Tandem mass spectrometry: a convenient approach in the dosage of steviol glycosides in Stevia sweetened commercial food beverages

Donna, Leonardo Di; Mazzotti, Fabio; Santoro, Ilaria; Sindona, Giovanni

doi:10.1002/jms.3925

Cited by 10 publications

(5 citation statements)

References 24 publications

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“…En una revisión sistemática de propiedades funcionales y sensoriales, se calificó a la estevia, con efectos superiores a otros edulcorantes y relevantes para el creciente mercado de alimentos naturales en el futuro (14); sus esteviósidos fueron reconocidos como generalmente seguros (GRAS) por la Federación de Drogas y Alimentos (FDA) (30) y aprobados por la Autoridad Europea de Seguridad Alimentaria (EFSA) (31,32). Estos resultados introducen un enfoque analí-tico de identificación de edulcorantes derivados de la hoja de la estevia en refrescos comerciales (33). En este sentido, es importante no perder de vista el impacto de la incorporación de bebidas y alimentos que contienen edulcorantes no nutritivos (NNS) en la calidad de la dieta evaluando beneficios y riesgos (34) para la salud (35).…”

Section: Discussionunclassified

Rol De La Stevia Y L-Carnitina Sobre El Impacto Glicémico De Un Suplemento Nutricional en Adultos

et al. 2017

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Rol de la estevia y L-carnitina sobre el impacto glicémico de un suplemento nutricional en adultos Role of the stevia and L-carnitine of a nutritional supplement on glycemic impact in adults ResumenActualmente la industria alimentaria ha generado interés en edulcorantes no nutritivos, por ejemplo la estevia y en componentes especiales como la L-carnitina, utilizados en formulaciones de suplementos nutricionales para el control glicémico específicos para diabéticos. El presente estudio evaluó el efecto de la estevia y la L-carnitina sobre el índice glicémico (IG) y la carga glicémica (CG) de un suplemento nutricional en 19 sujetos sanos (9 hombres y 10 mujeres), quienes completaron aleatoriamente 3 pruebas de consumo, 1 para el suplemento y 1 para cada producto de referencia: solución glucosada (SG) y pan blanco (PB), obteniendo muestras de sangre a los tiempos 0, 15, 30, 45, 60, 90 y 120 min; para medición de glicemias, e insulina basal y postprandial. El área de incremento bajo la curva de glucosa (IAUC) fue menor para el suplemento 11.778,73 que para los productos de referencia (SG) 13.724,06; (PB) 13.153,56 α = p 0,005. El IG = (62) y la CG = (16) resultaron intermedios y más bajos que el del pan blanco IG = (69) y la CG = (18), sin diferencias en la insulina postprandial. Esto demuestra que este suplemento nutricional formulado con estevia y L-carnitina es capaz de prolongar la respuesta glicémica sin aumentar los requerimientos insulínicos en sujetos sanos. Se requieren estudios específicos en diabéticos para validar si el impacto glicémico es menor que el producto patrón. La presencia de otros nutrientes en la fórmula, influyentes en estos indicadores, no permite inferir que los resultados se deban únicamente al tipo de endulzante utilizado y a la L-carnitina. Key words:Supplement. Stevia. L-carnitine. Glycemia. AbstractCurrently the food industry has generated interest in non-nutritive sweeteners, for example Stevia and in special components such as L-carnitine, used in formulations of nutritional supplements for glycemic control specific for diabetics. The present study evaluated the effect of stevia and L-carnitine on the glycemic index (GI) and glycemic load (CG) of a nutritional supplement in 19 healthy subjects (9 men and 10 women), who randomly completed 3 consumption tests, 1 for the supplement and 1 for each reference product: Glucose solution (SG) and white bread (PB), obtaining blood samples at the 0, 15, 30, 45, 60, 90 and 120 min times; for measurement of blood glucose, basal and postprandial insulin. The increase area under the glucose curve (IAUC) was lower for supplement 11,778.73 than for reference products (SG) 13,724.06; (PB) 13,153.56 α = p 0.005. IG = (62) and CG = (16) were intermediate and lower than white bread IG = (69) and CG = (18), with no difference in postprandial insulin. This demonstrates that this nutritional supplement formulated with stevia and L-carnitine is able to prolong the glycemic response without increasing the insulin requirements in healthy subjects. Specific s...

