Tangential high-density electrode insertions allow to simultaneously measure neuronal activity across an extended region of the visual field in mouse superior colliculus

Sibille, Jérémie; Gehr, Carolin; Teh, Kai Lun; Kremkow, Jens

doi:10.1016/j.jneumeth.2022.109622

Cited by 11 publications

(7 citation statements)

References 49 publications

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“…The copyright holder for this preprint this version posted April 8, 2023. ; https://doi.org/10.1101/2023.04.07.536092 doi: bioRxiv preprint chamber for grounding. It is important to keep the dental cement chamber low enough on the anterior and posterior sides to allow for the shallow angle probe and fiber implantations, see Sibille et al (2022) for a detailed description of the method (Sibille et al, 2022b). Craniotomies were made at the marked positions using a dental drill (Johnson-Promident).…”

Section: Surgical Proceduresmentioning

confidence: 99%

“…Craniotomies were made at the marked positions using a dental drill (Johnson-Promident). To allow for the probe insertion at a horizontal angle between 15° and 20°, a small part of the skull at the posterior part of the craniotomy was slightly thinned using the drill to ensure a smooth insertion (Sibille et al, 2022b).…”

Section: Surgical Proceduresmentioning

confidence: 99%

“…In addition, confirmation of probe placement within the SC was done via receptive field (RF) mapping to check the number of channels with RFs. RFs were estimated from the multi-unit activity (MUA) using spike-triggered average (STA) (Sibille et al, 2022b)) (Fig. 1C).…”

Section: Anesthetized Extracellular Recordings and Optogenetic Stimul...mentioning

confidence: 99%

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Retinal input integration in excitatory and inhibitory neurons in the mouse superior colliculusin vivo

Gehr

Sibille

Kremkow

2023

Preprint

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The superior colliculus (SC) is a midbrain structure that contains one of the highest densities of inhibitory neurons in the brain and, together with the thalamocortical visual system, it plays a key role in visually guided behaviors. The SC receives direct inputs from retinal ganglion cells (RGCs) but whether excitatory and inhibitory SC neurons differentially integrate retinal activityin vivois still largely unknown. We recently established an extracellular recording approach using high-density electrodes to measure the activity of RGCs simultaneously with their postsynaptic SC targetsin vivo, that allows addressing how SC neurons integrate RGC activity. Here, we employ this method to study the functional properties and dynamics that govern retinocollicular signaling in a cell-type specific manner by identifying GABAergic SC neurons using optotagging in anesthetized VGAT-ChR2 mice. We measured 305 monosynaptically connected RGC-SC pairs, out of which approximately one third of retinal afferents connect onto inhibitory SC neurons. We show that both excitatory and inhibitory SC neurons receive comparable strong RGC inputs, with functionally similar RGC-SC pairs showing stronger connections. Our results demonstrate that similar wiring rules apply for RGCs innervation of both excitatory and inhibitory SC neurons, which is unlike the cell-type specific connectivity in the thalamocortical system. Contrasting the similar RGC-SC connection strength, we observed that RGC activity contributed more to the activity of postsynaptic excitatory SC neurons than to the activity of postsynaptic inhibitory SC neurons. This implies that the excitatory SC neurons are more specifically coupled to RGC afferent inputs, while inhibitory SC neurons may integrate additional inputs from other sources. Taken together, our study deepens the understanding of cell-type specific retinocollicular functional connectivity and emphasizes that the two major brain areas for visual processing, the visual cortex and the superior colliculus, differently integrate sensory afferent inputs.

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Section: Surgical Proceduresmentioning

confidence: 99%

Section: Surgical Proceduresmentioning

confidence: 99%

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Retinal input integration in excitatory and inhibitory neurons in the mouse superior colliculusin vivo

Gehr

Sibille

Kremkow

2023

Preprint

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“…The probe was then left for ∼10-20 minutes to settle. To ensure that the probe is located within the area that is being visually stimulated, the receptive fields of the multi-unit-activity were mapped using a customized script (Sibille et al, 2022b) before any recording.…”

Section: Methodsmentioning

confidence: 99%

Differential Short-Term Facilitation Of Synaptic Inputs And Spike Transmission At The Retinocollicular SynapseIn Vivo

Teh,

Dossi,

Rouach

et al. 2024

Preprint

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Short-term plasticity (STP) is important for understanding how neuronal circuits can perform different computations. The STP of a neuron pair can be measured directly using paired whole-cell recordings. Besides, the cross-correlation between the presynaptic and postsynaptic neuronal firing is usually used as a proxy for estimating the synaptic properties. However, the relationships between the synaptic inputs and the spiking properties of the postsynaptic neurons during the STP in vivo still remain unclear. Here, we characterized the STP of both synaptic input, measured by the postsynaptic field potential (PFP), and spike transmission at the retinocollicular pathway of mice. We found that the STP of the retinocollicular pathway is mainly facilitating, where the second presynaptic spike induces a larger PFP and higher postsynaptic firing rate than the first presynaptic spike. The facilitation in the postsynaptic firing rate is generally larger than the PFP facilitation. Interestingly, the last postsynaptic spike timing also has a large facilitating effect on the postsynaptic spiking upon receiving a presynaptic input spike. However, the PFP does not depend on the last postsynaptic spike timing, suggesting that there is an input-independent component of spike transmission in STP. Overall, our results indicate that the STP of the retinocollicular pathway is likely a two-stage process, where the spiking plasticity of the postsynaptic neuron could be independent of its inputs.

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“…To ensure the tangential insertion of the Neuropixels probe through the cortical layers 4, and the quantity of thalamocortical axons in the recording, we advise to estimate the potential number of visually driven TCAs. To do so, the short sparse noise stimulus was displayed, and neuronal activity was recorded ( 32 ). The first main verification is to use an adaptation of the “make_fig.m” script from Kilosort ( 33 ) ( SI Appendix , Extended Data Fig.…”

Section: Methodsmentioning

confidence: 99%

Efficient mapping of the thalamocortical monosynaptic connectivity in vivo by tangential insertions of high-density electrodes in the cortex

Sibille,

Gehr,

Kremkow

2024

Proc. Natl. Acad. Sci. U.S.A.

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The thalamus provides the principal input to the cortex and therefore understanding the mechanisms underlying cortical integration of sensory inputs requires to characterize the thalamocortical connectivity in behaving animals. Here, we propose tangential insertions of high-density electrodes into mouse cortical layer 4 as a method to capture the activity of thalamocortical axons simultaneously with their synaptically connected cortical neurons. This technique can reliably monitor multiple parallel thalamic synaptic inputs to cortical neurons, providing an efficient approach to map thalamocortical connectivity in both awake and anesthetized mice.

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Tangential high-density electrode insertions allow to simultaneously measure neuronal activity across an extended region of the visual field in mouse superior colliculus

Cited by 11 publications

References 49 publications

Retinal input integration in excitatory and inhibitory neurons in the mouse superior colliculusin vivo

Retinal input integration in excitatory and inhibitory neurons in the mouse superior colliculusin vivo

Differential Short-Term Facilitation Of Synaptic Inputs And Spike Transmission At The Retinocollicular SynapseIn Vivo

Efficient mapping of the thalamocortical monosynaptic connectivity in vivo by tangential insertions of high-density electrodes in the cortex

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