Tandem Phosphorothioate Modifications for DNA Adsorption Strength and Polarity Control on Gold Nanoparticles

Zhou, Wenhu; Wang, Feng; Ding, Jinsong; Liu, Juewen

doi:10.1021/am504791b

Cited by 61 publications

(72 citation statements)

References 47 publications

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“…First demonstrated by Kumar et al on colloidal gold, this affinity has been utilized to conjugate phosphorothioate-modified DNA to gold nanoparticles (GNPs) [53,54] and later to QDs [55][56][57] either post or during synthesis. This method is advantageous to link DNA on NPs, but suffers from the limitation that the 'attached' DNA is conformationally distorted and often loses its ability to hybridize complementary DNA [56]. However, this is circumvented by introducing a DNA overhang with phosphodiester backbone, displaying the target sequence [58].…”

Section: Phosphorothioate-modified Dnamentioning

confidence: 99%

Quantum dots–DNA bioconjugates: synthesis to applications

et al. 2016

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Semiconductor nanoparticles particularly quantum dots (QDs) are interesting alternatives to organic fluorophores for a range of applications such as biosensing, imaging and therapeutics. Addition of a programmable scaffold such as DNA to QDs further expands the scope and applicability of these hybrid nanomaterials in biology. In this review, the most important stages of preparation of QD-DNA conjugates for specific applications in biology are discussed. Special emphasis is laid on (i) the most successful strategies to disperse QDs in aqueous media, (ii) the range of different conjugation with detailed discussion about specific merits and demerits in each case, and (iii) typical applications of these conjugates in the context of biology.

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Section: Phosphorothioate-modified Dnamentioning

confidence: 99%

Quantum dots–DNA bioconjugates: synthesis to applications

et al. 2016

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“…We reason that interesting DNA binders might also be produced by using modified DNA, especially those modifications can potentially improve the binding of specific surfaces. For example, phosphorothioate modification might be useful for surfaces with thiophilic metals …”

Section: Discussionmentioning

confidence: 99%

Selection and Screening of DNA Aptamers for Inorganic Nanomaterials

Zhou

Huang

Yang

et al. 2017

Chemistry A European J

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Searching for DNA sequences that can strongly and selectively bind to inorganic surfaces is a long-standing topic in bionanotechnology, analytical chemistry and biointerface research. This can be achieved either by aptamer selection starting with a very large library of ≈10 random DNA sequences, or by careful screening of a much smaller library (usually from a few to a few hundred) with rationally designed sequences. Unlike typical molecular targets, inorganic surfaces often have quite strong DNA adsorption affinities due to polyvalent binding and even chemical interactions. This leads to a very high background binding making aptamer selection difficult. Screening, on the other hand, can be designed to compare relative binding affinities of different DNA sequences and could be more appropriate for inorganic surfaces. The resulting sequences have been used for DNA-directed assembly, sorting of carbon nanotubes, and DNA-controlled growth of inorganic nanomaterials. It was recently discovered that poly-cytosine (C) DNA can strongly bind to a diverse range of nanomaterials including nanocarbons (graphene oxide and carbon nanotubes), various metal oxides and transition-metal dichalcogenides. In this Concept article, we articulate the need for screening and potential artifacts associated with traditional aptamer selection methods for inorganic surfaces. Representative examples of application are discussed, and a few future research opportunities are proposed towards the end of this article.

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“…For example, the thiol‐containing capping ligands including alkyl‐thiolated DNA are widely used to functionalize gold nanomaterials to impart special functionalities given the soft nature of the thiol ligands that have a higher affinity for the soft gold metal. Similarly, incorporation of a phosphorothioate (PS) modification to the phosphodiester backbone can increase its affinity for AuNPs . In the case of AuNR overgrowth in the presence of homo‐oligomeric DNA, the use of A20 induces a red‐shift to the L‐LSPR of the final nanostructures as mentioned above.…”

Section: Insights Into Dna‐encoded Metal Nanoparticle Morphologiesmentioning

confidence: 99%

Discovery of and Insights into DNA “Codes” for Tunable Morphologies of Metal Nanoparticles

Satyavolu

Loh

Tan

et al. 2019

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The discovery and elucidation of genetic codes has profoundly changed not only biology but also many fields of science and engineering. The fundamental building blocks of life comprises of four simple deoxyribonucleotides and yet their combinations serve as the carrier of genetic information that encodes for proteins that can carry out many biological functions due to their unique functionalities. Inspired by nature, the functionalities of DNA molecules have been used as a capping ligand for controlling morphology of nanomaterials, and such a control is sequence dependent, which translates into distinct physical and chemical properties of resulting nanoparticles. Herein, an overview on the use of DNA as engineered codes for controlling the morphology of metal nanoparticles, such as gold, silver, and Pd‐Au bimetallic nanoparticles is provided. Fundamental insights into rules governing DNA controlled growth mechanisms are also summarized, based on understanding of the affinity of the DNA nucleobases to various metals, the effect of combination of nucleobases, functional modification of DNA, the secondary structures of DNA, and the properties of the seed employed. The resulting physical and chemical properties of these DNA encoded nanomaterials are also reviewed, while perspectives into the future directions of DNA‐mediated nanoparticle synthesis are provided.

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Tandem Phosphorothioate Modifications for DNA Adsorption Strength and Polarity Control on Gold Nanoparticles

Cited by 61 publications

References 47 publications

Quantum dots–DNA bioconjugates: synthesis to applications

Quantum dots–DNA bioconjugates: synthesis to applications

Selection and Screening of DNA Aptamers for Inorganic Nanomaterials

Discovery of and Insights into DNA “Codes” for Tunable Morphologies of Metal Nanoparticles

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