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Section: Discussionunclassified

Rol De La Stevia Y L-Carnitina Sobre El Impacto Glicémico De Un Suplemento Nutricional en Adultos

et al. 2017

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“…Our research group has done extensive work on the identification and molecular characterization of food compounds [41,42,43]. We have developed environmentally friendly methods for the extraction and further chemical manipulation of natural bioactive molecules [44,45,46,47,48,49,50,51,52], reducing or eliminating the use and generation of harmful substances and solvents aiming to encourage green chemistry [53].…”

Section: Introductionmentioning

confidence: 99%

Sustainable and Selective Extraction of Lipids and Bioactive Compounds from Microalgae

et al. 2019

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The procedures for the extraction and separation of lipids and nutraceutics from microalgae using classic solvents have been frequently used over the years. However, these production methods usually require expensive and toxic solvents. Based on our studies involving the use of eco-sustainable methodologies and alternative solvents, we selected ethanol (EtOH) and cyclopentyl methyl ether (CPME) for extracting bio-oil and lipids from algae. Different percentages of EtOH in CPME favor the production of an oil rich in saturated fatty acids (SFA), useful to biofuel production or rich in bioactive compounds. The proposed method for obtaining an extract rich in saturated or unsaturated fatty acids from dry algal biomass is disclosed as eco-friendly and allows a good extraction yield. The method is compared both in extracted oil percentage yield and in extracted fatty acids selectivity to extraction by supercritical carbon dioxide (SC-CO2).

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“…Sweet-tasting steviol glycosides in plant material and food samples have been determined through different methods, including enzymatic hydrolysis [10], HPTLC [9], capillary electrophoresis [11], near-infrared spectroscopy [12], HPLC with UV detection [13,14], UHPLCevaporative light scattering [15], LC-fluorimeter [16], desorption electrospray ionisation MS [17], UHPLC-MS [18], LC-MS/MS [19][20][21] and LC in combination with hybrid quadrupole time-offlight MS [22,23]. Due to current legislation concerning the composition of the Stevia extract, MS is the analytical technique of choice for the identification and assay of targeted and untargeted species in complex mixtures, such as Stevia extract and Stevia-sweetened food.…”

Section: Introductionmentioning

confidence: 99%

Determination of steviol glycosides in commercial extracts of Stevia rebaudiana and sweeteners by ultra-high performance liquid chromatography Orbitrap mass spectrometry

Gardana

Simonetti

2018

Journal of Chromatography A

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Stevia rebaudiana extracts are used as sweeteners in several countries worldwide. Several extracts of diverse composition are available on the market, and their taste depends on the contents of the various steviol glycosides. This study presents an accurate method for the qualitative and quantitative determination of steviol glycosides in 40 Stevia extracts, 7 sweeteners and 3 Steviasweetened beverages by a UHPLC coupled to an Orbitrap mass spectrometer. The sub-2 µm amide column provided the separation of all the target analytes in a run time of 30 min with high resolution. The effect of different eluent compositions on the ionisation efficiency of the steviol glycosides was studied. The optimal ionisation conditions were achieved in negative mode using 0.05% formic acid. Under this condition, adducts were not found, [M-H]were the main ions and the spontaneous loss of a glucose residue at C19 was reduced. The %RSD for intra-and inter-day precision for all eleven analytes varied from 2.1-4.2% and 3.0-5.1%, respectively. The recoveries from spiked Stevia extract samples were greater than 95% for all analytes. Rebaudioside A was the most abundant, ranging from 23-102%. Nine Stevia extracts and one drink were not compliant with the European Regulation. Isosteviol was under the LOD in all samples and steviol was found in four samples in quantities in the range 0.01-0.03%.

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Tandem mass spectrometry: a convenient approach in the dosage of steviol glycosides in Stevia sweetened commercial food beverages

Cited by 10 publications

References 24 publications

Rol De La Stevia Y L-Carnitina Sobre El Impacto Glicémico De Un Suplemento Nutricional en Adultos

Rol De La Stevia Y L-Carnitina Sobre El Impacto Glicémico De Un Suplemento Nutricional en Adultos

Sustainable and Selective Extraction of Lipids and Bioactive Compounds from Microalgae

Determination of steviol glycosides in commercial extracts of Stevia rebaudiana and sweeteners by ultra-high performance liquid chromatography Orbitrap mass spectrometry

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