Tangeretin inhibits fungal ferroptosis to suppress rice blast

Liang, Maojin; Ye, Huijuan; Shen, Qin; Jiang, Xianya; Cui, Guobing; Gu, Wenxiang; Zhang, Lian‐Hui; Naqvi, Naweed I.; Deng, Yi Zhen

doi:10.1111/jipb.13175

Cited by 29 publications

(24 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Meanwhile, tangeretin is a natural flavonoid identified as an ideal candidate for protecting rice against M. oryzae . Tangeretin interferes with M. oryzae conidial ferroptosis by targeting lipid peroxidation mediated by NADPH oxidase (Liang et al, 2021). However, it is not clear whether P. oligandrum induces ferroptosis in oomycetes during the parasitic process.…”

Section: Discussionmentioning

confidence: 99%

Ferroptosis induced by the biocontrol agent Pythium oligandrum enhances soybean resistance to Phytophthora sojae

Yang

Zhang

Zhu

et al. 2022

Environmental Microbiology

View full text Add to dashboard Cite

Ferroptosis is a newly discovered form of cell death accompanied by iron accumulation and lipid peroxidation. Both biotic and abiotic stresses can induce ferroptosis in plant cells. In the case of plant interactions with pathogenic Phytophthora oomycetes, the roles of ferroptosis are still largely unknown. Here, we performed transcriptome analysis on soybean plants treated with the biocontrol agent Pythium oligandrum, a soilborne and non‐pathogenic oomycete capable of inducing plant resistance against Phytophthora sojae infection. Expression of homologous soybean genes involved in ferroptosis and resistance was reprogrammed upon P. oligandrum treatment. Typical hallmarks for characterizing ferroptosis were detected in soybean hypocotyl cells, including decreased glutathione (GSH) level, accumulation of ferric ions, and lipid peroxidation by reactive oxygen species (ROS). Meanwhile, ferroptosis‐like cell death was triggered by P. oligandrum to suppress P. sojae infection in soybean. Protection provided by P. oligandrum could be attenuated by the ferroptosis inhibitor ferrostatin‐1 (Fer‐1), suggesting the critical role of ferroptosis in soybean resistance against P. sojae. Taken together, these results demonstrate that ferroptosis is a P. oligandrum‐inducible defence mechanism against oomycete infection in soybean.

show abstract

Section: Discussionmentioning

confidence: 99%

Ferroptosis induced by the biocontrol agent Pythium oligandrum enhances soybean resistance to Phytophthora sojae

Yang

Zhang

Zhu

et al. 2022

Environmental Microbiology

View full text Add to dashboard Cite

show abstract

“…Sakuranetin is a major phytoalexin flavonoid identified from rice ( Oryza sativa ) that has been shown to inhibit plant pathogens such as Pyricularia oryzae , Gibberella fujikuroi , and Bipolaris oryzae ( Murata et al, 2020 ). Tangeretin substantially delays the formation of Magnaporthe oryzae appressoria, preventing rice blast ( Liang et al, 2021 ). Similarly, sorghum produces flavones as a phytoalexin defense against anthracnose ( Ibraheem et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Cyclocarya paliurus Reprograms the Flavonoid Biosynthesis Pathway Against Colletotrichum fructicola

et al. 2022

View full text Add to dashboard Cite

Cyclocarya paliurus is an endemic Chinese tree species with considerable medicinal, timber, and horticultural value. The anthracnose disease of C. paliurus is caused by the fungal pathogen Colletotrichum fructicola, which results in great losses in yield and quality. Here, resistance evaluation of six cultivars of C. paliurus exhibited varying degrees of resistance to C. fructicola infection, where Wufeng was the most resistant and Jinggangshan was the most susceptive. Physiological measurements and histochemical staining assays showed that the Wufeng cultivar exhibits intense reactive oxygen species accumulation and defense capabilities. A multiomics approach using RNA sequencing and metabolome analyses showed that resistance in C. paliurus (Wufeng) is related to early induction of reprogramming of the flavonoid biosynthesis pathway. In vitro antifungal assays revealed that the flavonoid extracts from resistant cultivars strongly inhibited C. fructicola hyphal growth than susceptible cultivars. Relative gene expression analysis further demonstrated the pivotal antifungal role of C. paliurus flavonoids in targeting Colletotrichum appressorium formation. Together, these results represent a novel resistance mechanism of C. paliurus against anthracnose through the reprogramming of flavonoids, which will lay a foundation for breeding anthracnose-resistant varieties and the application of flavonoid extraction of C. paliurus as a natural antifungal treatment.

show abstract

“…For field trials, one-month-old seedlings of rice cultivar CO39 were transplanted to the blast nursery in Yangjiang, Guangdong Province in March as previously described (Liang, et al, 2021). The field trials used a randomized plot method with three replicates, each with 600 seedlings in 30 m 2 .…”

Section: Field Trialsmentioning

confidence: 99%

Doxorubicin inhibits phosphatidylserine decarboxylase and confers broad-spectrum antifungal activity

Zhou

Zhao

Yang

et al. 2023

Preprint

View full text Add to dashboard Cite

As phospholipids of cell membranes, phosphatidylethanolamine (PE) and phosphatidylserine (PS) play crucial roles in glycerophospholipid metabolism. Broadly, some phospholipid biosynthesis enzymes serve as potential fungicide targets. Therefore, revealing the functions and mechanism of PE biosynthesis in plant pathogens would provide potential targets for crop disease control. We performed analyses including phenotypic characterizations, lipidomics, enzyme activity, site-directed mutagenesis, and chemical inhibition assays to study the function of PS decarboxylase-encoding gene MoPSD2 in rice blast fungus Magnaporthe oryzae. The Mopsd2 mutant was defective in development, lipid metabolism and plant infection. The PS level increased while PE decreased in Mopsd2, consistent with the enzyme activity. Furthermore, chemical doxorubicin inhibited the enzyme activity of MoPsd2 and showed antifungal activity against ten phytopathogenic fungi including M. oryzae and reduced disease severity of two crop diseases in the field. Three predicted doxorubicin-interacting residues are important for MoPsd2 functions. Our study demonstrates that MoPsd2 is involved in de novo PE biosynthesis and contributes to the development and plant infection of M. oryzae and that doxorubicin shows broad-spectrum antifungal activity as a fungicide candidate. The study also implicates that bacterium Streptomyces peucetius, which biosynthesizes doxorubicin, could be potentially used as an eco-friendly biocontrol agent.

show abstract

Tangeretin inhibits fungal ferroptosis to suppress rice blast

Cited by 29 publications

References 48 publications

Ferroptosis induced by the biocontrol agent Pythium oligandrum enhances soybean resistance to Phytophthora sojae

Ferroptosis induced by the biocontrol agent Pythium oligandrum enhances soybean resistance to Phytophthora sojae

Cyclocarya paliurus Reprograms the Flavonoid Biosynthesis Pathway Against Colletotrichum fructicola

Doxorubicin inhibits phosphatidylserine decarboxylase and confers broad-spectrum antifungal activity

Contact Info

Product

Resources

About