Tannase Production by Aspergillus niger

Abstract

Abstract:A method for assay of microbial tannase (Tannin acyl hydrolase) based on the formation of chromogen between gallic acid and rhodanine is reported. Maximum Tannase production occurred in the culture broth containing 1-2 % (w/v) tannic acid and 0.05 -0.1 % (w/v) glucose. The pH, incubation period, temperature and Glucose concentration optima of Tannase production was found at 5.5, 36 h, 35 °C and 0.5% respectively. These properties make the enzyme suitable for pollution control and bioprocess industry. This assay is very simple, reproducible, and very convenient, and with it Tannase activity can be measured in relation to the growth of the organism. Aspergillus niger exhibited higher enzyme activity showing about 65 mole percent conversion respectively after a 36 h incubation period. The assay is complete in a short time, very convenient and reproducible.

Improvement of tannase production under submerged fermentation by Aspergillus niger FBT1 isolated from a mangrove forest

“…Similar to our observations, the optimum pH in a case of tannase produced from Aspergillus japonicas was 7.0 (Bradoo et al, 1997). Different results was re-ported by Lokeswari and Jaya Raju (2007) who found that the optimum pH value for tannase produced by A. niger was 5.5.…”

Combined biodegradation and ozonation for removal of tannins and dyes for the reduction of pollution loads

“…The reason for higher amount of biodegradation for the wattle extract, chrome tannin, dye and mixture was due to the fact that tannase was responsible for degradation of tannin, and, peroxidase was responsible for degradation of dyes. It has been found that these enzymes and favorable factors play a crucial role in reducing the pollution problem arising out of these chemicals (Lokeswari and Jayaraju 2007;Batra and Saxena 2005). The selective recovery of chromium by using an environment friendly process of oxidation resulted in recovery of 96.9% chromium.…”

Optimization of Biological Pretreatment of Green Table Olive Processing Wastewaters Using Aspergillus niger

“…The biodegradation is due to their capacity to excrete various extracellular enzymes [13,14]. The efficiency of the biological treatment can be enhanced by carefully selecting the operational conditions that have been observed to influence the decolorization and enzymes' production [7,15,16].…”