Tandem Time-of-Flight Mass Spectrometry with a Curved Field Reflectron

Cotter, Robert J.; Gardner, Ben; Iltchenko, Sergei; English, Robert D.

doi:10.1021/ac0349431

Cited by 43 publications

(31 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[7][8][9] When L-reflectron is tuned for a prompt ion, the time-focusing condition is not met for its dissociation products formed in field-free regions outside the source. Product ion time resolution can be improved either by stepping the reflectron voltage or by using a voltage lift cell.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Reflectron for Kinetic and Mechanistic Studies with Multiplexed Multiple Tandem (MSⁿ) Time-of-flight Mass Spectrometry

Bae¹,

Yoon²,

Moon³

et al. 2010

Bulletin of the Korean Chemical Society

View full text Add to dashboard Cite

Photoexcitation of a precursor ion inside a cell floated at high voltage installed in a tandem time-of-flight (TOF) mass spectrometer provides triple tandem mass spectrometric information and allows kinetic and mechanistic studies. In this work, the factors affecting, or downgrading, the performance of the technique were identified. Ion-optical and computational analyses showed that an optimum instrument could be designed by utilizing a reflectron with linear-plus-quadratic potential inside. Theoretical predictions were confirmed by tests with instruments built with different ion-optical layout. With optimized instruments, masses of intermediate ions in the consecutive dissociation of a precursor ion could be determined with the maximum error of ±5 Da. We also observed excellent agreement in dynamical parameters (critical energy and entropy) for the dissociation of a model peptide ion determined by instruments with different ion-optical layout operated under optimum conditions. This suggests that these parameters can be determined reliably by the kinetic method developed previously when properly designed and operated tandem TOF instruments are used.

show abstract

Section: Introductionmentioning

confidence: 99%

Optimization of Reflectron for Kinetic and Mechanistic Studies with Multiplexed Multiple Tandem (MSⁿ) Time-of-flight Mass Spectrometry

Bae¹,

Yoon²,

Moon³

et al. 2010

Bulletin of the Korean Chemical Society

View full text Add to dashboard Cite

show abstract

“…Tandem mass spectra were obtained on a Kratos (Manchester, England) AXIMA CFR time-of-flight mass spectrometer modified, as described previously [26,27], with a collision cell mounted at the top of the ion source and ion focusing optics in the region ahead of the mass selection gate (Fig. 1).…”

Section: Methodsmentioning

confidence: 99%

Collision energetics in a tandem time-of-flight (TOF/TOF) mass spectrometer with a curved-field reflectron

Ilchenko

Cotter

2007

International Journal of Mass Spectrometry

Self Cite

View full text Add to dashboard Cite

Collisions of fullerene ions (C(60) (+)) with helium and neon were carried out over a range of laboratory energies (3-20 keV) on a unique tandem time-of-flight (TOF/TOF) mass spectrometer equipped with a curved-field reflectron (CFR). The CFR enables focusing of product ions over a wide kinetic energy range. Thus, ions extracted from a laser desorption/ionization (LDI) source are not decelerated prior to collision, and collision energies in the laboratory frame are determined by the source extraction voltages. Comparison of product ion mass spectra obtained following collisions with inert gases show a time (and apparent mass) shift for product ions relative to those observed in spectra obtained by metastable dissociation (unimolecular decay), consistent with impulse collision models, in which interactions of helium with fullerene in the high energy range are primarily with a single carbon atom. In addition, within a narrow range of kinetic energies an additional peak corresponding to the capture of helium is observed for fragment ions C(50) (+), C(52) (+), C(54) (+), C(56) (+) and C(58) (+).

show abstract

“…10) As shown in Fig. 4a, precursor ions are accelerated to an energy of 20 keV and maintain that energy (in the laboratory frame) as they are injected into the collision chamber.…”

