Tandem time-of-flight (TOF/TOF) mass spectrometry and the curved-field reflectron

Cotter, Robert J.; Griffith, Wendell P.; Jelinek, Christine

doi:10.1016/j.jchromb.2007.01.009

Cited by 34 publications

(26 citation statements)

References 20 publications

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“…However, TOF-TOF instruments [85] are only combined with MALDI and other laser desorption sources which are not suitable for LC coupling. Recently, a shotgun metabolomic approach has been developed using MALDI-TOF/TOF MS from mouse heart tissues [86].…”

Section: High-energy Collisionsmentioning

confidence: 99%

Mass spectrometry for the identification of the discriminating signals from metabolomics: Current status and future trends

Werner

Heilier

Ducruix

et al. 2008

Journal of Chromatography B

189

141

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Section: High-energy Collisionsmentioning

confidence: 99%

Mass spectrometry for the identification of the discriminating signals from metabolomics: Current status and future trends

Werner

Heilier

Ducruix

et al. 2008

Journal of Chromatography B

189

141

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“…By now, different methods have been established to minimise the kinetic energy distribution during acceleration and its negative effect on resolution. Both MALDI-TOF and ESI-TOF instruments may be equipped with an ion mirror or 'reflectron' to focus ions (Cotter, Griffith, & Jelinek, 2007). Ions drift into the reflectron where they are deflected by an electric field and repelled towards the detector.…”

Section: Mass Analysersmentioning

confidence: 99%

Bacterial Electron Transfer Chains Primed by Proteomics

Wessels¹,

Almeida²,

Kartal³

et al. 2016

Advances in Bacterial Electron Transport Systems and Their Regulation

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Electron transport phosphorylation is the central mechanism for most prokaryotic species to harvest energy released in the respiration of their substrates as ATP. Microorganisms have evolved incredible variations on this principle, most of these we perhaps do not know, considering that only a fraction of the microbial richness is known. Besides these variations, microbial species may show substantial versatility in using respiratory systems. In connection herewith, regulatory mechanisms control the expression of these respiratory enzyme systems and their assembly at the translational and posttranslational levels, to optimally accommodate changes in the supply of their energy substrates. Here, we present an overview of methods and techniques from the field of proteomics to explore bacterial electron transfer chains and their regulation at levels ranging from the whole organism down to the Ångstrom scales of protein structures. From the survey of the literature on this subject, it is concluded that proteomics, indeed, has substantially contributed to our comprehending of bacterial respiratory mechanisms, often in elegant combinations with genetic and biochemical approaches. However, we also note that advanced proteomics offers a wealth of opportunities, which have not been exploited at all, or at best underexploited in hypothesis-driving and hypothesis-driven research on bacterial bioenergetics. Examples obtained from the related area of mitochondrial oxidative phosphorylation research, where the application of advanced proteomics is more common, may illustrate these opportunities.

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“…This peptide is fragmented in a collision chamber. The m/z values of the fragmented peptide can be determined with the second TOF analyzer (Medzihradszky et al, 2000;Cotter, Griffith, & Jelinek, 2007). MALDI-TOF/TOF mass spectrometer identified 101 proteins from isolated synaptic vesicles (Morciano et al, 2005), and was also used for the proteomic analysis of pancreatic zymogen granules (Chen et al, 2006).…”

Section: E Identification Of Secretory Organelles Proteins With Massmentioning

confidence: 99%

Proteomics of regulated secretory organelles

Brunner¹,

Schvartz²,

Couté³

et al. 2009

Mass Spectrometry Reviews

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Regulated secretory organelles are important subcellular structures of living cells that allow the release in the extracellular space of crucial compounds, such as hormones and neurotransmitters. Therefore, the regulation of biogenesis, trafficking, and exocytosis of regulated secretory organelles has been intensively studied during the last 30 years. However, due to the large number of different regulated secretory organelles, only a few of them have been specifically characterized. New insights into regulated secretory organelles open crucial perspectives for a better comprehension of the mechanisms that govern cell secretion. The combination of subcellular fractionation, protein separation, and mass spectrometry is also possible to study regulated secretory organelles at the proteome level. In this review, we present different strategies used to isolate regulated secretory organelles, separate their protein content, and identify the proteins by mass spectrometry. The biological significance of regulated secretory organelles-proteomic analysis is discussed as well.

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Tandem time-of-flight (TOF/TOF) mass spectrometry and the curved-field reflectron

Cited by 34 publications

References 20 publications

Mass spectrometry for the identification of the discriminating signals from metabolomics: Current status and future trends

Mass spectrometry for the identification of the discriminating signals from metabolomics: Current status and future trends

Bacterial Electron Transfer Chains Primed by Proteomics

Proteomics of regulated secretory organelles

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