Tangzhiqing Granules Alleviate Podocyte Epithelial‐Mesenchymal Transition in Kidney of Diabetic Rats

Xu, Haiyan; Wang, Xu; Liu, Mingming; He, Xueyuan

doi:10.1155/2017/1479136

Cited by 8 publications

(5 citation statements)

References 25 publications

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“…Moreover, in our study, the diabetic group showed severe desmin immunoreaction that indicate the podocytes injury and this was in accordance with [30] who found a significant increase in desmin protein in the kidneys of diabetic rats and referred these changes to the podocyte injury. The renal tubules demonstrated dilated congested blood vessels, vacuolations, desquamation, degenerated mitochondria, pyknotic nuclei, and wide intercellular spaces.…”

Section: Discussionsupporting

confidence: 91%

The protective effect of anise, insulin, and their combination in the nephropathy of diabetic rat model

Ragab

El-Habiby

El-Mehi

et al. 2020

Egyptian Journal of Histology

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Introduction: Among the diabetic complications, the diabetic nephropathy (DN) is the commonest reason for end-stage kidney failure all over the world, and a major indication for dialysis and transplantation. Despite the number of studies on humans and rodents of experimental DN, successful treatment isn't accessible however So, the explore for drugs to prevent the progression of DN has a high priority in biomedical research Aim: To examine the effect of anise on a diabetic nephropathy rat model by streptozotocin and compare its effect with insulin and with their combination. Materials and Methods: Fifty-six adult male albino rats were divided into six main groups. Group I (control), Group II: received aniseed at a dose of 500 mg/kg, Group III (diabetic): received a single IP of 50 mg/kg STZ, Group IV: diabetic rats received aniseed for eight weeks (as group II), Group V: received SC of 2U/Kg once daily of an insulin mixture for eight weeks and Group VI: received aniseed and insulin as the previous doses for eight weeks. At the end of the study, blood samples were collected and the kidney specimens were processed biochemical, histological (light and transmission electron microscopic) and immunohistochemical studies. Morphometric and statistical studies were also performed. Results: Diabetic rats showed disturbed renal architecture involving the corpuscles and tubules, a significant increase in collagen fibers, glomerulosclerosis index, and desmin, VEGF, keratin 18immunoreaction. Administration of aniseed showed impressive improvement of histological structural changes induced by diabetes, insulin showed a mild improvement and use of these agents in combination appeared to have potential advantages especially in reducing of oxidative stress in improving DN Conclusions: A combination of aniseed and insulin have a relevant therapeutic role in the kidney changes induced by diabetes So, they could potentially provide a possible solution for renal changes related to diabetes

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Section: Discussionsupporting

confidence: 91%

The protective effect of anise, insulin, and their combination in the nephropathy of diabetic rat model

Ragab

El-Habiby

El-Mehi

et al. 2020

Egyptian Journal of Histology

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“…EMT of podocytes could be activated in diabetic conditions 15 , 16 and resulted in glomerular fibrosis in ESRD 2 . Previous studies have demonstrated that TGFβ pathway and NFκB pathway played an important role in EMT of podocyte in diabetes mellitus 16 , 17 . However, it is still controversial about how to deal with EMT of podocyte by inhibiting these signaling pathways 18 .…”

Section: Discussionmentioning

confidence: 95%

Apelin inhibited epithelial−mesenchymal transition of podocytes in diabetic mice through downregulating immunoproteasome subunits β5i

Wang

Chang

et al. 2018

Cell Death Dis

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The epithelial−mesenchymal transition (EMT) of podocytes had been reported to be involved in the glomerular fibrosis in diabetic kidney diseases, which was regulated by TGFβ and NFκB pathways. And apelin, an adipokine which is upregulated in diabetic kidney diseases, was reported to be negatively correlated to TGFβ in polycystic kidney disease and attenuate EMT in renal tubular cells. Therefore, it is hypothesized that apelin might inhibit the EMT of podocytes through downregulating the expression and activation of TGFβ/Smad pathway in diabetic kidney diseases. The results showed that apelin in glomeruli of diabetic mice were increased and exogenous apelin inhibited the EMT of podocytes in diabetic mice, which were accompanied with the decreased expression of proteasome subunits β5i. The results from β5iKO mice confirmed that the inhibiting effects of apelin on EMT of podocytes in diabetic mice were dependent on β5i. The results from culture podocytes showed that apelin decreased the degradation of pIκB and promoted the translocation of IκB into nucleus through decreasing the expression of β5i, which would inhibit the promoting effects of NFκB on expression of TGFβ and followed by decreased activation of Smad pathway and EMT in podocytes. In conclusion, apelin might act as an EMT suppressor for podocytes to decrease the process of glomerular fibrosis in diabetic mice.

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“…It also increased the susceptibility of glomerular injury, leading to glomerular sclerosis. Defects in CD2AP caused fusion of the foot processes through deposition of the mesangial matrix, resulting in proteinuria [ 53 – 56 ].…”

Section: Discussionmentioning

confidence: 99%

Erzhi Formula Extracts Reverse Renal Injury in Diabetic Nephropathy Rats by Protecting the Renal Podocytes

Jiang

Yin

Xiang

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Podocytes injury was a crucial factor resulting in diabetic nephropathy (DN). Erzhi formula extract (EZF) was a clinical effective Chinese medicine on DN, but its mechanism was unclear. In this study, the main compounds of EZF and their pharmacokinetics in rat were detected by HPLC-MS/MS. And then, blood glucose, urine protein, renal index, renal microstructural (HE/PAS staining), inflammatory factors (IL-β, TNF-α, IL-6), and protein/mRNA expression related to the function of podocyte (CD2AP and Podocin) in DN rats were investigated after the oral administration of EZF. The concentrations of specnuezhenide and wedelolactone in rat kidney were 7.19 and 0.057 mg/kg, respectively. The Tmax of specnuezhenide and wedelolactone were 2.0 and 1.50 h, respectively. Their Cmax were, respectively, 30.24 ± 2.68 and 6.39 ± 0.05 μg/L. Their AUC(0-∞) were 123.30 ± 2.68 and 16.56 ± 0.98 μg/L⁎h, respectively. Compared with the model group, the blood glucose and the 24-hour urinary protein were significantly decreased (P < 0.05) after 16 weeks' treatment of EZF. The expressions of Podocin and CD2AP protein/mRNA were increased (P < 0. 05). The deteriorate of glomerular morphology was alleviated under the treatment of EZF. EZF prominently decreased the levels of inflammatory factors (P < 0.05). MDA was significantly decreased (P < 0.05) with the significant increase of SOD activity (P < 0.05) in EZF groups. All the results proved that EZF repaired glomerular mesangial matrix, protected renal tubule, and improved renal function in DN rats by upregulating the expression of Podocin and CD2AP protein/mRNA in podocytes.

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Tangzhiqing Granules Alleviate Podocyte Epithelial‐Mesenchymal Transition in Kidney of Diabetic Rats

Cited by 8 publications

References 25 publications

The protective effect of anise, insulin, and their combination in the nephropathy of diabetic rat model

The protective effect of anise, insulin, and their combination in the nephropathy of diabetic rat model

Apelin inhibited epithelial−mesenchymal transition of podocytes in diabetic mice through downregulating immunoproteasome subunits β5i

Erzhi Formula Extracts Reverse Renal Injury in Diabetic Nephropathy Rats by Protecting the Renal Podocytes

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