microorganisms, entering the tricarboxylic acid cycle (TCA cycle) or being converted into high value chemicals, and finally realizing the biodegradation and bioconversion of PET. Based on synthetic biology and metabolic engineering strategies, this review summarizes the current advances in the modified PET hydrolases, engineered microbial chassis in degrading PET, bioconversion pathways of PET monomers, and artificial microbial consortia in PET biodegradation and bioconversion. Artificial microbial consortium provides novel ideas for the biodegradation and bioconversion of PET or other complex polymers. It is helpful to realize the one-step bioconversion of PET into high value chemicals.

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“…Finally, BHET is also a promising precursor for metabolically engineered biocatalysis in microbial hosts, enabling the bioconversion of a plastic waste-derived molecule into valuable product(s). 8,[11][12][13][14][15] A potent BHET esterase (BHE-Tase) would consequently be of signicant importance for PET and/or BHET deconstruction, chemical recycling, and upcycling.…”

Section: Introductionmentioning

confidence: 99%

A flexible kinetic assay efficiently sorts prospective biocatalysts for PET plastic subunit hydrolysis

et al. 2022

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Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440

Cited by 107 publications

References 64 publications

Biochemical and structural characterization of an aromatic ring–hydroxylating dioxygenase for terephthalic acid catabolism

Biochemical and structural characterization of an aromatic ring–hydroxylating dioxygenase for terephthalic acid catabolism

Current Advances in the Biodegradation and Bioconversion of Polyethylene Terephthalate

A flexible kinetic assay efficiently sorts prospective biocatalysts for PET plastic subunit hydrolysis

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