Tannic acid induces transcription of laccase genecglcc1in the white-rot fungusCoriolopsis gallica

Abstract

Laccase, a phenoloxidase enzyme secreted by white-rot fungi, has a significant role in the degradation of lignin and environmental pollutants. Coriolopsis gallica is a ligninolytic basidiomycete that produces high levels of this extracellular enzyme. A laccase gene cglcc1 from this fungus has been cloned and sequenced. The capacity of C. gallica to efficiently degrade polyphenols has been successfully applied in our laboratory to the biotreatment and decolorization of several industrial wastewaters. This study focused on the effect of tannic acid, a natural compound widely distributed in plants, on the production of laccase activity by C. gallica. Our results showed an evident increase of extracellular laccase levels when C. gallica was grown in the presence of tannic acid. Concentrations of 50 and 100 microM of this compound increased laccase activity when compared with control samples grown without tannic acid. In addition, we found an increase in laccase transcript levels in C. gallica grown in culture media supplemented with tannic acid. The role of tannic acid was shown to be an inductor of laccase activity in this fungus, due to the enhancement of expression of the laccase gene at the transcriptional level.

Production of tannase by Aspergillus niger HA37 growing on tannic acid and Olive Mill Waste Waters

“…It is noteworthy to mention that several fungal species including Aspergillus strains are able to produce laccase (Bajpai & Patil 1996Scherer & Fischer 1998;Kiiskinen et al 2004). On the other hand there are evidences that tannic acid is able to induce laccase activity in some basidiomycetes fungi (Carbajo et al 2002), where a role for oxidative ligninolytic enzymes in the degradation of phenolic fraction of OMWW was proposed (Sayadi & Ellouz 1995;Jaouani et al 2003). Confirmation of the presence of laccase in A. niger HA37 still awaits further investigation.…”

Laccase and polyphenol oxidase activities of marine cyanobacteria: a study with Poly R-478 decolourization

“…Previous studies demonstrated the role of laccases and PPOs in degradation of these compounds, and the activities of these enzymes were found to be enhanced by various inducers (Dekker et al 2001;Maceiras et al 2001;Carbajo et al 2002;DSouza et al 2006). In the present study also caffeic acid, guaiacol, tannic acid and veratryl aldehyde resulted in induced isoform at Rm 0.156 compared with uninduced cells; however, its relative intensity was higher when using veratryl aldehyde (Fig.…”

Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design

“…Our findings revealed that laccase activity enhancement also occurred in tomato juice medium without external inducers, as the activity achieved was higher (11 000 IU/L) than that produced in the optimized glucose-meat peptone medium and CuSO 4 . This medium served as a source of carbon and nitrogen for the growth of P. sanguineus and also might induce the enzyme production on account of its phenolic compounds as flavonoids and tannic acid (Carbajo et al, 2002;Ullrich et al, 2005).…”