Section: R J Cotter Et Almentioning

confidence: 99%

Tandem Time-of-Flight (TOF/TOF) Mass Spectrometry and Proteomics

Cotter

Iltchenko

Wang

et al. 2005

J. Mass Spectrom. Soc. Jpn.

Self Cite

View full text Add to dashboard Cite

ῌ 7 ῌ photodissociation. In our case, however, reduction of the kinetic energy E 1 also reduces the available collision energy.Thus, the curved-field reflectron (CFR) was developed to enable us to utilize the full kinetic energy, resulting from the initial ion acceleration, as the collision energy. 4)The CFR, while developed for the tandem instrument, was also very e#ective for obtaining postsource decay (PSD) product ion mass spectra on a single reflectron instrument. 5), 6)PSD was introduced by Spengler et al. 7) for single mass analyzer instruments with a reflectron, but required changing the reflectron voltage to focus separately di#erent regions of the product ion mass spectrum. The CFR, however, enabled recording of the entire product ion mass range at a single, fixed voltage. Fig. 2. An approach to the design of a tandem TOF mass spectrometer described in ref. 8, in which precursor ions are decelerated prior to entering the collision chamber, and the product ions are then extracted and reaccelerated from a pulsed "second source" before entering the reflectron. Fig. 3. An approach to the design of a tandem mass spectrometer described in ref. 9, in which the product ions with velocities equal to that of their precursor enter a "lift cell" prior to mass analysis.R. J. Cotter, et al.ῌ 8 ῌ Results and Discussion2.1 Tandem mass spectrometers using pulsed extraction and a single reflectron One of the disadvantages of the two reflectron instrument was that considerable post-source decay occurred before the ions entered the first reflectron. The resulting product ions do not arrive at the collision chamber and mass-selector at the same time as their precursor, and so are gated out from the spectrum. Thus, a considerably more sensible approach would be to use a simple, linear TOF as the first mass analyzer, using the fact that precursors and their products will maintain the same velocity and reach the mass-selector at the same time. The combination of a pulsed, linear TOF as a first mass analyzer and a reflectron second mass analyzer is the approach generally used in current tandem TOF instruments, though in some cases deceleration of the ions prior to reaching the collision chamber loses the advantage of recording all mass-selected Fig. 4. An approach to the design of a tandem mass spectrometer described in ref. 10 that does not require deceleration or reacceleration, but focuses product ions in a curved-field reflectron.Fig. 5. Collision-induced dissociation, tandem mass spectra of buckminsterfullerene (C60) at di#erent pressures of helium collision gas.Tandem Time-of-Flight (TOF/TOF) Mass Spectrometry and Proteomics ῌ 9 ῌ post source product ions. For example, in the instrument shown in Fig. 2a and described by Medzihradszky et al. 8) ions formed in the source are accelerated to energies of 20 keV, massselected by a timed ion gate, and then decelerated to 1῍ 2 keV before entering the collision chamber. After the ions are passed through the collision chamber, the remaining precursor and the product ions are rea...

show abstract

Tandem Time-of-Flight Mass Spectrometry with a Curved Field Reflectron

Cited by 43 publications

References 28 publications

Optimization of Reflectron for Kinetic and Mechanistic Studies with Multiplexed Multiple Tandem (MSⁿ) Time-of-flight Mass Spectrometry

Optimization of Reflectron for Kinetic and Mechanistic Studies with Multiplexed Multiple Tandem (MSⁿ) Time-of-flight Mass Spectrometry

Collision energetics in a tandem time-of-flight (TOF/TOF) mass spectrometer with a curved-field reflectron

Tandem Time-of-Flight (TOF/TOF) Mass Spectrometry and Proteomics

Contact Info

Product

Resources

About

Tandem Time-of-Flight Mass Spectrometry with a Curved Field Reflectron

Cited by 43 publications

References 28 publications

Optimization of Reflectron for Kinetic and Mechanistic Studies with Multiplexed Multiple Tandem (MSn) Time-of-flight Mass Spectrometry

Optimization of Reflectron for Kinetic and Mechanistic Studies with Multiplexed Multiple Tandem (MSn) Time-of-flight Mass Spectrometry

Collision energetics in a tandem time-of-flight (TOF/TOF) mass spectrometer with a curved-field reflectron

Tandem Time-of-Flight (TOF/TOF) Mass Spectrometry and Proteomics

Contact Info

Product

Resources

About

Optimization of Reflectron for Kinetic and Mechanistic Studies with Multiplexed Multiple Tandem (MSⁿ) Time-of-flight Mass Spectrometry

Optimization of Reflectron for Kinetic and Mechanistic Studies with Multiplexed Multiple Tandem (MSⁿ) Time-of-flight Mass Spectrometry